Abstract
Shoot cultures of Alyssum markgrafii O.E. Shulz, endemic nickel hyperaccumulating species of central Balkan, were established and maintained on Murashige and Skoog medium supplemented with 0.2 mg dm−3 benzyladenine (BA). Nickel in form of NiCl2 . 6 H2O was supplemented at 22 different concentrations ranging from 0.0001 to 15 mM but none of them was lethal to cultures. High Ni2+ concentrations (10 mM or more) arrested shoot growth which, upon transfer to Ni-free medium, commenced via axillary bud proliferation. Shoots that developed from axillary buds through the subculture manifested increased tolerance to Ni2+ expressed as shoot elongation. Shoot multiplication and dry biomass production decreased with increase of Ni2+ in medium. Only the accumulation of Ni2+ in tissues increased with Ni2+ content of the medium. Apart from shoot cultures, high Ni2+ accumulation was registered in undifferentiated callus cultured on medium with 0.5 mg dm−3 BA and 0.5 mg dm−3 naphthylacetic acid. Highest content of accumulated Ni was 2.37 μg g−1 (d.m.) in shoots and 2.65 μg g−1 (d.m.) in callus, both measured on medium with 15 mM Ni2+.
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Abbreviations
- BA:
-
benzyladenine
- MS:
-
Murahige and Skoog
- NAA:
-
naphthylacetic acid
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Vinterhalter, B., Vinterhalter, D. Nickel hyperaccumulation in shoot cultures of Alyssum markgrafii . Biol Plant 49, 121–124 (2005). https://doi.org/10.1007/s00000-005-1124-z
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DOI: https://doi.org/10.1007/s00000-005-1124-z