Abstract
Purpose
Necrotising soft tissue infection (NSTI) is a deadly disease associated with a significant risk of mortality and long-term disability from limb and tissue loss. The aim of this study was to determine the effect of hyperbaric oxygen (HBO2) therapy on mortality, complication rate, discharge status/location, hospital length of stay and inflation-adjusted hospitalisation cost in patients with NSTI.
Methods
This was a retrospective study of 45,913 patients in the Nationwide Inpatient Sample (NIS) from 1988 to 2009.
Results
A total of 405 patients received HBO2 therapy. The patients with NSTI who received HBO2 therapy had a lower mortality (4.5 vs. 9.4 %, p = 0.001). After adjusting for predictors and confounders, patients who received HBO2 therapy had a statistically significantly lower risk of dying (odds ratio (OR) 0.49, 95 % confidence interval (CI) 0.29–0.83), higher hospitalisation cost (US$52,205 vs. US$45,464, p = 0.02) and longer length of stay (LOS) (14.3 days vs. 10.7 days, p < 0.001).
Conclusions
This retrospective analysis of HBO2 therapy in NSTI showed that despite the higher hospitalisation cost and longer length of stay, the statistically significant reduction in mortality supports the use of HBO2 therapy in NSTI.
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Introduction
Necrotising soft tissue infections (NSTI) are associated with mortality rates ranging from 0 to 73 % [1–3]. In addition, they can lead to significant morbidity such as limb amputations. Early surgical debridement and antibiotics remain the mainstay of management, but hyperbaric oxygen therapy (HBO2 therapy) has been used as an adjunct. Although there are many uncontrolled case series [4] and expert opinions about the role of HBO2 therapy in NSTI, as far as we know, no controlled studies have been conducted with a sample size greater than 80 [5]. Published articles have repeatedly stated that there is a lack of evidence for the effectiveness of HBO2 therapy in NSTI and thus it is not uncommon to read about statements describing the lack of adequately powered or controlled trials in HBO2 therapy for NSTI [4, 6, 7]. The rarity of these conditions [8] and their high acuity make prospective randomised studies difficult to perform, and these are unlikely ever to occur. A recent review stated that the weakness of previous studies precludes a meta-analysis to resolve contradictory findings and a firm recommendation on the use of HBO2 therapy for NSTI cannot be made until better designed studies are conducted [7]. It is to address exactly this point that the current study was performed.
Materials and methods
Methods
Ethics approval was obtained from the National University of Singapore’s institutional review board. The study involved secondary analysis of the dataset in the Nationwide Inpatient Sample (NIS) database which avoids any linkage to individual patients and effectively anonymizes the patients captured in the dataset. The NIS is an all-payer inpatient care database that is part of the Healthcare Cost and Utilisation Project (HCUP) of the Agency for Healthcare Research and Quality in the USA. It captures information such as mortality, charges and hospital length of stay (LOS) from 1,044 hospitals in 40 states in the USA. The database reflects a 20 % stratified sample of community hospitals. On the basis of the American Hospital Association definition of a community hospital, this includes public hospitals and academic medical centres. It does not include federal hospitals (mostly veterans administration hospitals) or hospital units of institutions such as prisons and infirmaries. The NIS database effectively incorporates 90 % of all hospital discharges in the USA between 1988 and 2009. It contains data on more than 7,000,000 hospital stays which makes it the largest database of its kind in the USA. It has been used to track healthcare trends and also provides a valuable resource for research into uncommon diseases. A maximum of 15 diagnoses and 15 procedures can be captured in its data set. However the actual number recorded varies from state to state. This study compared the mortality, complications, hospital LOS, hospitalisation cost, discharge status and location of patients with NSTI who received HBO2 therapy versus those who did not. We performed a retrospective study on patients in the NIS database from 1988 to 2009. The templates and material developed by the Research on Research group, for formulating a research question (Question Diagram) and writing the manuscript, while conducting this secondary data analysis/study were used [9, 10]. All patients in the NIS database who have NSTI were included in the study. Patients were identified using the International Classification of Diseases, Ninth Revision, Clinical Modification (ICD-9-CM) codes. The ICD-9-CM is derived from the World Health Organisation’s Ninth Revision of the International Classification of Diseases (ICD-9). Patients meeting the inclusion criteria were selected using ICD-9-CM codes for necrotising fasciitis, Fournier’s and gas gangrene. Patients who received HBO2 therapy formed one group and those who did not receive HBO2 therapy formed the control group.
Outcomes
In-hospital mortality was the primary outcome. Secondary outcomes included the disposition of the patient (discharge status and location), hospital LOS, total inflation-adjusted hospital charges, complications related to NSTI including limb loss (amputations), tissue loss (revision of amputation stump), cardiovascular collapse, cardiac arrest, cardiac complications (cardiac arrest or failure following anaesthesia), acute myocardial infarction, shock, respiratory arrest, pulmonary embolism, pneumonia, cerebrovascular accidents (CVA), severe sepsis (sepsis with acute organ dysfunction, sepsis with multiple organ dysfunction (MOD), systemic inflammatory response syndrome due to infectious process with acute organ dysfunction) and acute organ dysfunction (acute renal failure, acute and chronic respiratory failure, critical illness myopathy/polyneuropathy, disseminated intravascular coagulation, metabolic encephalopathy, acute hepatic failure, septic shock, heart failure), complications related to HBO2 therapy including ear barotrauma, seizures, pneumothoraces, and hypoglycemia. The discharge can be routine or non-routine. A non-routine discharge includes discharge to a short-term hospital, skilled nursing facility, intermediate care facility, another type of facility, home health care, against medical advice or death. The hospitalisation cost was adjusted for inflation by multiplying it by the inflation factor for the corresponding year. Complications were identified by searching all diagnosis and procedure fields for the ICD-9-CM codes.
Predictors and confounders
The ICD-9-CM procedure codes for hyperbaric oxygenation and decompression chamber were used to identify the patients in the HBO2 therapy group; all the other patients were assigned to the non-HBO2 therapy group.
Possible confounders included age, gender, the patient’s county of residence, hospital characteristics (bed size, location and teaching status), and the Deyo clinical co-morbidity index. This index is a weighting of various conditions including myocardial infarction, congestive heart failure, peripheral vascular disease, cerebrovascular disease, dementia, chronic pulmonary disease, rheumatologic disease, peptic ulcer disease, mild liver disease, diabetes, diabetes with chronic complications, hemiplegia or paraplegia, renal disease, any malignancy, including leukaemia and lymphoma, moderate or severe liver disease, metastatic solid tumour and AIDS [11]. These co-morbid conditions were identified using the ICD-9-CM diagnosis and procedure codes.
The patients were stratified according to the key confounders such as the admission source, admission type, site of infection and aetiology of infection. Patients were stratified to truncal/extremity NSTI, traumatic/spontaneous NSTI, clostridial myonecrosis/non-clostridial NSTI, admission source and admission type.
Patients with truncal NSTI were identified by searching all the procedure fields in the NIS database for the ICD-9-CM procedure codes that correspond to operations in the truncal region. Similarly, patients with extremity NSTI were identified by searching for the procedure codes corresponding to limb amputations. Patients with trauma-related NSTI were identified by searching for the ICD-9-CM diagnosis codes that correspond to trauma. All other patients were grouped into the non-traumatic NSTI group.
Patients with clostridial myonecrosis were identified by searching for the ICD-9-CM diagnosis code that corresponds to gas gangrene. Patients with non-clostridial myonecrosis were identified using the codes for necrotising fasciitis and Fournier’s gangrene.
Statistical analysis
The two-sample t test was used to compare the ages of the HBO2 therapy and non-HBO2 therapy groups. The two-sample Wilcoxon rank-sum (Mann–Whitney) test was used to compare the LOS and total inflation-adjusted hospital charges. The Pearson chi-squared test was used to test association between the confounders (gender, discharge, hospital size, type of hospital, Deyo co-morbidity index, admission type, admission source, site of NSTI, aetiology of NSTI), the outcomes (mortality, amputations, need for revision of stump, complications) and predictor (HBO2 therapy and non-HBO2 therapy group). Multivariate logistic regression was used to adjust for known confounders when calculating the odds ratio (OR). Quantile regression was used to determine the adjusted medians for the LOS and inflation-adjusted hospital charges. In order to reduce potential bias due to an imbalance in patient characteristics between the HBO2 therapy and non-HBO2 therapy groups, a propensity score [12] was derived using a logistic regression for predictor (HBO2 therapy vs. non-HBO2 therapy) as the dependent variable and the aforementioned set of confounders as the independent variables. All the multivariate logistic and quantile regressions for the outcome variables were adjusted for this propensity score. A p value less than 0.05 was considered to be statistically significant. Data from patients with missing values were not analysed. The data were analysed using the Stata/SE 9.2 (Stata Corp., College Station, TX, USA) statistical software.
Results
A total of 45,913 patients were diagnosed with NSTI in the NIS database, of whom 405 (0.88 %) received HBO2 therapy. The demographics and disposition of the patients are presented in Table 1. The patients who received HBO2 therapy had more co-morbidities based on the Deyo scoring system. In addition, these patients were more likely to be in a larger, urban hospital. Fewer of the patients who received HBO2 therapy were emergency admissions. There was no statistically significant difference in the anatomical site or the probability that the disease was trauma-related.
Patients who received HBO2 therapy had a lower mortality and complications related to NSTI (Table 2). However they were more likely to have a longer LOS in the hospital and higher cost for their hospitalisations. None of the patients who received HBO2 therapy were reported to have complications associated with HBO2 therapy (ear barotrauma, seizures, pneumothorax, hypoglycemia). Using the propensity score as a balancing score, patients who received HBO2 therapy had a lower mortality (OR 0.49, 95 % confidence interval (CI) 0.29–0.83, p = 0.008). Both groups were equally likely to have a routine discharge and to develop complications related to NSTI. The adjusted median LOS was 14.3 days (95 % CI 13–16) for the patients who received HBO2 therapy versus 10.7 days (95 % CI 10–11) for the patients who did not receive HBO2 therapy (p < 0.001). A subgroup analysis of patients who survived to hospital discharge was performed and the adjusted median LOS for the survivors was 14.1 days (95 % CI 12.9–15.4) for those who received HBO2 therapy versus 10.7 days (95 % CI 10.6–10.9) for those who did not receive HBO2 therapy (p < 0.001). However, for the patients who died in hospital, the adjusted median duration of survival was 17.1 days (95 % CI 9.7–24.4) for those who received HBO2 therapy versus 8.8 days (95 % CI 8.2–9.3) for those who did not receive HBO2 therapy (p = 0.03). The adjusted median cost was US$52,205 (95 % CI US$46,397–58,012) for those who received HBO2 therapy versus US$45,464 (95 % CI US$44,867–46,060) for those who did not receive HBO2 therapy (p < 0.001). Tables 3 and 4 show the outcomes after stratification.
Discussion
There was no difference in the rate of complications in the two groups following adjustment using the propensity score. Patients in the HBO2 therapy group had a longer adjusted median hospital LOS and a higher adjusted median hospitalisation cost. The longer LOS among the subgroup of survivors in the HBO2 therapy group compared to the control group demonstrates that this could not be attributed to a difference in survival duration. Another study reported higher average daily costs for patients receiving HBO2 therapy although there was no statistically significant difference in total costs [8]. The difference in adjusted median cost in our study is US$6,741, probably due largely to the cost of HBO2 therapy and the longer LOS in the HBO2 therapy group. It is not possible to determine how much of the additional cost is attributable to HBO2 therapy as the NIS database does not capture the number of HBO2 therapy sessions.
In this study, patients with clostridial myonecrosis did not seem to benefit from HBO2 therapy which is not consistent with the findings in previous studies [13]. A possible explanation may be that for this subset, the study is underpowered: only 66 of the 10,098 patients with clostridial myonecrosis received HBO2 therapy in the database. Patients with non-clostridial myonecrosis who received HBO2 therapy had a lower mortality. However there was a higher hospitalisation cost and longer LOS. Other studies have also reported improved outcomes with HBO2 therapy in non-clostridial myonecrosis [14]. Despite the size of this study it is still underpowered to test the effect of HBO2 therapy in specific subgroups such as extremity versus truncal infection.
There are many animal studies suggesting possible mechanisms of benefit of HBO2 therapy in NSTI. Proposed mechanisms include improved oxygenation [15, 16], inhibition of anaerobic infections [17], oedema reduction [18], enhanced white cell action [19–24], elevation of superoxide dismutase levels [25], reducing systemic inflammation [26], increased effectiveness of antimicrobial agents [27], inhibition of alpha-toxin production [28] and growth of clostridial organisms [29]. During later stages, HBO2 therapy improves angiogenesis [30], fibroblast activity and collagen synthesis [31], thereby promoting wound healing.
There are many studies supporting the use of HBO2 therapy in NSTI [13, 14, 32]. However, there are also studies which have not shown any survival benefit [4, 5, 33]. One possible explanation is that these earlier studies may not have been adequately powered [6]. There are few published collective reviews and these mostly contain insufficient detail to do a retrospective case–control study. In addition, the number of HBO2 therapy sessions may have been too few for it be beneficial [33]. The failure to adjust for significant predictors and confounders in many of these studies could also have accounted for the negative results [4].
Limitations of the study
Although we may not have included every possible complication in the analysis, it is unlikely that the rarer complications would have affected the result. The NIS database does not capture several predictors and confounders for the outcome of patients with NSTI. This includes the time to diagnosis [34], performance of a tissue biopsy (which could speed up the diagnosis [35], time to surgical debridement [4, 36], number and adequacy of surgical debridement [37], body surface area involved/extent of the NSTI [4], hyperlactataemia [4], number of HBO2 therapy sessions, immune globulin use [38], antibiotic use, physiologic parameters and diseases on admission (white blood cell count, haematocrit, creatinine levels, heart disease and the presence of shock), multiple-organ dysfunction syndrome (MODS) scores, Acute Physiology and Chronic Health Evaluation (APACHE) II scores [39] and whether they were ICU admissions or not. Thus it is not possible to analyse the effect of these confounders on the outcomes. The lack of data regarding the number of HBO2 therapy sessions makes it impossible to estimate a dose–response which could strengthen the notion of a true treatment effect. On the other hand, despite the lack of consensus about the optimal number of treatment sessions for NSTI [6], most HBO2 therapy treatments for NSTI are short-term in nature, averaging 3–10 sessions [14, 32]. Thus this may not be a significant confounding factor. As the NIS database only captures hospital mortality, there is no information available on patients who die following hospital discharge. This may explain the lower mortality rates in the current study as compared to studies with longer follow-up [8]. Another possible limitation is the accuracy of the coding for the various diagnoses. This could lead to failure to include patients into the analysis which will affect the results. Nevertheless, the NIS database still provides the largest sample of patients with NSTI undergoing HBO2 therapy. It is conceivable that selection bias may have occurred as a result of varying availability of HBO2 therapy in different types of treatment facilities (e.g. community hospitals vs. urban medical centres) and varying severity of illness of the NSTI (e.g. emergency, urgent admission vs. elective). However, these were identified as possible confounders and included in the propensity score. Although HBO2 therapy has been shown to be safe in critically ill patients [40], it is possible that this was withheld from some patients who were deemed too sick to withstand the therapy (e.g. patients with shock, mechanically ventilated patients). We were unable to test for this as the NIS database does not distinguish between shock preceding the initiation of HBO2 therapy or shock that occurs after HBO2 therapy has commenced. Indeed, APACHE scores are not recorded in the NIS database, thus it is not possible to address that question directly. However we feel that possibility is unlikely, since acute complications did not differ between groups. Moreover, our own practice is usually to keep sick patients ventilated between operative debridements and during HBO2 therapy even when they could be extubated, in order to optimise oxygenation and tissue oxygen delivery.
Summary
To our knowledge, this is the largest study of the effects of HBO2 therapy on patients with NSTI. Our study shows that patients with NSTI who received HBO2 therapy had a lower mortality compared to patients who did not. The lower mortality was observed in the HBO2 therapy group despite the fact that these patients were older and had a higher Deyo co-morbidity index. Patients who received HBO2 therapy had a statistically significantly lower adjusted risk of in-hospital death. The results of this study add to the increasing body of evidence supporting the use of HBO2 therapy in patients with NSTI. Certainly, there was no suggestion of harm from the use of HBO2 therapy. To address this question more definitively a multi-centre randomised controlled trial may be required. However, this may prove to be difficult given the small number of patients with this condition in individual institutions [8]. In addition, given the strength of evidence supporting the use of HBO2 therapy combined with antibiotics and surgery in clostridial myonecrosis and its high mortality, it has been suggested that where HBO2 therapy is available it would be unethical to conduct a randomised controlled trial for this disease [14]. We conclude that even when taking into account the additional LOS and hospitalisation costs, the reduction in mortality associated with HBO2 therapy justifies its use in NSTI.
References
Pizzorno R, Bonini F, Donelli A, Stubinski R, Medica M, Carmignani G (1997) Hyperbaric oxygen therapy in the treatment of Fournier’s disease in 11 male patients. J Urol 158:837–840
Rouse TM, Malangoni MA, Schulte WJ (1982) Necrotizing fasciitis: a preventable disaster. Surgery 92:765–770
Escobar SJ, Slade JB Jr, Hunt TK, Cianci P (2005) Adjuvant hyperbaric oxygen therapy (HBO2) for treatment of necrotizing fasciitis reduces mortality and amputation rate. Undersea Hyperb Med 32:437–443
Elliott DC, Kufera JA, Myers RA (1996) Necrotizing soft tissue infections: risk factors for mortality and strategies for management. Ann Surg 224:672–683
George M, Rueth N, Skarda D, Chipman J, Quickel R, Beilman G (2009) Hyperbaric oxygen does not improve outcome in patients with necrotizing soft tissue infection. Surg Infect (Larchmt) 10:21–28
Jallali N, Withey S, Butler P (2005) Hyperbaric oxygen as adjuvant therapy in the management of necrotizing fasciitis. Am J Surg 189:462–466
Sarani B, Strong M, Pascual J, Schwab CW (2009) Necrotizing fasciitis: current concepts and review of the literature. J Am Coll Surg 208:279–288
Mindrup SR, Kealey GP, Fallon B (2005) Hyperbaric oxygen for the treatment of Fournier’s gangrene. J Urol 173:1975–1977
Pietrobon R, Guller U, Martins H, Menezes AP, Higgins LD, Jacobs DO (2004) A suite of web applications to streamline the interdisciplinary collaboration in secondary data analyses. BMC Med Res Methodol 4:29
Shah J, Shah A, Pietrobon R (2009) Scientific writing of novice researchers: what difficulties and encouragements do they encounter? Acad Med 84:511–516
Deyo R, Cherkin D, Ciol M (1992) Adapting a clinical comorbidity index for use with ICD-9-CM administrative databases. J Clin Epidemiol 45:613–619
Rosenbaum PR, Rubin DB (1983) The central role of the propensity score in observational studies for causal effects. Biometrika 70:41–55
Jackson RW, Waddell JP (1973) Hyperbaric oxygen in the management of clostridial myonecrosis (gas gangrene). Clin Orthop Relat Res 96:271–276
Riseman JA, Zamboni WA, Curtis A, Graham DR, Konrad HR, Ross DS (1990) Hyperbaric oxygen therapy for necrotizing fasciitis reduces mortality and the need for debridements. Surgery 108:847–850
Korhonen K, Kuttila K, Niinikoski J (1999) Subcutaneous tissue oxygen and carbon dioxide tensions during hyperbaric oxygenation: an experimental study in rats. Eur J Surg 165:885–890
Korhonen K (2000) Hyperbaric oxygen therapy in acute necrotizing infections with a special reference to the effects on tissue gas tensions. Ann Chir Gynaecol Suppl:7–36
Brummelkamp W, Hogendyk J, Boerema I (1961) Treatment of anaerobic infections by drenching the tissue with oxygen under high atmospheric pressure. Surgery 49:299–302
Bird AD, Telfer AB (1965) Effect of hyperbaric oxygen on limb circulation. Lancet 1:355–356
Mader JT, Brown GL, Guckian JC, Wells CH, Reinarz JA (1980) A mechanism for the amelioration by hyperbaric oxygen of experimental staphylococcal osteomyelitis in rabbits. J Infect Dis 142:915–922
Mader J, Adams K, Wallace W, Calhoun J (1990) Hyperbaric oxygen as adjunctive therapy for osteomyelitis. Infect Dis Clin North Am 4:433–440
Mandell GL (1974) Bactericidal activity of aerobic and anaerobic polymorphonuclear neutrophils. Infect Immun 9:337–341
Hohn DC, MacKay RD, Halliday B, Hunt TK (1976) Effect of O2 tension on microbicidal function of leukocytes in wounds and in vitro. Surg Forum 27:18–20
Hunt TK, Linsey M, Grislis H, Sonne M, Jawetz E (1975) The effect of differing ambient oxygen tensions on wound infection. Ann Surg 181:35–39
Knighton DR, Halliday B, Hunt TK (1984) Oxygen as an antibiotic: the effect of inspired oxygen on infection. Arch Surg 119:199–204
Kaelin CM, Im MJ, Myers RA, Manson PN, Hoopes JE (1990) The effects of hyperbaric oxygen on free flaps in rats. Arch Surg 125:607–609
Rinaldi B, Cuzzocrea S, Donniacuo M, Capuano A, Di Palma D, Imperatore F, Mazzon E, Di Paola R, Sodano L, Rossi F (2011) Hyperbaric oxygen therapy reduces the toll-like receptor signaling pathway in multiple organ failures. Intensive Care Med 37:1110–1119
Knighton DR, Halliday B, Hunt TK (1986) Oxygen as an antibiotic: a comparison of the effects of inspired oxygen concentration and antibiotic administration on in vivo bacterial clearance. Arch Surg 121:191–195
Unnika V (1965) Inhibition of toxin production in Clostridium perfringens in vitro by hyperbaric oxygen. Antonie Van Leeuwenhoek 31:181–186
Maclennan J, Macfarlane R (1945) Toxin and antitoxin studies of gas gangrene in man. Lancet 246:301–305
Marx RE, Ehler WJ, Tayapongsak P, Pierce LW (1990) Relationship of oxygen dose to angiogenesis induction in irradiated tissue. Am J Surg 160:519–524
Hunt TK, Pai MP (1972) The effect of varying ambient oxygen tensions on wound metabolism and collagen synthesis. Surg Gynecol Obstet 135:561–567
Clark LA, Moon RE (1999) Hyperbaric oxygen in the treatment of life-threatening soft-tissue infections. Respir Care Clin N Am 5:203–219
Brown D, Davis N, Lepawsky M, Cunningham J, Kortbeek J (1994) A multicenter review of the treatment of major truncal necrotizing infections with and without hyperbaric oxygen therapy. Am J Surg 167:485–489
Majeski JA, Alexander JW (1983) Early diagnosis, nutritional support, and immediate extensive debridement improve survival in necrotizing fasciitis. Am J Surg 145:784–787
Stamenkovic I, Lew PD (1984) Early recognition of potentially fatal necrotizing fasciitis: the use of frozen-section biopsy. N Engl J Med 310:1689–1693
Boyer A, Vargas F, Coste F, Saubusse E, Castaing Y, Gbikpi-Benissan G, Hilbert G, Gruson D (2009) Influence of surgical treatment timing on mortality from necrotizing soft tissue infections requiring intensive care management. Intensive Care Med 35:847–853
Freischlag JA, Ajalat G, Busuttil RW (1985) Treatment of necrotizing soft tissue infections: the need for a new approach. Am J Surg 149:751–755
Kaul R, McGeer A, Low DE, Green K, Schwartz B (1997) Population-based surveillance for group A streptococcal necrotizing fasciitis: clinical features, prognostic indicators, and microbiologic analysis of seventy-seven cases. Ontario Group A Streptococcal Study. Am J Med 103:18–24
Mohammedi I, Ceruse P, Duperret S, Vedrinne J, Bouletreau P (1999) Cervical necrotizing fasciitis: 10 years’ experience at a single institution. Intensive Care Med 25:829–834
Weaver LK (2011) Hyperbaric oxygen in the critically ill. Crit Care Med 39:1784–1791
Acknowledgments
We wish to thank Dr. Tam C. Ha (Ph.D., Division of Clinical Trials and Epidemiological Sciences, National Cancer Centre, Singapore) for reading the manuscript and providing her valuable comments. Institutional (financial) support was received from Duke-NUS Graduate Medical School.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Soh, C.R., Pietrobon, R., Freiberger, J.J. et al. Hyperbaric oxygen therapy in necrotising soft tissue infections: a study of patients in the United States Nationwide Inpatient Sample. Intensive Care Med 38, 1143–1151 (2012). https://doi.org/10.1007/s00134-012-2558-4
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00134-012-2558-4