Abstract
β-Galactosidases from Lactobacillus delbruekii subsp. bulgaricus 20056, Lb. casei 20094, Lactococcus lactis subsp. lactis 7962, Streptococcus thermophilus TS2, Pediococcus pentosaceus PE39 and Bifidobacterium bifidum 1901 were partially purified. The rate of hydrolysis of lactose given by the predominant β-galactosidase activity from each of the bacteria studied was in all cases enhanced by Mg2+, while the effect of K+ and Na+ differed from strain to strain. The β-galactosidases from all strains also catalysed transgalactosylation reactions. The types of oligosaccharides produced appeared to be very similar in each case, but the rates of their production differed. All the β-galactosidases were also capable of hydrolysing galactosyl-lactose although, unlike the other bacteria studied, Lb. delbruekii subsp. bulgaricus 20056 and Lc. lactis subsp. lactis 7962 were unable to utilise galactosyl-lactose as a carbon source for growth.
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Received: 4 October 1995/Received revision: 5 March 1996/Accepted 11 March 1996
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Garman, J., Coolbear, T. & Smart, J. The effect of cations on the hydrolysis of lactose and the transferase reactions catalysed by β-galactosidase from six strains of lactic acid bacteria. Appl Microbiol Biotechnol 46, 22–27 (1996). https://doi.org/10.1007/s002530050778
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DOI: https://doi.org/10.1007/s002530050778