Abstract
Wetlands are major sources of carbon dioxide, methane, and other greenhouse gases released during microbial degradation. Despite the fact that decomposition is mainly driven by bacteria and fungi, little is known about the taxonomic diversity of bacterial communities in wetlands, particularly Sphagnum bogs. To explore bacterial community composition, 24 bogs in Vermont and Massachusetts were censused for bacterial diversity at the surface (oxic) and 1 m (anoxic) regions. Bacterial diversity was characterized by a terminal restriction fragment length (T-RFLP) fingerprinting technique and a cloning strategy that targeted the 16S rRNA gene. T-RFLP analysis revealed a high level of diversity, and a canonical correspondence analysis demonstrated marked similarity among bogs, but consistent differences between surface and subsurface assemblages. 16S rDNA sequences derived from one of the sites showed high numbers of clones belonging to the Deltaproteobacteria group. Several other phyla were represented, as well as two Candidate Division-level taxonomic groups. These data suggest that bog microbial communities are complex, possibly stratified, and similar among multiple sites.
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Acknowledgments
This work was supported by funding from NSF (EPS-0082977). The authors would like to thank Aaron M. Ellison for sharing data as well as his extensive knowledge of bog ecology and geography. We are grateful to Don Stratton for assistance with the statistical analysis and Carol Tudhope and Grant Henderson for sample preparation and data organization. Kevin Penn is thanked for performing DNA sequencing and analysis.
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Morales, S.E., Mouser, P.J., Ward, N. et al. Comparison of Bacterial Communities in New England Sphagnum Bogs Using Terminal Restriction Fragment Length Polymorphism (T-RFLP). Microb Ecol 52, 34–44 (2006). https://doi.org/10.1007/s00248-005-0264-2
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DOI: https://doi.org/10.1007/s00248-005-0264-2