Abstract
Although it is possible to transform wheat, broad application of the technology is limited because of the low overall efficiency and the lack of reliability of the technique. In addition there is little published data on transgene integration patterns and inheritance in wheat. We have generated a population of transgenic wheat and tritordeum lines under different auxin regimes and show that, under the conditions described, the presence of picloram results in higher transformation efficiencies than the presence of 2,4-D. Molecular analysis shows low-copy numbers and simple integration patterns to be prevalent in the transgenic lines. Mendelian inheritance of transgenes in T 1 progeny was observed for the majority of lines.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Author information
Authors and Affiliations
Additional information
Received: 2 December 1997 / Accepted: 12 March 1998
Rights and permissions
About this article
Cite this article
Barro, F., Cannell, M., Lazzeri, P. et al. The influence of auxins on transformation of wheat and tritordeum and analysis of transgene integration patterns in transformants. Theor Appl Genet 97, 684–695 (1998). https://doi.org/10.1007/s001220050944
Issue Date:
DOI: https://doi.org/10.1007/s001220050944