Abstract
The F0-F1 ATPase subunit 9 gene of carrot mitochondria has been isolated from both petaloid male-sterile (atp9-1) and normal fertile cytoplasms (atp9-3). The position occupied in atp9-3 by the TAA stop codon is, in the case of atp9-1 replaced by the CAA triplet coding for glutamine, which makes this latter ORF 13 amino acid residues longer than that of the atp9-3. The 3’ end of atp9-3 gene is flanked by a direct repeat of 42 bp. The sequence of the repeat unit is also present at the 3’ end of atp9-1 but without reiteration. A truncated and presumably inactive version of atp9 (atp9-2) was found to be present in cytoplasms regardless of the fertility phenotype which they condition. The atp9-1 gene from petaloid cytoplasm appeared to be co-transcribed with the gene coding for 5S rRNA, and nuclear background influenced the accumulation of the respective transcript. The results are discussed with respect to a potential role of atp9-1 in generating the petaloid form of CMS.
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Received: 29 March 1999 / Accepted: 30 April 1999
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Szklarczyk, M., Oczkowski, M., Augustyniak, H. et al. Organisation and expression of mitochondrial atp9 genes from CMS and fertile carrots. Theor Appl Genet 100, 263–270 (2000). https://doi.org/10.1007/s001220050035
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DOI: https://doi.org/10.1007/s001220050035