Abstract
While high-affinity H2-oxidizing bacteria (HA-HOB) serve as the main sink of atmospheric H2, the ecology of this specialist functional group is rather unknown due to its recent discovery. The main purpose of our study is to provide the first extensive survey of HA-HOB in farmland, larch, and poplar soils exposed to 0.5 and 10,000 ppmv H2. Using qPCR and qRT-PCR assays along with PCR amplicon high-throughput sequencing of hhyL gene encoding for the large subunit of high-affinity [NiFe]-hydrogenases (HAH), we found that hhyL gene expression ratio explained better variation in measured H2 oxidation rates than HA-HOB species richness. Carbon, nitrogen, pH, and bacterial species richness appeared as the most important drivers of HA-HOB community structure. Our study also highlights the need to cultivate HA-HOB due to the huge gap in current genomic databases.
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State-of-the-art atmospheric chemistry-transport models indicate that nearly 70% of atmospheric H2 losses are caused by microbial H2-oxidizing metabolism in upland soils [1]. Microbes accountable for this crucial ecosystem service remained unknown until Streptomyces spp. harboring high-affinity [NiFe]-hydrogenases (HAH) were discovered [2, 3]. Genome database mining and measurement of H2 uptake in pure cultures demonstrated that other Actinobacteria such as Mycobacterium spp. [4] and Rhodococcus spp. [5], and Acidobacteria [4, 6], have the ability to scavenge trace levels of H2. The ecology of this functional group is fairly unknown, yet soil moisture, carbon content, and pH were identified as key drivers of high-affinity H2 oxidation in soils [7]. While soil moisture limits H2 diffusion [8], the stimulating effect of carbon and pH on H2 uptake is not fully understood. They both act as ecological niche filters selecting or activating high-affinity H2-oxidizing bacteria (HA-HOB), resulting in higher uptake rates in forests than in grassland and farmland ecosystems [9]. Our study aims to provide the first extensive survey of HA-HOB in soil and relate their diversity profile to soil H2 uptake rate and physicochemical properties. We used total genomic DNA and RNA extracts from a previous study in which soil samples encompassing three land use types were exposed to either ambient H2 (aH2; 0.5 ppmv H2) or elevated H2 (eH2; 10,000 ppmv H2) treatments during 15 days in a dynamic microcosm chamber unit [10]. Briefly, soil samples were collected in a 12-year-old larch plantation, a 10-year-old poplar plantation, and a farmland consisting of potato and maize rotation. The dynamic microcosm chamber unit consisted of a gas mixer and a gas distribution network that continually supplied soil microcosms with synthetic air mixtures, containing precise H2 stoichiometric ratios, and vented them to the atmosphere. Distribution of HA-HOB was examined through qPCR/qRT-PCR and PCR amplicon sequencing of hhyL gene, encoding for the large structural subunit of HAH. We hypothesized that bacterial species richness varies in tandem with HA-HOB species richness and that land use defines HA-HOB abundance and diversity.
The abundance of hhyL gene estimated by qPCR ranged from 108 to 109 gene copies g−1 (dw) (Table 1), akin to previous soil surveys [11]. Single linear regression analysis showed that hhyL expression ratios (number of transcripts divided by gene count detected by qRT-PCR and qPCR) explained variations in high-affinity H2 oxidation rates (R 2 = 0.45, p = 0.04, n = 18), which is comparable to the resolution of a previous approach relying on the relative abundance of HA-HOB estimated by hhyL and 16S ribosomal RNA (rRNA) gene qPCR analysis [12]. On the other hand, neither hhyL gene transcripts (R 2 = 0.21, p > 0.05) nor hhyL gene counts (R 2 = 0.00, p = 0.98) alone reflected measured activities. Sequences of PCR-amplified hhyL gene obtained from Illumina MiSeq (2× 250-bp configuration) sequencing platform were clustered into 664 OTUs using a 99% identity cutoff (Supplemental Method 1). This stringent threshold was selected as a trade-off to delineate HA-HOB species, considering both unavoidable technical limitations (i.e., sequencing error rate [13] and potential errors introduced by paired-end assembly [14]) and frequent lateral transfers resulting in considerable underestimations of HA-HOB richness [11]. An extensive database of [NiFe]-hydrogenase large subunit genes was used for phylogenetic analysis of hhyL OTUs (Supplemental Method 1). Consensus phylogenetic tree of reference sequences displays the expected clusters outlining [NiFe]-hydrogenases encompassing group 1 to 5 (Fig. 1a). All OTUs were accurately assigned to group 5 cluster, establishing the specificity of the oligonucleotides targeting hhyL gene. However, only nine OTUs were closely associated with reference sequences (Fig. S1). The most abundant OTU genotype from our dataset (OTU_1, 20–40% abundance) has an uncultivated clone retrieved from deciduous forest soil in Germany as its closest neighbor (clone MS12, 100% similarity). Similarly, OTU_858 was classified in a cluster comprising environmental clones LS15 and LS27 (97% similarity) detected in Finnish peatlands, although seven other OTUs were affiliated to clusters comprising known bacterial species. OTU_294 was related to Rhodococcus sp. (89% similarity) and OTU_237 encompassed a cluster comprising Streptosporangium roseum and the environmental clone MS20 (92% similarity) originating from deciduous forest soil in Germany. OTU_81 and OTU_423 were affiliated to Streptomyces spp. (97–99% similarity), while OTU_869, OTU_1305, and OTU_1312 were related to Conexibacter woesei (76–81% similarity). Considering this, all HA-HOB harboring hhyL genes within this survey remain undiscovered.
The number of observed OTUs was comparable to species richness estimators (Table 1), indicating sufficient sequencing effort to cover HA-HOB diversity. Neither pairwise correlation between 16S rRNA and hhyL genes richness (Pearson, P = 0.4788) nor evenness (Pearson, P = 0.0708) showed significant covariation, suggesting that HA-HOB diversity is independent of bacterial species richness in soil. Furthermore, H2 treatments caused no significant alteration in HA-HOB richness or evenness (Table 1). Similarly, the impact of eH2 exposure on hhyL OTU distribution profiles was undiscernible at the community level (PERMANOVA, p = 0.254). Nevertheless, differential analysis of OTU count data (Supplemental Method 1) indicated that 27 OTUs were affected by eH2 exposure, mostly with higher relative abundance in eH2-treated soils, with contradicting patterns between land uses (Supplemental Table 2). This agrees with previous observations in which microbial response to eH2 exposure was idiosyncratic due to the most influential role of abiotic factors such as carbon, nitrogen, and pH on microbial communities [10, 16]. Genotypes showing higher abundance under eH2 could be representatives of HOB possessing both HAH (i.e., group 5 hydrogenase) and low-affinity [NiFe]-hydrogenases (e.g., group 3 hydrogenase), like Mycobacterium smegmatis [17]. Indeed, multiple hydrogenases provide greater flexibility to switch between survival mixotrophy supported by HAH, at low H2 mixing ratios, and lithoautotophic growth under elevated H2 mixing ratios. Bacteria possessing only HAH are expected to be hindered by eH2 exposure [18]. Although the exact biochemical mechanism is unknown, such potential substrate inhibition on HA-HOB metabolism was supported by lower hhyL gene expression ratio in eH2-exposed soils (Table 1).
Neither HA-HOB species richness nor evenness was distinguishable across the three land use types (Table 1). In contrast to H2 exposure treatments, land use was a significant driver of HA-HOB community structure, explaining 62% of the variance in hhyL gene distribution (PERMANOVA, p < 0.001). Most OTUs were land use-specific (Fig. 1b), yet 106 OTUs common to the three land uses were most abundant, since they represented up to 88% of hhyL genes in any given sample. A parsimonious redundancy analysis (RDA) was then computed to pinpoint which biotic and abiotic parameters measured in soil microcosms [10] acted as environmental filters for hhyL gene distribution profiles. Nearly 50% variance in HA-HOB community structure was explained by soil carbon and nitrogen contents, pH, and bacterial species richness (Fig. 2). Several OTUs also discriminated land uses in the reduced space, including OTU_15 which was more abundant in farmland soils. The important contribution of carbon and pH in explaining HA-HOB community structure complies with previous studies investigating environmental drivers of H2 oxidation activity in soil [7, 12, 19].
In short, this study displayed the diversity of soil HA-HOB and the crucial need to cultivate more HA-HOB in order to fill the gap in genomic databases, which are currently not suited to depict soil microbiomes [20].
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Acknowledgements
This work was supported by a Natural Sciences and Engineering Research Council of Canada Discovery grant to P.C. The authors acknowledge use of McGill University and Génome Québec Innovation Centre for the preparation of hhyL gene libraries and sequencing services. S.P.-C. and M.K. are grateful to the Fondation Universitaire Armand-Frappier INRS for their Ph.D. scholarship.
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Raw sequence reads of hhyL genes from PCR amplicon sequencing were deposited in the Sequence Read Archive of the National Center for Biotechnology Information under BioProject PRJNA382186.
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Piché-Choquette, S., Khdhiri, M. & Constant, P. Survey of High-Affinity H2-Oxidizing Bacteria in Soil Reveals Their Vast Diversity Yet Underrepresentation in Genomic Databases. Microb Ecol 74, 771–775 (2017). https://doi.org/10.1007/s00248-017-1011-1
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DOI: https://doi.org/10.1007/s00248-017-1011-1