Abstract
Laccase, purified from Coriolus versicolor, removed pentachlorophenol (PCP) from solution at pH 5, depending on initial PCP concentration and amount of laccase. With 100 units of laccase, 100% of 25 μg ml−1 PCP and 60% of 200 μg ml−1 PCP were removed respectively over 72 h. No free chloride was released in the reaction. In reaction with 100 μg PCP, products were primarily polymers (about 80,000 MW) with only 2–3 pg of o- and p-chloranils formed. Polymers were stable to acid hydrolysis and no release of PCP, or other low-molecular-weight products, was detected over several weeks. Laccase has a potential use in the biotreatment of aqueous effluents containing PCP, with polymerised products being removed from solution due to their high molecular weight.
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Received: 7 June 1999 / Received revision: 18 August 1999 / Accepted: 2 September 1999
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Ullah, M., Bedford, C. & Evans, C. Reactions of pentachlorophenol with laccase from Coriolus versicolor . Appl Microbiol Biotechnol 53, 230–234 (2000). https://doi.org/10.1007/s002530050013
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DOI: https://doi.org/10.1007/s002530050013