Abstract
Aims/hypothesis
TBC1D1 (tre-2/USP6, BUB2, cdc16 domain family member 1) is a Rab GTPase-activating protein (RabGAP) that has been implicated in regulating GLUT4 trafficking. TBC1D1 can be phosphorylated by the AMP-activated protein kinase (AMPK) on Ser231, which consequently interacts with 14-3-3 proteins. Given the key role for AMPK in regulating insulin-independent muscle glucose uptake, we hypothesised that TBC1D1-Ser231 phosphorylation and/or 14-3-3 binding may mediate AMPK-governed glucose homeostasis.
Methods
Whole-body glucose homeostasis and muscle glucose uptake were assayed in mice bearing a Tbc1d1 Ser231Ala-knockin mutation or harbouring skeletal muscle-specific Ampkα1/α2 (also known as Prkaa1/2) double-knockout mutations in response to an AMPK-activating agent, 5-aminoimidazole-4-carboxamide-1-β-d-ribofuranoside (AICAR). Exercise-induced muscle glucose uptake and exercise capacity were also determined in the Tbc1d1 Ser231Ala-knockin mice.
Results
Skeletal muscle-specific deletion of Ampkα1/a2 in mice prevented AICAR-induced hypoglycaemia and muscle glucose uptake. The Tbc1d1 Ser231Ala-knockin mutation also attenuated the glucose-lowering effect of AICAR in mice. Glucose uptake and cell surface GLUT4 content were significantly lower in muscle isolated from the Tbc1d1 Ser231Ala-knockin mice upon stimulation with a submaximal dose of AICAR. However, this Tbc1d1 Ser231Ala-knockin mutation neither impaired exercise-induced muscle glucose uptake nor affected exercise capacity in mice.
Conclusions/interpretation
TBC1D1-Ser231 phosphorylation and/or 14-3-3 binding partially mediates AMPK-governed glucose homeostasis and muscle glucose uptake in a context-dependent manner.
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Introduction
Type 2 diabetes has become pandemic in the last few decades, which urges novel therapeutics to prevent, treat or cure the disease. As insulin sensitivity is generally decreased in type 2 diabetic patients, insulin-independent treatment is commonly considered to combat type 2 diabetes. The energy sensor AMP-activated protein kinase (AMPK) has been proposed as an attractive drug target for treatment of type 2 diabetes as its activation brings about many of the acute and chronic beneficial effects of exercise and promotes muscle glucose uptake in an insulin-independent mechanism [1, 2].
The heterotrimeric AMPK holoenzyme consists of a catalytic subunit α (α1 and α2), a regulatory subunit γ (γ1, γ2 and γ3) and a third subunit β (β1 and β2) that bridges the α and γ subunits [1]. AMPK regulates whole-body glucose homeostasis by various mechanisms [1, 3, 4], one of which is control of muscle glucose uptake by promoting translocation of GLUT4 from its intracellular storage sites onto plasma membrane [5]. Whole-body deletion of the AMPK α2 or β2 subunit, or overexpression of a dominant inhibitory mutant of AMPK in muscle, attenuates the hypoglycaemic effect of AICAR in mice. Furthermore, genetic inactivation of the AMPK α2, β2 or γ3 subunit, or overexpression of the dominant inhibitory mutant of AMPK, inhibits AICAR-stimulated muscle glucose uptake [6–10]. However, the regulatory mechanism of AMPK-dependent GLUT4 translocation remains to be elucidated. GLUT4 also mediates insulin-stimulated glucose uptake into skeletal muscle and adipose, in which translocation in response to insulin is regulated by the protein kinase B (PKB, also known as Akt) pathway [11].
Two related Rab GTPase-activating proteins (RabGAPs), Akt substrate of 160 kDa (AS160, also known as TBC1D4) and tre-2/USP6, BUB2, cdc16 domain family member 1 (TBC1D1), have been linked with type 2 diabetes and obesity, respectively [12–15]. These enzymes have also been implicated in regulating GLUT4 trafficking (reviewed by Sakamoto and Holman [16]). Both AS160 and TBC1D1 can be phosphorylated on multiple sites by protein kinases including PKB and AMPK (reviewed by Chen et al [17]). Overexpression of an AS160-4P mutant (in which Ser325, Ser595, Thr649 and Ser758 were replaced by alanine) or a TBC1D1-3P mutant (in which alanine replaced Ser489, Thr499/Ser501 and Thr590) robustly inhibits insulin-stimulated GLUT4 translocation in 3T3-L1 adipocytes [18, 19]. In contrast, overexpression of a TBC1D1-4P mutant (in which alanine replaced Ser231, Thr499, Thr590 and Ser621) decreases contraction-induced glucose uptake in mouse skeletal muscle [20]. Upon phosphorylation, both AS160 and TBC1D1 bind to regulatory 14-3-3 proteins (reviewed by Chen et al [17]). The AS160−14-3-3 interaction is regulated by insulin and mainly mediated by phosphorylated Thr649 on AS160 [21, 22], whereas 14-3-3 binding to TBC1D1 depends on AMPK and mainly requires phosphorylated Ser231 on TBC1D1 [23, 24]. Moreover, 14-3-3 binding to AS160 has been implicated in participating insulin-stimulated GLUT4 translocation in 3T3-L1 adipocytes [21]. On the basis of these findings, we put forward a hypothesis that AS160−14-3-3 interaction is required for insulin-stimulated GLUT4 trafficking and glucose homeostasis, while 14-3-3 binding to TBC1D1 mediates AMPK-dependent GLUT4 translocation and glucose homeostasis [17]. Towards addressing this hypothesis, we previously generated an As160 Thr649Ala-knockin mouse model in which insulin-induced AS160-Thr649 phosphorylation and its binding to 14-3-3s was prevented, and showed that the As160 Thr649Ala-knockin mice exhibited insulin resistance mainly through impaired GLUT4 translocation and glucose uptake into skeletal muscle in response to insulin [25]. In contrast, AICAR-stimulated muscle glucose uptake and hypoglycaemia, which are independent of insulin, were normal in these mice [26].
In this study, we used a recently reported Tbc1d1 Ser231Ala-knockin mouse model in which the knockin mutation prevents AMPK-dependent phosphorylation of TBC1D1-Ser231 [27] to further test the above hypothesis.
Methods
Materials
Recombinant human insulin was bought from Novo Nordisk (Bagsvaerd, Denmark) and AICAR from Toronto Research Chemicals (Toronto, ON, Canada). Protein G-Sepharose was from GE Healthcare (Little Chalfont, UK). 2-deoxy-d-[1,2-3H(N)]glucose and d-1-[14C]mannitol were from PerkinElmer (American Fork, UT, USA). All other chemicals were from Sigma-Aldrich (Shanghai, China) or Sangon Biotech (Shanghai, China). Total TBC1D1 antibody is as previously described [23]. The commercial antibodies are listed in electronic supplementary material (ESM) Table 1.
Mouse breeding and genotyping
The Ethics Committees, initially at the University of Dundee and latterly at Nanjing University, approved all animal protocols. Mice were kept under a light/dark cycle of 12 h. Animal experiments were not carried out in a blinded or randomised manner. The Tbc1d1 Ser231Ala-knockin mice were generated at the University of Dundee as previously described [27]. The Ampkα1 f/f and Ampkα2 f/f mice were as previously described [28], and obtained from the Jackson Laboratory (Bar Harbor, ME, USA). They were mated with each other to obtain Ampkα1/α2 f/f mice. The Ampkα1/α2 f/f mice were then mated with the Myf5-Cre mice [29] to obtain the Ampkα1/α2 f/f–Myf5-Cre mice that are the skeletal muscle-specific Ampkα1/α2-knockout (Ampkα1/α2-mKO) mice. Genotyping was carried out using primers listed in ESM Table 2.
Tissue lysis, immunoprecipitation, immunoblotting and 14-3-3 overlay
Homogenisation of mouse tissues and protein concentration measurement were carried out as previously described [26].
Immunoprecipitation of TBC1D1 protein, immunoblotting and 14-3-3 overlay were carried out as previously described [23].
AICAR tolerance and insulin tolerance tests
Mice were partially fasted for 4 h prior to AICAR or insulin tolerance test. Afterwards, basal blood glucose was measured using a Breeze 2 glucometer (Bayer, Leverkusen, Germany) via tail bleeding. Mice were intraperitoneally injected with AICAR (0.25 mg/g) for AICAR tolerance tests or insulin (0.75 mU/g) for insulin tolerance tests. After injection of AICAR or insulin, blood glucose levels were measured at the indicated time intervals.
Muscle incubation, glucose uptake and photolabelling of cell surface GLUT4 in isolated muscle
Muscle isolation and glucose uptake assays were carried out as previously described [25]. Briefly, isolated muscles were incubated in KRB (± AICAR) for 50 min. For biochemistry studies, muscles were then blotted dry and snap frozen in liquid nitrogen for subsequent analysis. For glucose uptake, muscles were further incubated in KRB (± AICAR) containing 2-deoxy-d-[1,2-3H(N)]glucose and d-1-[14C]mannitol for 10 min. Radioisotopes in muscle lysates were determined using a Tri-Carb 2800TR scintillation counter (PerkinElmer). For the photolabelling experiment, cell surface GLUT4 in isolated extensor digitorum longus (EDL) muscle was chemically tagged with the photolabel Bio-LC-ATB-BGPA and quantified as previously described [25].
Treadmill exercise
Two types of treadmill running tests, a power test (short high-intensity run) and an endurance test (long low-intensity run), were performed to assess maximal exercise performance in mice using a treadmill (Techman Soft, Chengdu, China) as previously described [30]. Briefly, mice were acclimated to treadmill running (5 min per day at 15 cm/s, +5° slope and 0.3 mA electrical stimulation) for 5 days before the tests. For power tests, the treadmill was set with a +5° slope and 0.3 mA aversive electrical stimulation. Belt speed was set at 15 cm/s initially and increased by 2 cm/s every minute. As for endurance tests, the treadmill was set with a +5° slope and 0.3 mA aversive electrical stimulation. Belt speed was set at 15 cm/s initially and increased by 3 cm/s every 12 min. Running tests were terminated when mice failed to re-engage on the treadmill despite aversive stimulation for more than 15 s.
Exercise-stimulated muscle glucose uptake in vivo
Exercise-stimulated muscle glucose uptake was carried out as previously described [31]. Briefly, mice were intraperitoneally injected with 3.7 × 105 Becquerel (Bq) of 2-deoxy-d-[1,2-3H(N)]glucose immediately prior to rest or exercise. For the exercise group, mice were subjected to 35 min of treadmill running (30 cm/s belt speed, +10° slope). For the resting group, mice were kept in home cages for 35 min. After exercise or rest, mice were euthanised and gastrocnemius and quadriceps muscles were harvested for further analysis. An assumption was made that systemic delivery of the tracer was similar in all animals within each experimental group as the appearance of 2-deoxy-d-[1,2-3H(N)]glucose in the blood was not determined for technical reasons. Muscle accumulation of phosphorylated 2-deoxy-d-[1,2-3H(N)]glucose was determined and used for calculation of in vivo muscle glucose-uptake rates as previously described [25].
Histology and imaging
Isolated soleus and EDL muscles were embedded in tissue freezing medium (14020108926, Leica, Mannheim, Germany), frozen in liquid nitrogen and sectioned using a Leica RM2016 microtome. Muscle fibre type (I, IIa, and IIb/IIx) in soleus and EDL muscle was determined using myosin ATPase staining as previously described [32]. After staining, pictures were taken on sections using an Olympus BX53F microscope (Tokyo, Japan).
Immunofluorescence staining and imaging
Immunofluorescence staining of GLUT4 was performed in single muscle fibres as previously described [27]. Images were photographed using a Leica confocal microscope.
Statistical analysis
Data are given as mean ± SEM. Two-group comparisons were carried out using Student’s t test, and multiple-group comparisons were performed with two-way ANOVA using Prism software (GraphPad, San Diego, CA, USA). Differences were considered statistically significant at p < 0.05.
Results
Deletion of Ampkα1/α2 in skeletal muscle blunted the hypoglycaemic effect of AICAR and AICAR-stimulated muscle glucose uptake
It has been shown that overexpression of the dominant inhibitory mutant of AMPK in skeletal and cardiac muscle attenuates AICAR-induced hypoglycaemia [9]. To further determine the contribution of skeletal muscle AMPK to AICAR-induced hypoglycaemia, we generated an Ampkα1/α2-mKO mouse model in which both AMPK α1 and α2 subunits were specifically deleted in skeletal muscle. Deletion of AMPK α1 and α2 subunits caused a substantial decrease in TBC1D1-Ser231 phosphorylation but did not alter expression of TBC1D1 and AS160 in skeletal muscle (Fig. 1a). Expression and phosphorylation of AMPKα and TBC1D1 remained normal in other tissues analysed (Fig. 1b, c). Intraperitoneal injection of AICAR caused hypoglycaemia in the Ampkα1/α2 f/f control mice (Fig. 1d). Interestingly, this hypoglycaemic effect of AICAR was blunted in the Ampkα1/α2-mKO mice (Fig. 1d). As expected, glucose uptake was completely inhibited in the AMPKα1/α2-deficient soleus and EDL muscle in response to AICAR (2 mmol/l) (Fig. 1e, f). Concomitantly, AICAR-stimulated GLUT4 translocation was impaired in EDL muscle from the Ampkα1/α2-mKO mice (Fig. 1g, h). These data confirm previous reports that AMPK controls AICAR-stimulated muscle glucose uptake and GLUT4 translocation [6–10, 33], and also demonstrate that muscle AMPK makes a large, if not complete, contribution to AICAR-induced hypoglycaemia under the conditions used in this study.
The Tbc1d1 Ser231Ala-knockin mutation attenuated the hypoglycaemic effect of AICAR
Next, we employed the Tbc1d1 Ser231Ala-knockin mice to investigate whether TBC1D1 mediates the AICAR-induced hypoglycaemic effect downstream of AMPK. Intraperitoneal injection of AICAR induced phosphorylation of AMPK and a well-established bone fide AMPK substrate acetyl-CoA carboxylase (ACC) in knockin muscle to a similar extent as in wild-type (WT) muscle (Fig. 2a). In contrast and as anticipated, AICAR-stimulated TBC1D1-Ser231 phosphorylation was detected only in muscle extracts from the WT mice but not knockins (Fig. 2a). The AICAR-stimulated 14-3-3−TBC1D1 interaction assessed by a 14-3-3 overlay assay was also pronouncedly reduced (by over 80%) in TBC1D1 immunoprecipitates from the knockin muscle extracts compared with the WT controls (Fig. 2a). As previously reported [27], expression of AS160 remained normal in Tbc1d1 Ser231Ala-knockin muscle (Fig. 2a). Interestingly, the hypoglycaemic effect of AICAR was significantly attenuated in both male and female Tbc1d1 Ser231Ala-knockin mice (6–8 weeks old) (Fig. 2b, c). We previously showed that the Tbc1d1 Ser231Ala-knockin mice were tolerant to glucose administration at a young age (less than 4 months old) [27]. In agreement with this report, insulin injection lowered blood glucose levels to a similar extent in the young Tbc1d1 Ser231Ala-knockin mice and WT littermates (10–12 weeks old) (Fig. 2d).
AICAR-stimulated glucose uptake was decreased in skeletal muscle from the Tbc1d1 Ser231Ala-knockin mice
We first examined whether soleus and EDL muscles exhibit different sensitivity to AICAR in the glucose uptake assay as they have different fibre compositions. To this end, two concentrations of AICAR were used to stimulate glucose uptake in WT soleus and EDL muscles. Glucose-uptake rates stimulated by a low concentration of AICAR (0.25 mmol/l) were ∼55% and ∼94% of those stimulated by the high concentration of AICAR (2 mmol/l) in soleus and EDL muscles, respectively (Fig. 3a), suggesting that EDL muscle is more sensitive to AICAR treatment.
We next investigated whether the Tbc1d1 Ser231Ala-knockin mutation impaired AICAR-stimulated muscle glucose uptake. Glucose uptake was normal in soleus and EDL muscles from the Tbc1d1 Ser231Ala-knockin mice under basal conditions. Upon stimulation with AICAR (2 mmol/l), glucose-uptake rates in the knockin soleus muscle were nearly 20% lower (p = 0.0501) than those in the WT muscle (Fig. 3b). Upon stimulation with the high concentration of AICAR (2 mmol/l), glucose-uptake rates were only marginally lower (not statistically significant) in the knockin EDL muscle than in the WT muscle (Fig. 3c). Interestingly, upon stimulation with a low concentration of AICAR (0.15 mmol/l), glucose uptake rates in the knockin EDL muscle were significantly lower, by ∼17%, than those in the WT muscle (Fig. 3c). These data show that the Tbc1d1 Ser231Ala-knockin mutation moderately impaired AICAR-stimulated muscle glucose uptake.
Fibre composition of both soleus and EDL muscles from the Tbc1d1 Ser231Ala-knockin mice had no obvious differences compared with the corresponding muscles from the WT littermates (Fig. 4a–c), indicating that muscle fibre type switch is not the cause of impaired AICAR-stimulated glucose uptake in skeletal muscle from the Tbc1d1 Ser231Ala-knockin mice.
AICAR-stimulated GLUT4 translocation was impaired in EDL muscle from the Tbc1d1 Ser231Ala-knockin mice
GLUT4 is the major glucose transporter that mediates AICAR-stimulated glucose uptake and it undergoes subcellular translocation to the plasma membrane on AICAR stimulation [9]. As previously reported [27], GLUT4 expression was normal in EDL muscle from the Tbc1d1 Ser231Ala-knockin mice (Fig. 5a, b). However, the GLUT4 content of the cell surface determined using a photolabelling method was increased by only ∼25% in ex vivo EDL muscle from the Tbc1d1 Ser231Ala-knockin mice upon stimulation with 0.15 mmol/l AICAR, in contrast to an increase of ∼53% in WT control muscle (Fig. 5c), suggesting that defects in GLUT4 translocation underlie the impaired glucose transport in skeletal muscle from the Tbc1d1 Ser231Ala-knockin mice. We also confirmed that AICAR increased phosphorylation of AMPK and ACC in ex vivo EDL muscle from both genotypes to similar extents (Fig. 5a). As expected, AICAR stimulated TBC1D1-Ser231 phosphorylation in ex vivo EDL muscle from the WT mice but not from the Tbc1d1 Ser231Ala-knockins (Fig. 5a).
The Tbc1d1 Ser231Ala-knockin mutation did not impair exercise performance or exercise-induced muscle glucose uptake in mice
It has been well established that AMPK regulates exercise performance and exercise-induced muscle glucose uptake [34]. We sought to find whether TBC1D1-Ser231 phosphorylation might mediate these effects of AMPK. The Tbc1d1 Ser231Ala-knockin mice displayed normal exercise performance in both power and endurance running tests (Fig. 6a–d). Phosphorylation of AMPK and ACC was increased in the exercised muscle from both genotypes to similar extents (Fig. 6e). In contrast, phosphorylation of TBC1D1-Ser231 was increased only in the exercised muscle from WT mice, and not that from Tbc1d1 Ser231Ala-knockins (Fig. 6e). Exercise robustly increased muscle glucose uptake, which was comparable in skeletal muscle from the Tbc1d1 Ser231Ala-knockin mice and WT littermates (Fig. 6f, g). The unaltered exercise-induced muscle glucose uptake was consistent with the recently reported normal contraction-stimulated glucose uptake in skeletal muscle from the Tbc1d1 Ser231Ala-knockin mice [27].
Discussion
Here we employed the Tbc1d1 Ser231Ala-knockin mouse to study the in vivo function of TBC1D1-Ser231 phosphorylation and its subsequent 14-3-3 binding in mediating AMPK-governed muscle glucose uptake. A major finding of this study is that TBC1D1-Ser231 phosphorylation and/or its binding to 14-3-3s regulates AICAR-mediated glucose metabolism at both peripheral and whole-body levels downstream of AMPK.
AICAR is widely used as a tool compound to study AMPK signalling, and it lowers blood glucose in an AMPK-dependent manner when administered to mice. Overexpression of a kinase-dead mutant form of AMPKα2 or genetic ablation of AMPK α2 or β2 can attenuate the hypoglycaemic effect of AICAR [6–9]. The contribution of AMPK to AICAR-induced hypoglycaemia in various organs may depend on the fasting status of mice. For instance, our data demonstrate that muscle AMPK makes a large, if not complete, contribution to AICAR-induced hypoglycaemia in mice subjected to partial fasting for 4 h. This hypoglycaemic effect is most likely caused by AICAR-stimulated glucose uptake in skeletal muscle, which is blunted in the Ampkα1/α2-mKO mice. However, in overnight-fasted mice, liver AMPK also partially mediates AICAR-induced hypoglycaemia [35]. Our Tbc1d1 Ser231Ala-knockin mouse is a whole-body knockin model, which has more lean mass and normal fat mass at a young age (less than 4 months old) [27]. Even though skeletal muscle plays a dominant role in AICAR-induced hypoglycaemia in mice subjected to partial fasting (4 h), it is still possible that the Tbc1d1 Ser231Ala-knockin mutation might impair AICAR-induced hypoglycaemia through multiple organs including skeletal muscle. The Tbc1d1 Ser231Ala-knockin mice have higher plasma IGF1 levels that activate the protein kinase B (PKB) pathway in skeletal muscle. Consequently, the PKB activates the downstream mTOR pathway, but does not phosphorylate AS160, which is a key regulator for muscle glucose uptake [27]. Currently, we do not know whether IGF1 levels can affect AICAR-induced hypoglycaemia in mice.
It is worth noting that the Ampkα1/α2-mKO mice were completely resistant to the hypoglycaemic effect of AICAR, and glucose uptake was blunted in isolated skeletal muscle from our Ampkα1/α2-mKO mice (this study) and other AMPK mouse models, even with the high concentration of AICAR (2 mmol/l) [6–10]. In contrast, the Tbc1d1 Ser231Ala-knockin mice were only partially resistant to the hypoglycaemic effect of AICAR, and glucose uptake was impaired in their EDL muscle only in response to the low concentration of AICAR (0.15 mmol/l) but not the high concentration of AICAR (2 mmol/l). Moreover, the Tbc1d1 Ser231Ala-knockin mutation did not impair contraction/exercise-stimulated glucose uptake in skeletal muscle (this study and Chen et al [27]), which is partially mediated by AMPK [9, 34]. One possible explanation is that other potential AMPK sites such as Thr499, Ser621, Ser660 and Ser700 on TBC1D1 [19, 36] might, together with Ser231, account for the regulation of glucose uptake in response to the high concentration of AICAR (2 mmol/l) or muscle contraction. In support of this notion, overexpression of a TBC1D1-4P mutant (in which Ser231, Thr499, Thr590 and Ser621 were mutated to Ala) decreased contraction-stimulated glucose uptake in mouse skeletal muscle [20]. A second possibility is that the related RabGAP AS160 might contribute to AICAR- or contraction-stimulated glucose uptake. AICAR as well as muscle contraction could stimulate Ser595 phosphorylation of AS160, though its role in mediating AICAR- or contraction-stimulated glucose uptake remains to be established [22]. Third is the likelihood that factors other than TBC1D1 and AS160 might be responsible for muscle glucose uptake in response to the high concentration of AICAR (2 mmol/l) and muscle contraction. For example, guanine nucleotide exchange factors (GEFs) antagonise the effects of RabGAPs on activation of downstream Rabs, and can potentially regulate GLUT4 trafficking and glucose uptake. One such GEF, DENN/MADD domain containing 4C (Dennd4C), has recently been identified as an important factor in regulating GLUT4 trafficking in adipocytes [37]. It is conceivable that such GEFs also exist in muscle and regulate GLUT4 translocation in response to AICAR or muscle contraction. As the TBC1D1-Ser231 phosphorylation and/or its binding to 14-3-3s play only a moderate role in mediating AMPK-governed muscle glucose uptake, these knockin mice should be useful in helping to identify other putative mediators of AMPK-regulated muscle glucose uptake in the future.
A growing body of evidence shows that genetic manipulation or mutation of the related RabGAPs, TBC1D1 and AS160, regulates GLUT4 by modulating both its expression and translocation in a complex manner. Insulin-stimulated GLUT4 translocation to the plasma membrane was impaired in the As160 Thr649Ala-knockin mice, although expression of the transporter was elevated [25]. In contrast, expression of GLUT4 was decreased in various muscles and adipose tissue of the AS160-deficient mouse models [38–40] as well as in skeletal muscles from human patients who harbour a premature stop codon Arg684Ter on AS160 [15]. Similarly, here we show that the Tbc1d1 Ser231Ala-knockin mutation did not affect GLUT4 expression but attenuated GLUT4 translocation to the plasma membrane in response to AICAR, whereas a natural TBC1D1-deficient mutant mouse and various Tbc1d1-knockout mice had reduced levels of GLUT4 in their skeletal muscle [14, 41–44]. Interestingly, the reduced GLUT4 levels in one of the Tbc1d1-knockout mouse models caused a decrease in exercise-stimulated glucose uptake in non-oxidative muscle fibres and consequently impaired exercise endurance [44]. It has been recently shown that the loss of AS160 GTPase-activating protein (GAP) activity accelerates lysosomal degradation of GLUT4 [40]. A key question remaining to be answered in all the TBC1D1 mouse models is whether the effects on GLUT4 translocation or expression depend on the GAP activity of TBC1D1.
In summary, the Tbc1d1 Ser231Ala-knockin mutation impacts on insulin-independent/AICAR-mediated whole-body glucose homeostasis in mouse at least in part through impairing muscle GLUT4 translocation and glucose uptake.
Abbreviations
- ACC:
-
Acetyl-CoA carboxylase
- AICAR:
-
5-Aminoimidazole-4-carboxamide-1-β-d-ribofuranoside
- AMPK:
-
AMP-activated protein kinase
- Ampkα1/α2-mKO:
-
Skeletal muscle-specific Ampkα1/α2-knockout
- AS160:
-
Akt substrate of 160 kDa
- EDL:
-
Extensor digitorum longus
- GAP:
-
GTPase-activating protein
- GEF:
-
Guanine nucleotide exchange factor
- PKB:
-
Protein kinase B
- RabGAP:
-
Rab GTPase-activating protein
- TBC1D1:
-
Tre-2/USP6, BUB2, cdc16 domain family member 1
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Acknowledgements
We thank D. G. Hardie (University of Dundee, Dundee, UK) for the AMPKα1 and AMPKα2 antibodies, and G. Holman (University of Bath, Bath, UK) for the GLUT4 antibody.
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We thank the Ministry of Science and Technology of China (grant numbers 2014CB964704 and 2014BAI02B01 [National Key Scientific Research Program of China], grant number 2014AA021104 [National High Technology Research and Development Program of China]), the National Natural Science Foundation of China (grant numbers 31271498 and 31571211), the Ministry of Education of China (grant numbers 20120091120048 and NCET-13-0270) and the UK Medical Research Council (grant number U127084354) for financial support.
Contribution statement
QLC, BXX, SSZ, PR, YS, SD, LC, CQ and ML performed the experiments, analysed the data and reviewed and edited the manuscript. KS and CM participated in the experimental design and reviewed and edited the manuscript. SC and HYW designed and performed the experiments, analysed the data and wrote the manuscript. All authors approved the final version of the manuscript. SC is the guarantor of this work.
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KS is an employee of the Nestlé Institute of Health Sciences SA, Switzerland. All other authors declare that there is no duality of interest associated with their contribution to this manuscript.
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Qiaoli Chen and Bingxian Xie contributed equally to this study.
Shuai Chen and Hong Yu Wang are joint senior authors.
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Chen, Q., Xie, B., Zhu, S. et al. A Tbc1d1 Ser231Ala-knockin mutation partially impairs AICAR- but not exercise-induced muscle glucose uptake in mice. Diabetologia 60, 336–345 (2017). https://doi.org/10.1007/s00125-016-4151-9
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DOI: https://doi.org/10.1007/s00125-016-4151-9