Abstract
Eight compounds exuded from young roots of black locust (Robinia pseudoacacia) were separated by two-dimensional HPTLC, by HPLC and GC, and were identified by spectroscopic methods (ultraviolet/visible spectroscopy and mass spectrometry) as 4′,7-dihydroxyflavone, apigenin, naringenin, chrysoeriol and isoliquiritigenin. Structural assignments were confirmed by comparison with authentic standards. The capacity to induce β-galactosidase activity in Rhizobium sp. NGR234 containing a nod box::lacZ fusion on plasmid pA27 identified these flavonoids and the chalcone as nod gene inducers. This indicates the important role of these compounds in nodulation of this legume tree.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Author information
Authors and Affiliations
Additional information
Received: 26 July 1996 / Accepted: 9 September 1996
Rights and permissions
About this article
Cite this article
Scheidemann, P., Wetzel, A. Identification and characterization of flavonoids in the root exudate of Robinia pseudoacacia . Trees 11, 316–321 (1997). https://doi.org/10.1007/PL00009677
Published:
Issue Date:
DOI: https://doi.org/10.1007/PL00009677