Abstract
Motor skills are commonly acquired through practice. This process not only involves acquisition of the particular task demands but also requires overcoming pre-existing modes. In the present study, interactions between new and intrinsic dynamics were evaluated. Accordingly, bimanual finger tapping with a 2:1 ratio was performed according to two training schedules: continuous (consecutive trials) and interrupted (non-consecutive trials with intermediate 1:1 in-phase performances). In addition, in-phase and anti-phase were probed before and after training. Behavioral output was assessed by means of temporal accuracy and variability, whereas neural activation patterns were determined by EEG coherence. Results showed that continuous practice resulted in improved performance with reduced coherence across the motor network. For interrupted practice, behavioral execution ameliorated, although it was inferior to performance with continuous practice. In terms of neural changes, the degree of intrahemispheric and midline connectivity did not reduce with interrupted practice, whereas interhemispheric connectivity increased. This signifies that short-term motor consolidation of the 2:1 task was disrupted due to intermediate performance of the in-phase mode. Furthermore, the probed in-phase and anti-phase pattern showed no behavioral changes, although neural alterations occurred that depended on training schedule and coordination mode. Overall, the observations illustrate bidirectional interactions between new and inherent dynamics during motor acquisition, raising issues about effective methods for learning skills and scheduling of practices in neurorehabilitation.
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Introduction
Bimanual routines are part of our daily-life activities and at times involve a high degree of complexity such as playing a musical instrument. Typically, compound acts can be performed following practice during which the behavior gains accuracy and stability. At the same time, learning-related changes occur at the neural level, reflecting greater functional efficiency and advanced movement control (Debaere et al. 2004; Doyon and Benali 2005; Haslinger et al. 2004; Puttemans et al. 2005). Overall, it is acknowledged that motor learning characterizes those internal processes that result in a relatively permanent change of skilled performance (Schmidt and Lee 2005), thus distinguishing it from adaptation or other short-lived effects. Of note is that motor learning not only necessitates the acquisition of the particular task demands but also requires overcoming the pre-existing modes (Zanone and Kelso 1992). Indeed, an inability to overrule intrinsic tendencies associates with inappropriate habits during skill acquisition (Walter et al. 1997), which may become evident through patterns of interference (Semjen 2002).
One well-known example of intrinsic behavior is the tendency towards spatiotemporal coupling during rhythmic bimanual actions (Franz et al. 1991; Kelso et al. 1981; Swinnen et al. 1991). This preference during which both limbs move at similar tempo with synchronization at the reversal points integrates the in-phase (symmetrical) and anti-phase (asymmetrical) mode. Typical is that these configurations can be performed with high accuracy and low variability without practice (Kelso 1984). Based on the previous, it is argued that bimanual tasks are particularly valuable for evaluating the acquisition of new coordination patterns and for assessing influences of pre-existing modes. In this respect, earlier work has shown that bimanual motor training at other phases is accompanied by (temporal) destabilization of the intrinsic modes (Fontaine et al. 1997; Kelso and Zanone 2002; Rémy et al. 2008), whereas acquisition of the new task involves overcoming those intrinsic preferences (Summers 2002). This indicates that pre-existing tendencies influence the new behavior but in return are affected by the to-be-learned task.
In general, the effect of practice has received considerable attention in motor learning studies, and researchers have employed a variety of tasks, contexts and paradigms, practice techniques as well as schedules (e.g., Bays et al. 2005; Kostrubiec et al. 2006; Krakauer et al. 2006; Shea et al. 1979; Vangheluwe et al. 2006; Wulf et al. 1994). This line of research has pointed to two factors that in particular seem to impact on the training conditions: (1) the number of tasks or task variations practiced and (2) the order in which the tasks are trained. The present study builds upon this knowledge by evaluating the training of a well-defined motor task according to two practice schedules that integrate pre-existing behavior. In particular, acquisition of a bimanual finger tapping task with a 2:1 frequency ratio is examined. This assessment is based on behavioral findings that have shown that this assignment involves intermittent periods of attraction towards pre-existing modes (Summers 2002). Furthermore, the 2:1 task permits a strategy that involves both effectors to alternate between simultaneous and separate responses. Although this manner of implementation represents a simplified tactic to comply with the coordinative demands, it introduces an alternating process of facilitation during which both effectors move together (in-phase) and inhibition during which one effector moves while the other is prevented from moving. In this work, two types of evaluations are being made in order to determine competition between new and pre-existing dynamics. First, it is examined how practice of a new task (multifrequency = 2:1 coordination) affects its neural activity and that of the intrinsic modes (isofrequency = in-phase and anti-phase coordination). Second, it is assessed to what extent the training schedule (continuous = consecutive 2:1 practice vs. interrupted = non-consecutive 2:1 practice with intermediate in-phase performances) evokes distinct changes in the task’s neural regulation. As the in-phase mode partly operates as an attractor and partly as a distractor for the multifrequency task requirements, the suggestion is made that intermediate in-phase executions will cause interference with the 2:1 training schedule. To assess the neural dynamics of motor acquisition and its adaptability, the data analysis focuses on EEG coherence, which expresses functional communication between brain areas.
Methods
Participants and tasks
Two groups of eight right-handed individuals (group1: age: 26 ± 4 years, group 2: age: 25 ± 3 years, 2 males and 6 females in each group) as determined by the Edinburgh handedness inventory (Oldfield 1971) participated in the experiment. Inventory scores from both groups were not significantly different from one another (P > 0.05). In accordance with the declaration of Helsinki, the participants gave informed consent to take part in the study, which was approved by the local ethics committee. The participants were randomly assigned to a group and were asked to perform bimanual tapping using their index fingers on a keyboard according to a 2:1 mode (new behavior), in-phase or anti-phase mode (pre-existing behavior). For the 2:1 task, which required tapping with one finger at twice the rate of the other finger, subjects were asked to use a performance strategy that involved moving both fingers simultaneously for one tap, followed by a subsequent tap of the fast finger while holding the slow finger stationary at peak upward position. In terms of timing arrangement, the non-dominant (left) finger adopted the faster tempo as the dominant (right) finger took on the slower tempo. According to this scheme, the timing demands of the non-dominant effector prevail, making the bimanual performance more difficult than the reverse arrangement (Semjen 2002). Although both effectors have distinct timing goals, it is assumed that the timekeeper is established at the frequency of the faster moving one (Semjen 2002). Timing was externally paced, and the metronome beat was set at 545 ms, which guided the fast tempo during the 2:1 trials and the tempo during the in-phase as well as anti-phase trials. There were about 60 taps per trial (metronome guided).
The training schedules which consisted of a pre, practice and post session are depicted in Fig. 1. Both groups received distinct practice of the 2:1 task. Group 1 performed 2:1 trials (n = 10) that were interspersed with in-phase trials (n = 4) in order to interrupt motor practice, whereas group 2 executed 2:1 trials (n = 14) repeatedly in order to optimize motor practice. Both groups completed similar pre and post sessions. In particular, the pre session consisted of in-phase and anti-phase trials (counterbalanced order across subjects) for probing their intrinsic nature. The post session comprised retention trials that followed training of the 2:1 task after a 10 min break. This session included 2:1, in-phase and anti-phase trials. During retention, the 2:1 task was always performed first followed by the in-phase and anti-phase trials (counterbalanced order across subjects). There were small breaks in between trials for avoiding fatigue and loss of attention. Participants were told in advance of the upcoming task requirements. They were advised to tap as smoothly as possible. A rest condition was also recorded that comprised listening to the tones of the metronome.
EEG recordings and data analysis
Continuous EEG was recorded using the Electrical Geodesics Inc. 128-channel system, and data processing was carried out using BESA software (MEGIS Software GmbH, Gräfelfing, Germany). EEG signals were amplified, band-pass filtered 0.05–100 Hz, and sampled at 250 Hz with a vertex reference. Epochs contaminated by artifacts such as eye movements and EMG-related activity were corrected for or rejected after baseline correction.
EEG coherence was used to assess functional connectivity between brain areas in the frequency domain, and was estimated by means of complex demodulation set to a frequency resolution of 2 Hz and temporal resolution of 25 ms. Background coherence acquired during rest was subtracted from coherence obtained during motor conditions. This method, which gives an estimate of task-related coherence, reduces the effects of volume conduction, between-subject differences as well as between-electrode variability, and minimizes the bias introduced by the reference electrode. As a normalized measurement of coupling between two signals at any given frequency, coherence varies between 0 (no correlation) and 1 (perfect correlation).
To measure indices of cortical activity, a region of interest approach was adopted that focused on a restricted number of electrodes. The electrodes were selected based on earlier EEG studies of movement control (Hummel et al. 2002; Serrien 2009) and were estimated to overlie premotor, sensorimotor, superior parietal and mesial areas, including supplementary motor area (SMA). The division of electrodes resulted in the following connectivity groupings: intrahemispheric left (FC3-C3, FC3-CP3, FC3-P3, C3-CP3, C3-P3, CP3-P3), intrahemispheric right (FC4-C4, FC4-CP4, FC4-P4, C4-CP4, C4-P4, CP4-P4), interhemispheric (FC3-FC4, C3-C4, CP3-CP4, P3-P4) and midline (FCz-CPz). Coherence was evaluated in the beta frequency band (>12–30 Hz) due to its importance for motor behavior (Gerloff et al. 1998; Serrien et al. 2003). Before statistical operations were conducted, coherences were transformed using the inverse hyperbolic tangent to stabilize variances. Separate analyses were conducted for the different connectivity groupings. In addition, EEG task-related power (obtained by subtracting rest from the corresponding motor conditions) was measured in the beta band at the individual electrodes, and stabilized by logarithmic transformation. Subsequently, power was analyzed in conjunction with coherence measurements in order to evaluate whether changes in power could have contributed to the modulations in coherence. Non-significant effects would indicate that the motor system effectively responded by adjusting information flow between cortical regions. Mean ± SD scores are presented in “Results”.
As illustrated in Fig. 1, both groups performed three consecutive sessions, pre, practice and post. The main analyses were conducted for both groups separately and included the pre session (in-phase, anti-phase), practice session (start trial 1, end trial 14) and post session (2:1, in-phase, anti-phase). Furthermore, analyses were carried out that contrasted both groups on practice trial 10 of the 2:1 task, which involved trial 14 for group 1 (interrupted practice) and trial 10 for group 2 (continuous practice). Also, the start practice trial of both groups was compared.
Behavioral recordings and analysis
E-Prime software (Psychology Software Tools Inc., Pittsburgh, USA) was used to record the cycle durations in the various conditions. The metronome pace of 545 ms provided a reference signal for all motor tasks. The analysis included temporal accuracy with respect to the 2:1 and 1:1 ratio between both hands. Moreover, the deviation from the ideal value (1 or 2) was estimated for the corresponding finger taps, and accordingly averaged per trial in order to capture goal achievement of the coordinative demands. As a measurement of temporal variability, the coefficient of variation (CV) of both hands was computed as the standard deviation in timing divided by the mean tempo.
Statistical analysis
The data were analyzed using the Statistica software (StatSoft Inc., Tulsa, USA). Adjustments were made in case of violation of the sphericity assumption by using the Greenhouse–Geisser procedure. Post hoc testing included corrections with respect to multiple comparisons.
Results
Group 1: Interrupted practice of the 2:1 task
This group carried out the 2:1 task (n = 10 trials) while in-phase movements (n = 4 trials) were intermediately performed to disturb motor practice (Fig. 1).
Practice of the 2:1 task
Behavioral timing
Accuracy and variability scores were calculated (Fig. 2a). Accuracy: A one-way ANOVA on session (start practice, end practice, post) revealed a significant effect, F(2,14) = 4.34, P < 0.05. Post hoc analysis showed that timing accuracy at the start of practice was lower than that obtained at the end of practice and at the post session (P < 0.05 for both). Variability: A two-way ANOVA on session (start practice, end practice, post) and effector (left, right) demonstrated a significant main effect of session, F(2,14) = 18.32, P < 0.01. Post hoc analysis showed that timing variability at the start of practice was higher than at the end of practice and at the post session (P < 0.01 for both). There was also a main effect of effector, F(1,7) = 21.52, P < 0.01, with higher variability scores for the left (0.055 ± 0.013) than right finger (0.050 ± 0.011).
EEG coherence
One-way ANOVA’s on session (start practice, end practice, post) for the different connectivity groupings revealed distinct observations (Fig. 2b). In particular, no significant effect was observed for intrahemispheric left or right, or midline connectivity, P > 0.05. In contrast, the ANOVA for interhemispheric connectivity was significant, F(2,14) = 5.41, P < 0.05. Post hoc analysis indicated increased coherence during the end and post sessions as compared to the start session (P < 0.05 for both).
EEG power
Correlation analyses between the coherence scores of the interhemispheric couplings and the power scores of the individual electrodes showed no significant effects, P > 0.05. The mean correlation coefficients for start, end and post trials were 0.05, 0.16 and 0.18, respectively.
Probing of the in-phase task during pre and post sessions
Behavioral timing
Accuracy and variability scores were estimated. Accuracy: The paired t test on session (pre, post) revealed no significant effect, P > 0.05. The mean scores were 0.007 ± 0.001 and 0.010 ± 0.003 for pre and post trials, respectively. Variability: A two-way ANOVA on session (pre, post) and effector (left, right) showed no significant effects, P > 0.05. The mean scores were 0.036 ± 0.007 and 0.038 ± 0.008 for pre and post trials, respectively.
EEG coherence
The t tests on session (pre, post) for the different connectivity groupings indicated no significant effects, P > 0.05 (Fig. 3a).
Probing of the anti-phase task during pre and post sessions
Behavioral timing
Accuracy and variability scores were calculated. Accuracy: The paired t test on session (pre, post) demonstrated no significant effect, P > 0.05. The mean scores were 0.012 ± 0.002 and 0.014 ± 0.004 for pre and post trials, respectively. Variability: A two-way ANOVA on session (pre, post) and effector (left, right) revealed no significant effects, P > 0.05. The mean scores were 0.040 ± 0.09 and 0.041 ± 0.012 for pre and post trials, respectively.
EEG coherence
The t tests on session (pre, post) for the various connectivity groupings showed divergent results (Fig. 3b). In particular, no significant effect was observed for intrahemispheric left or right connectivity, P > 0.05. In contrast, significance was noted for interhemispheric [t(7) = 3.36, P < 0.05], and for midline connectivity [t(7) = 2.42, P < 0.05], suggesting increased coherence in the post than pre session.
EEG power
Correlation analyses between the coherence scores of the interhemispheric–midline couplings and the power scores of the individual electrodes showed no significant effects, P > 0.05. The mean pre–post scores were 0.08, 0.21 for interhemispheric, 0.02 and −0.12 for midline connectivity.
Group 2: continuous practice of the 2:1 task
This group received continuous practice of the 2:1 pattern (n = 14 trials) to optimize motor practice (Fig. 1).
Practice of the 2:1 task
Behavioral timing
Accuracy and variability scores were determined (Fig. 4a). Accuracy: A one-way ANOVA on session (start practice, end practice, post) illustrated a significant effect, F(2,14) = 7.56, P < 0.01. Post hoc analysis showed that timing accuracy improved from start to end and post sessions (P < 0.05 for both). Variability: A two-way ANOVA on session (start practice, end practice, post) and effector (left, right) indicated a significant main effect of session, F(2,14) = 37.74, P < 0.01. Post hoc analysis revealed that timing variability at the start of practice was higher than at the end of practice and at the post session (P < 0.01 for both). There was also a main effect of effector, F(1,7) = 18.54, P < 0.01, with higher variability scores for the left (0.052 ± 0.010) than right finger (0.048 ± 0.012).
EEG coherence
One-way ANOVA’s on session (start practice, end practice, post) for the different connectivity groupings pointed to similar observations with reduced coherence due to practice (Fig. 4b). In particular, significance effects were noted for intrahemispheric left [F(2,14) = 5.15, P < 0.05], intrahemispheric right [F(2,14) = 4.46, P < 0.05], interhemispheric [F(2,14) = 5.51, P < 0.05], and for midline connectivity [F(2,14) = 5.49, P < 0.05]. Post hoc analyses indicated higher coherence scores during start than end and post sessions (P < 0.05 for all).
EEG power
Correlation analyses between the coherence scores of the intrahemispheric left–right, interhemispheric and midline couplings to the power scores of the individual electrodes showed no significant effects, P > 0.05. The mean scores for start, end and post trials were −0.24, 0.06 and 0.03 for intrahemispheric left, −0.10, −0.23 and −0.14 for intrahemispheric right, −0.22, 0.17 and 0.13 for interhemispheric, −0.20, 0.11 and 0.15 for midline connectivity.
Probing of the in-phase task during pre and post sessions
Behavioral timing
Accuracy and variability scores were estimated. Accuracy: The t test on session (pre, post) exposed no significant effect, P > 0.05. The mean scores were 0.009 ± 0.002 and 0.011 ± 0.003 for pre and post trials, respectively. Variability: A two-way ANOVA on session (pre, post) and effector (left, right) showed no significant effects, P > 0.05. The mean scores were 0.034 ± 0.009 and 0.037 ± 0.007 for pre and post trials, respectively.
EEG coherence
The t tests on session (pre, post) for the various connectivity groupings indicated distinctive results (Fig. 5a). In particular, no significant effect was observed for intrahemispheric left or right, or midline connectivity, P > 0.05. Conversely, significance of the t-test was noted for interhemispheric connectivity with higher coherence in the post than pre session, t(7) = 2.47, P < 0.05.
EEG power
Correlation analyses between the coherence scores of the interhemispheric couplings and the power scores of the individual electrodes showed no significant effects, P > 0.05. The mean pre–post scores were 0.07 and 0.12.
Probing of the anti-phase task during pre and post sessions
Behavioral timing
Accuracy and variability scores were calculated. Accuracy: The t test on session (pre, post) showed no significant effect, P > 0.05. The mean scores were 0.014 ± 0.003 and 0.013 ± 0.004 for pre and post trials, respectively. Variability: A two-way ANOVA on session (pre, post) and effector (left, right) showed no significant effects, P > 0.05. The mean scores were 0.039 ± 0.011 and 0.040 ± 0.010 for pre and post trials, respectively.
EEG coherence
The t tests on session (pre, post) for the different connectivity groupings showed differential outcomes (Fig. 5b). In particular, no significant effect was noted for intrahemispheric left or right connectivity, P > 0.05. Conversely, there was a significant effect for interhemispheric [t(7) = 2.46, P < 0.05], and for midline connectivity [t(7) = 3.15, P < 0.05], pointing to increased coherence in the post than pre session.
EEG power
Correlation analyses between the coherence scores of the interhemispheric–midline couplings and the power scores of the individual electrodes showed no significant effects, P > 0.05. The mean pre–post scores were 0.10, −0.09 for interhemispheric, 0.19 and −0.11 for midline connectivity.
Group comparison of the 2:1 task
Analyses were conducted on practice trial 10 of the 2:1 task, which involved trial 14 for group 1 (interrupted practice) and trial 10 for group 2 (continuous practice). In addition, a group comparison was made for the start practice trial.
Behavioral timing
Accuracy and variability scores were determined. Accuracy: The independent t test (group 1, group 2) on practice trial 10 revealed a significant effect [t(14) = 2.70, P < 0.05], with group 1 being less accurate than group 2. The mean scores were 0.017 ± 0.005 and 0.013 ± 0.003 for group 1 and 2, respectively. The start practice trial from both groups was not significantly different from one another, P > 0.05. Expressed as percentage scores, the data suggested that practice improved temporal accuracy with 19% for group 1 and with 35% for group 2. Variability: A two-way ANOVA on group (group 1, group 2) and effector (left, right) indicated a significant main effect of group, F(1,14) = 21.40, P < 0.01. The mean scores were 0.053 ± 0.015 and 0.047 ± 0.013 for group 1 and 2, respectively. In percentage scores, the observations implied that practice improved temporal variability with 11% for group 1 and with 23% for group 2. The main effect of effector was also significant, F(1,7) = 5.76, P < 0.05, with higher variability scores for the left (0.051 ± 0.014) than right finger (0.049 ± 0.010).
EEG coherence
The independent t tests (group 1, group 2) for the different connectivity couplings on practice trial 10 demonstrated significant effects for all analyses, with group 1 having higher coherences than group 2 (Fig. 6). In particular, significant effects were noted for intrahemispheric left [t(14) = 2.22, P < 0.05], intrahemispheric right [t(14) = 2.34, P < 0.05], interhemispheric [t(14) = 2.95, P < 0.01], and for midline connectivity [t(14) = 2.17, P < 0.05]. The start practice trial of both groups (illustrated in Figs. 2b, 4b) showed no significant effects for intrahemispheric left [t(14) = 0.61, P > 0.05], intrahemispheric right [t(14) = 0.09, P > 0.05], interhemispheric [t(14) = 0.72, P > 0.05], or midline connectivity [t(14) = 0.23, P > 0.05].
Discussion
An important constraint during interlimb coordination is the tendency toward spatiotemporal coupling, which is observed during discrete as well as rhythmic movements (e.g., Kelso et al. 1979, 1981). With respect to rhythmical patterns, this preference manifests itself through a 1:1 frequency ratio that is performed according to an in-phase (symmetrical) or anti-phase (asymmetrical) mode. It consequently reflects pre-existing behavior that can be efficiently executed without any training. Conversely, complex actions that involve polyrhythms or unfamiliar phase relations require practice and are susceptible to interference from the preferred modes (Serrien and Swinnen 1997; Zanone and Kelso 1992). With training, competitive influences from the intrinsic modes disappear during which the new task gains in accuracy and stability. In the present study, training of a motor task with a 2:1 frequency ratio was examined under continuous vs. interrupted practice condition. It was argued that the arrangement of the practice scheme would affect the learning potential due to interactions between new and pre-existing dynamics. Here, motor practice was assessed during the fast learning stage during which significant performance improvements are noticeable within a training session.
The 2:1 task: new motor acquisition and the effect of continuous versus interrupted practice
Bimanual tapping according to a 2:1 ratio implies a simple metrical organization with an explicit representation of temporal goals (Semjen 2002). Due to the particular task demands, the 2:1 arrangement supports a strategy that allows the effectors to move simultaneously for one tap where after the fast effector continues to tap once while the slow effector pauses at peak extension. Therefore, in-phase movements act as an attractor as well as a distractor for the 2:1 task requirements. Accordingly, the hypothesis was made that in-phase trials introduced during a 2:1 training schedule would disturb the normal acquisition process.
The results from the continuous practice group revealed improved behavioral performance along with reduced functional connectivity across the designated network, which underlines that the short training period associated with an enhanced motor experience. This observation denotes that practice-driven plasticity results in efficient inter-regional communication. With interrupted practice, the subjects’ behavioral output improved, although it was inferior compared to that from continuous practice with the 2:1 task, indicating that the in-phase mode interfered with the refinement of the new assignment. In terms of neural activity, there was unchanged intrahemispheric and midline connectivity in addition to increased interhemispheric connectivity. This mixed pattern implies that the interrupted training scheme modified the functional couplings in specific ways. Especially, the augmented coherence is of particular interest and underscores the necessity of additional information processing, including attentional focusing (Johansen-Berg and Matthews 2002; Rowe et al. 2002), for supporting bimanual behavior under challenging conditions. It should be mentioned, however, that the training schedule of the interrupted practice group also implicated the succession of two tasks in close temporal proximity. Accordingly, an inability to switch efficiently between motor acts might have contributed to the observations.
The findings from the interrupted practice group illustrate an impact of one task performance on another, with the in-phase mode having a degrading influence on the practiced 2:1 mode; an effect that was present at the end of training and at retention. The latter observation appears at variance with behavioral data that have shown that random practice (during which trials of tasks are interleaved) provide superior performance at retention as compared to blocked practice (during which trials of tasks are executed separately) (Lee and Magill 1985; Shea and Morgan 1979). This effect of contextual interference implies that practice conditions that involve a relatively high degree of interference due to a variety of motor experiences will benefit learning as observed in retention or transfer conditions (Maslovat et al. 2004). Conversely, the current findings support specificity of learning during which skills are trained according to sensorimotor context (Proteau et al. 1992). In the present paradigm, the effect of practice specificity is likely due to the particular combination of new alongside pre-existing tasks, and was additionally confirmed from the reduced performance of the interrupted versus continuous practice group on an equal number of training trials. This finding underlines that pre-existing dynamics interferes, at least initially, with the progress of new compound actions.
Probing the in-phase and anti-phase pattern before and after 2:1 training
Isofrequency coordination according to an in-phase or anti-phase mode refers to intrinsic behavior. Due to its more complex (asymmetrical) task demands, the anti-phase pattern is usually less successfully performed than the in-phase pattern. Overall, the relative simplicity of the in-phase mode guarantees stable motor performance under normal and perturbed conditions (Fink et al. 1999; Sadato et al. 1997; Serrien and Brown 2002). In the present study, the pre-existing modes were evaluated in order to find out whether new training modified their output and regulation. The results showed that behavioral performance was not affected. However, neural activation patterns were distinctly influenced, depending on training schedule and coordination mode. Of interest was that specific connectivity profiles showed increased activation, which likely reflected compensatory processes at the network level to maintain behavioral output.
For continuous practice, during which in-phase and anti-phase movements were performed before and after 2:1 training, interhemispheric connectivity (for in-phase and anti-phase) and midline connectivity (for anti-phase) increased from pre to post trials, pointing to augmented information processing due to new acquisition. This observation highlights a stronger perturbing effect on the anti-phase than in-phase mode, which underlines the robustness of the latter as compared to former configuration. For interrupted practice, the in-phase mode, which was intermediately performed during 2:1 training, maintained its degree of functional couplings. Conversely, the anti-phase mode, performed only at pre and post trials, showed increased interhemispheric and midline coherence due to new practice. Together, these findings extend fMRI data (Rémy et al. 2008) by detailing the functional connectivity pathways of the motor network that are responsive to new acquisition.
The combined results from the practiced 2:1 and intrinsic 1:1 modes indicate that both influence one another, depending on practice scheme and task characteristics. According to Zanone and Kelso (1992), competition between new and pre-existing behavior shapes skill acquisition, suggesting bidirectional influences between both dynamics. This principle of competition is partly similar to the concepts of retrograde interference (influence of new on previous learning) and anterograde interference (influence of previous on new learning) when consolidating newly acquired skills (Brashers-Krug et al. 1996; Krakauer et al. 2005). Noteworthy is that the influence from pre-existing to new dynamics appeared more powerful than vice versa. This became evident from the combined practice conditions that showed behavioral in addition to neural changes for the 2:1 assignment, whereas only neural adaptations emerged for the 1:1 tasks.
Significance of interhemispheric and midline connectivity during bimanual coordination
Relevant in the present study was to identify the functional couplings that are most responsive to practice of bimanual skill. The data indicated that interhemispheric and midline couplings were most affected while intrahemispheric couplings were less involved. This finding underlines that bilaterally delegated interactions are particularly important in coordinating the processing demands when exigencies on the motor system increase. First, with respect to interhemispheric connectivity, it is acknowledged that it undertakes a decisive influence in bimanual behavior (Grefkes et al. 2008), which may associate with computational complexity or information transfer/suppression between both hemispheres (Belger and Banich 1998; Duque et al. 2005; Kinsbourne 1970; Nowicka et al. 1996). Second, it is recognized that medial areas (including SMA) are crucial for coordinated behavior (Grefkes et al. 2008), which may relate to demands of complexity, inhibition and timing, or subjective task experience (e.g., Chen et al. 1995; Erdler et al. 2001; Macar and Vidal 2002; Sadato et al. 1997; Serrien et al. 2002). Of note is that the significance of interhemispheric and midline areas in challenging conditions may also be due to their ability to modulate each others’ activity (Grefkes et al. 2008; Serrien et al. 2002; Stancák et al. 2003).
Conclusion
Motor skills are generally learned through practice. This progression not only involves acquisition of the particular task demands but also requires overcoming pre-existing modes. By evaluating different training schedules, the present study showed bidirectional influences between both task dynamics, with a stronger impact from pre-existing to new behavior than vice versa. These results propose that the particular arrangement of new and intrinsic tasks during training affects optimization of motor learning, which may have significant implications for scheduling practice and behavioral interventions during neurorehabilitation.
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Acknowledgments
This research was supported by the Biotechnology and Biological Sciences Research Council (Grant BB/F012454/1) and Research Committee (NRF) of the University of Nottingham. Thanks to E. Georgiadi for assistance.
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Serrien, D.J. Interactions between new and pre-existing dynamics in bimanual movement control. Exp Brain Res 197, 269–278 (2009). https://doi.org/10.1007/s00221-009-1910-6
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DOI: https://doi.org/10.1007/s00221-009-1910-6