Abstract
Chromate resistance inRalstonia metallidurans CH34 is based on chromate efflux catalyzed by ChrA efflux pumps. The bacterium harbors two chromate resistance determinants, the previously knownchr 1 on plasmid pMOL28 (geneschrI, chrB 1,chrA 1,chrC, chrE, chrF 1) andchr 2 on the chromosome (geneschrB 2,chrA 2,chrF 2). Deletion of the geneschrl, chrC, chrA 2,chrB 2 andchrF 2 influenced chromate resistance and transcription from achrBp 1::lacZ fusion. Deletion of the plasmid-encoded genechrBx did not change chromate resistance orchrBp 1 regulation. Northern hybridization and primer-extension experiments were used to study transcription of the plasmid-encodedchr 1 determinant. Transcription ofchrB 1,chrA 1 andchrC was induced by chromate. The presence of sulfate influenced transcription positively. ThechrBp 1,chrAp 1 andchrCp promoters showed some similarity to heat-shock promoters. Transcription of the generpoH encoding a putative heat-shock sigma factor was also induced by chromate, butrpoH was not essential for chromate resistance. The ChrC protein was purified as a homotetramer and exerted superoxide dismutase activity. Thus, possible regulators for chromate resistance (Chrl, ChrB1, ChrB2, ChrF1, and ChrF2) and an additional detoxification system (ChrC) were newly identified as parts of chromate resistance inR. metallidurans.
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Abbreviations
- SOD :
-
Superoxide dismutase
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Electronic supplementary material to this paper can be obtained by using the Springer LINK server located at http://dx.d0i.0r2/>10.1007/s00203-002-0492-5.
Published online: 7 November 2002
An erratum to this article is available at http://dx.doi.org/10.1007/s00203-004-0665-5.
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Juhnke, S., Peitzsch, N., Hübener, N. et al. New genes involved in chromate resistance inRalstonia metallidurans strain CH34. Arch Microbiol 179, 15–25 (2002). https://doi.org/10.1007/s00203-002-0492-5
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DOI: https://doi.org/10.1007/s00203-002-0492-5