Abstract.
Among different treatments assayed, a mix of a nonionic detergent (5% Tween-20) with 0.5 m NaCl was found to solubilize a large part of the calmodulin-dependent NAD+ kinase bound to the inner mitochondrial membrane. It also stimulated its activity by increasing 7 times the maximal velocity. Activity stimulation was also observed with phosphatidylcholine, phosphatidylethanolamine and with reductants (HSO3 and DTT). This solubilized NAD+ kinase and the calmodulin-dependent cytosoluble isoform displayed distinct molecular masses, as well as different kinetic parameters. We propose that solubilization of membrane-bound NAD+ kinase could occur in vivo in Avena sativa and could generate a soluble isoform.
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Received: 6 November 2000/Revised: 18 April 2001
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Pou de Crescenzo, MA., Gallais, S., Léon, A. et al. Tween-20 Activates and Solubilizes the Mitochondrial Membrane-bound, Calmodulin Dependent NAD+ Kinase of Avena sativa L.. J. Membrane Biol. 182, 135–146 (2001). https://doi.org/10.1007/s0023201-0039-8
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DOI: https://doi.org/10.1007/s0023201-0039-8