Summary
An enzyme-linked assay for the measurement of amyloglucosidase in commercial enzyme mixtures and crude culture filtrates is described. A method for the synthesis of the substrate employed,p-nitrophenyl β-D-maltoside, is also described. The substrate is used in the presence of saturating levels of β-glucosidase. With a range ofAspergillus sp. culture filtrates, an excellent correlation was found for values obtained with this assay and a conventional assay employing maltose as substrate with measurement of released glucose.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Babers, F.H. and Goebel, W.F. (1934) J.Biol.Chem. 105, 473–480.
Bergmeyer, H.U., Schmidt, F. and Stork, H. (1974) Methods of Enzymatic Analysis (Bergmeyer, H.U. Ed.) Academic Press, New York, 1241–1246.
Brauns, O.H. (1929) J. Am. Chem. Soc. 51, 1820–1831.
Iversen, T and Johansson, R. (1979) Synthesis 823–824.
Kleine, H.P. and Sidhu, R.S. (1988) Carbohydr. Res. 182, 307–312.
Lloyd, J.B. and Whelan, W.J. (1969) J. Anal. Chem. 30, 467–470.
McCleary, B.V., Gibson, T.S., Sheehan, H., Casey, A., Horgan, L. and O'Flaherty, J. (1989) Carbohydr. Res. 185, 147–162.
Pazur, J.H. and Ando, T. (1959) J. Biol. Chem. 234, 1966–1970.
Sabin, R.D. and Wasserman, B.P. (1987) J. Agric. Food. Chem. 35, 649–651.
Somogyi, M. (1952) J. Biol. Chem. 195, 19–23.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
McCleary, B.V., Bouhet, F. & Driguez, H. Measurement of amyloglucosidase usingP-nitrophenyl β-maltoside as substrate. Biotechnol Tech 5, 255–258 (1991). https://doi.org/10.1007/BF02438658
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02438658