Abstract
Fusarium root rot is one of the most important plant diseases. Chemical fungicides are efficient but they have been much criticized. The present study investigates endophytic actino-bacteria isolated from native plants of the Algerian Sahara in the aim of assessing their ability to exercise biocontrol over this soil-borne fungus, and to highlight their plant growth-promoting traits on seedlings of tomato cv. Mar-mande. A total of 21 endophytic actinobacteria were isolated from plant roots. Six isolates were first selected for the biocontrol trials on the basis of their antifungal properties. In vivo biocontrol activity was measured by the disease incidence on plants grown in infested soils. Four isolates decreased the disease incidence in both sterilized and non-sterilized infested soils, whereas no significant differences were obtained with the control chemical agent. Indole-3-acetic acid and siderophore production, and phosphate solubilization were investigated as plant growth-promoting traits. The isolate ZL2 showed positive results for these mechanisms. Statistical treatment of the data brought out the potential of this isolate as a promising candidate for the biocontrol of root rot in tomato seedlings and in regard to its plant growth-promoting characteristics. Molecular identification was performed by 16S rRNA gene sequence analysis and indicated that Streptomyces sp. ZL2 was related to Streptomyces caeruleatus (99.6% of similarity). Isolate Streptomyces sp. ZL2 enhanced the plant resistance to F. oxysporum f. sp. radicis lycopersici root rot and promoted the growth of tomato seedlings. These properties open up promising perspectives for the possible application of isolate ZL2 in crop protection.
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Abbreviations
- CAS:
-
chrome azurol agar
- DAP:
-
diaminopimelic acid
- DL-DAP:
-
meso isomer of diaminopimelic acid
- FOL:
-
Fusarium oxysporum f. sp. lycopersici
- FORL:
-
Fusarium oxysporum f. sp. radicis lycopersici
- IAA:
-
indole-3-acetic acid
- LL-DAP:
-
levo isomer of diaminopimelic acid
- PCA:
-
principal component analysis
- PVK:
-
Pikovskaya medium
- RF:
-
recti flexibilis
- TCP:
-
tricalcium phosphate
- TLC:
-
thin layer chromatography
- YT:
-
yeast extract-tryptone broth
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Zamoum, M., Goudjal, Y., Sabaou, N. et al. Biocontrol Capacities and Plant Growth-Promoting Traits of Endophytic Actinobacteria Isolated from Native Plants of Algerian Sahara. J Plant Dis Prot 122, 215–223 (2015). https://doi.org/10.1007/BF03356555
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DOI: https://doi.org/10.1007/BF03356555