Abstract
The Bs2 resistance gene of pepper confers resistance against the bacterial pathogen Xanthomonas campestris pv. vesicatoria. As a first step toward isolation of the Bs2 gene, molecular markers tightly linked to the gene were identified by randomly amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP) analysis of near-isogenic lines. Markers flanking the locus were identified and a high-resolution linkage map of the region was developed. One AFLP marker, A2, was found to cosegregate with the locus, while two others, F1 and B3, flank the locus and are within 0.6 cM. Physical mapping of the A2 and F1 markers indicates that these markers may be within 150 kb of each other. Together, these results indicate that the Bs2 region may be cloned either by chromosome walker or landing. The linked markers were also used to characterize gamma-irradiation-induced mutants at the Bs2 locus.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Author information
Authors and Affiliations
Additional information
Received: 15 January 1999 / Accepted: 11 May 1999
Rights and permissions
About this article
Cite this article
Tai, T., Dahlbeck, D., Stall, R. et al. High-resolution genetic and physical mapping of the region containing the Bs2 resistance gene of pepper. Theor Appl Genet 99, 1201–1206 (1999). https://doi.org/10.1007/s001220051325
Issue Date:
DOI: https://doi.org/10.1007/s001220051325