Summary
Aeration of tissue cultured rare Australian plantsConostylis wonganensis S.D. Hopper (Haemodoraceae);Diplolaena andrewsii Ostenf.;Drummondita ericoides Harvey (Rutaceae);Eremophila resinosa F. Muell. (Myoporaceae);Eucalyptus ‘graniticola’ (Myrtaceae);Lechenaultia pulvinaris C. Gardner (goodeniaceae); andSowerbaea multicaulis E. Pritzel (Liliaceae) has been found to reduce vitrification in sensitive species as well as significantly improving shoot quality and transfer to soil in most study species. A simple 7-mm hole with a double-layer insert of filter paper in the polypropylene screw lids of the culture vessel decreased shoot vitrification over a 4-wk culture period. The method has implications for facilitating the tissue culture of other rare Australian plants and reducing the occurrence of this developmental abnormality.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Bunn, E.; Dixon, K. W.; Langley, M. A. In vitro propagation ofLeucopogon obtectus Benth. (Epacridaceae). Plant Cell Tissue Organ Cult. 19:77–84; 1989.
Bunn, E.; dixon, K. W. In vitro propagation of the rare and endangeredGrevillea scapigera A. S. George (Proteaceae). Hort Science 27(3):261–262; 1992.
Debergh, P.; Vanderschaeghe, A. Mass propagation of in vitro plantlets. Chronica Hortic. 30:1–2; 1990.
Dillen, W.; Buysens, S. A simple technique to overcome vitrification inGypsophila paniculata L. Plant Cell Tissue Organ Cult. 19:181–188; 1989.
Hopper, S.; Van Leeuwen, S.; Brown, A., et al. Western Australia's endangered flora. Department of Conservation and Land Management. W.A. Wildlife Research Centre, P.O. Box 51, Wanneroo, W.A., 6065; 1990.
Jackson, M. B.; Abbott, A. J.; Belcher, A. R., et al. Ventilation in plant tissue cultures and effects of poor aeration on ethylene and carbon dioxide accumulation, oxygen depletion and explant development. Ann. Bot. 67:229–237; 1991.
Kevers, C.; Coumans, M.; Coumans-Giles, M.-F., et al. Physiological and biochemical events leading to vitrification of plants culturedin vitro. Physiol. Plant. 61:69–74; 1984.
Leshem, B. Growth of carnation meristemsin vitro: anatomical structure of abnormal plantlets and the effect of agar concentration in the medium on their formation. Ann. Bot. 52:413–415; 1983.
Leshem, B.; Sachs, T. ‘Vitrified’Dianthus—Teratomatain vitro due to growth factor imbalance. Ann. Bot. 56:613–617; 1985.
Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15:473–479; 1962.
O'Brien, T. P.; McCully, M. E. The study of plant structure: principles and selected methods. Melbourne: Termarcarphi; 1981.
Paques, M.; Boxus, Ph. “Vitrification”: review of literature. Acta Hortic. 212:155–166; 1987.
Williams, R. R.; Taji, A. M. Effect of temperature, gel concentration and cytokinins on vitrification ofOlearia microdisca J. M. Black in vitro shoot cultures. Plant Cell Tissue Organ Cult. 26:1–6; 1991.
Ziv, M. Quality of micropropagated plants—vitrification. In Vitro Cell. Dev. Biol. 27:64–69; 1991a.
Ziv, M. Vitrification: morphological and physiological disorders ofin vitro plants. In: Debergh, P. C.; Zimmerman, R. H., ed. Micropropagation. The Netherlands: Kluwer Academic Publishers; 1991b:45–69.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Rossetto, M., Dixon, K.W. & Bunn, E. Aeration: A simple method to control vitrification and improve in vitro culture of rare australian plants. In Vitro Cell.Dev.Biol.–Plant 28, 192–196 (1992). https://doi.org/10.1007/BF02823316
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02823316