Abstract:
Objective and Design: This study was designed to elucidate action mechanisms of four anthraquinones identified from Polygonum hypoleucum Ohwi (P. hypoleucum Ohwi) on primary human T lymphocytes.¶Material and methods: The cells were isolated from peripheral blood.¶Treatment: T cells were treated with 5 to 60 μM of four anthraquinones with or without phytohemagglutinin (PHA; 5 μg/ml) for 3 days. Effects of 4 anthraquinones on T lymphocyte proliferation, production and gene expression of inflammatory cytokines and intracellular free Ca2+ concentration ([Ca2+]i) were determined. Data were assessed with Student's t-test.¶Results: On a percentage basis, emodin had the highest suppressing activity on T lymphocyte proliferation with an IC50 of 11.2 ± 0.6 μM. Emodin decreased cytokine production, IL-2 mRNA expression, and [Ca2+]i in activated T cells.¶Conclusions: We hypothesize that the inhibitory mechanisms of emodin on activated T cells proliferation are related to the impairment of cytokine production, IL-2 mRNA level and [Ca2+]i in the cells.¶
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Received 17 July 2000; returned for revision 28 August 2000; accepted by G.W. Carter 26 September 2000
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Kuo, YC., Meng, HC. & Tsai, WJ. Regulation of cell proliferation, inflammatory cytokine production and calcium mobilization in primary human T lymphocytes by emodin from Polygonum hypoleucum Ohwi . Inflamm. res. 50, 73–82 (2001). https://doi.org/10.1007/s000110050727
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DOI: https://doi.org/10.1007/s000110050727