Abstract.
Objective: Our previous study found that substance P (SP), a sensory neuropeptide, was expressed in the dental pulp of rats during experimental tooth movement. We examined the effects of SP on the production of prostaglandin (PG) E2 and the receptor activator of nuclear factor- B ligand (RANKL) by human dental pulp fi broblast-like (HDPF) cells.
Materials and methods: SP was added to cultured HDPF cells at concentrations ranging from of 10−4 to 10−12 mol/L. PGE2 and soluble RANKL (sRANKL) levels were determined using enzyme-linked immunosorbent assay kits. Gene expression was confi rmed by RT-PCR analysis. Pit formation assays using dentin slices were carried out to examine the effect of SP on osteoclastogenesis.
Results: The levels of PGE2 and sRANKL increased in the presence of SP, though the increases were greater in the experimental groups in both a time- and concentration-dependent manner, and the increase of RANKL was partially mediated by PGE2 . The gene expression of cyclooxygenase (COX)-2 and RANKL was up-regulated, and conditioned medium samples obtained from HDPF cells treated with SP induced bone resorption.
Conclusions: SP stimulated the production of PGE2 and RANKL, and promoted bone resorption. Therefore, SP may be involved in pulpal inflammation and root resorption during orthodontic tooth movement.
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Received 11 April 2005; returned for revision 27 July 2005; returned for final revision 20 September 2005; accepted by M. Katori 2 November 2005
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Kojima, T., Yamaguchi, M. & Kasai, K. Substance P stimulates release of RANKL via COX-2 expression in human dental pulp cells. Inflamm. res. 55, 78–84 (2006). https://doi.org/10.1007/s00011-005-0013-5
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DOI: https://doi.org/10.1007/s00011-005-0013-5