Abstract
Mannan and xylan present in bleached softwood dissolving pulp were found to be partially resistant to hemicellulases even after repeated enzyme treatment. Despite the additional effect of an endoglucanase from Gloeophyllum sepiarium, which increased the␣accessibility of mannan and xylan to a mannanase from Sclerotium rolfsii and to a xylanase from Thermomyces lanuginosus, the enzyme mixture solubilized only half of the hemicellulose present in the pulp. Half of the remaining hemicellulose present in the pulp appeared to be entrapped within the cellulose matrix while the other half was associated with lignin-carbohydrate complexes. The latter hemicellulose portion was isolated and characterized. Chromatography and spectroscopic techniques revealed the presence of two types of lignin-carbohydrate complex, a galactoglucomannan-lignin complex (degree of polymerization DP 50–60) and a xylan-lignin complex (DP >200).
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Received: 8 December 1997 / Received revision: 30 April 1998 / Accepted: 8 May 1998
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Gübitz, G., Stebbing, D., Johansson, C. et al. Lignin-hemicellulose complexes restrict enzymatic solubilization of mannan and xylan from dissolving pulp. Appl Microbiol Biotechnol 50, 390–395 (1998). https://doi.org/10.1007/s002530051310
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DOI: https://doi.org/10.1007/s002530051310