Abstract
A large number of adventitious buds were induced fromin vitro cultured young inflorescences of haploids of rice. Having been subcultured on solidified subculture media at 26°C for 7 days, the adventitious buds were loaded into 1.8 mL plastic cryotubes with cryoprotectant and kept on ice for 45–60 min. After cooled at a rate of 1.0°C/min down to −40°C, the samples, were kept in liquid nitrogen. The adventitious buds which have been cryopreserved for about 30 days were thawed rapidly in 38–40°C water and then plated on solidified MS medium containing 3% sucrose, 0.5 mg/L NAA and 2.0 mg/L kinetin. After plated, 23%–32% of adventitious buds resumed growth and 15%–22% regenerated plantlets. The results of this work indicated that the adventitious buds derived fromin vitro cultured young inflorescences is a critical factor for the success and subculturing adventitious buds on MS medium containing 3% sucrose and 4% sorbitol or 20% potato extract is essential to the procedure. The effective cryoprotectant is 10% DMSO (dimethyl sulfoximide)+0.5 mol/L sorbitol.
Article PDF
Similar content being viewed by others
Explore related subjects
Discover the latest articles, news and stories from top researchers in related subjects.Avoid common mistakes on your manuscript.
References
Nag K K, Street H E. Carrot embryogenesis from frozen cultured cells.Nature, 1973,245:270–272
Brian W W. Genetic preservationin vitro. In: D. A. Evans, ed.Progress in Plant Cellular and Molecular Biology—Proceedings of the VIIth International Congress on Plant Tissue and Cell Culture. Amsterdam: Elsevier press, 1990. 13–22
Kartha K K.Cryopreservation of Plant Cell and Organs. Boca Raton, Florida: CRC Press Inc, 1985
Tsay S S, Tsay H S, Chao CY. Cytochemical studies of callus development from microspore in cultured anther of rice.Plant Cell Reports, 1986,5: 119–123
Fukai S, Goi M, Tanaka M. Cryopreservation of shoot tips of Caryophyllaceae ornamentals.Euphytica, 1991,56 (2): 149–153
Matsumoto T, Sakai A, Yamada K. Cryopreservation ofin vitro-grown apical meristems of wasabi by vitrification and subsequent high plant regeneration.Plant Cell Reports, 1994,13(8):442–446
Ling D H, Chen W Y, Chen M F,et al. Study on spikelet budding directly from immature panicle culture in rice.Acta Bot Sin, 1985,27(4):435–438
Shu L H, Zhang T B, Zhou M J. The responces of rice young panicles of different ploidies toin vitro culture conditions.Chinese Sci Bulletin, 1985,3:221–224
Author information
Authors and Affiliations
Additional information
Supported by the Natural Science Foundation of Hubei Province
Zhang Zhihong: born in 1963, Lecturer
Rights and permissions
About this article
Cite this article
Zhihong, Z., Zhangli, H. Regenerating plants from cryopreserved adventitious buds of haploids in rice. Wuhan Univ. J. Nat. Sci. 4, 115–117 (1999). https://doi.org/10.1007/BF02827638
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02827638