Abstract
Sterilised and non-sterilised soils contaminated with pentachlorophenol (PCP) were inoculated with solid substrate cultures of Lentinula edodes LE2 (“shiitake” mushroom) to simulate monoculture bioremediation treatments and treatments in which the fungus competes with natural microflora. With monocultures of L. edodes, rates of PCP depletion were rapid for the initial 4 weeks and, although thereafter the rate decreased, 99% biotransformation was obtained in 10 weeks. In mixed culture, PCP biotransformation by L.␣edodes was markedly slower and only 42% of the PCP was depleted after 10 weeks. Maximal rates of PCP transformation, biomass (ergosterol) accumulation and oxidative enzymes (phenol oxidase and manganese-peroxidase) production were observed after 2 weeks of incubation. In monocultures, phenol oxidase activity was 195.5 U g−1 and Mn-peroxidase 138.4 U g−1. In mixed cultures, fungal enzyme activities were markedly lower: 70.33 U g−1 for phenol oxidase and 85.0 g−1 for Mn-peroxidase. Analyses of soil metabolites after 10 weeks revealed that monocultures of L.edodes had eliminated both PCP and pentachloroanisole. Pentachloroanisole, however, was detected in soils with the mixed microflora. Both dechlorination and mineralisation of the xenobiotic compound were effected by L. edodes LE2.
Article PDF
Similar content being viewed by others
Explore related subjects
Discover the latest articles, news and stories from top researchers in related subjects.Avoid common mistakes on your manuscript.
Author information
Authors and Affiliations
Additional information
Received: 7 April 1997 / Accepted: 14 June 1997
Rights and permissions
About this article
Cite this article
Okeke, B., Paterson, A., Smith, J. et al. Comparative biotransformation of pentachlorophenol in soils by solid substrate cultures of Lentinula edodes . Appl Microbiol Biotechnol 48, 563–569 (1997). https://doi.org/10.1007/s002530051097
Issue Date:
DOI: https://doi.org/10.1007/s002530051097