Abstract
A laboratory-scale system for mass culture of microalgae in 8-, 20- and 40-L polyethylene bags, was designed. Bags are 16.8 cm diameter and 52 cm (8-L bags), 112 cm (20-L) or 224 cm (40-L) length. The system was tested successfully with two freshwater microalgae,Ankistrodesmus falcatus andScenedesmus incrassatulus, cultured in Bold's Basal medium (prepared with either deionized or tap water). The procedure described is simple, reliable and practical, and enables a very cost-effective production of freshwater microalgae to satisfy any laboratory requirements, and when quantities demanded for special applications can not be met by the standard laboratory culture procedures.
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References
de la Noüe J, de Pauw N (1988) The potential of microalgal biotechnology: a review of production and uses of microalgae. Biotech. Adv. 6: 725–770.
Huntley ME, Nonomura AM, de la Noüe J (1989) Algal culture systems. In Huntley ME (ed.), Biotreatment of Agricultural wastewater. CRC Press, Inc., Boca Raton, Florida: 111–130.
Laing I (1991) Cultivation of marine, unicellular algae. Lab. Leafl. MAFF Direct. Fish. Res., Lowestoft, (67), 31 pp
Laing I, Ayala F (1990) Commercial mass culture techniques for producing microalgae. In: Akatsuka I (ed.), Introduction to Applied Phycology. SPB Academic Publishing, The Hague: 447–477.
Stein JR (1973) Handbook of Phycological Methods. Culture methods and growth measurements. Cambridge University Press, London: 7–24.
Tredici MR, Materassi R (1992) From open ponds to vertical alveolar panels: the Italian experience in the development of reactors for the mass cultivation of phototrophic microorganisms. J. appl. Phycol. 4: 221–231.
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Martínez-Jerónimo, F., Espinosa-Chávez, F. A laboratory-scale system for mass culture of freshwater microalgae in polyethylene bags. J Appl Phycol 6, 423–425 (1994). https://doi.org/10.1007/BF02182159
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DOI: https://doi.org/10.1007/BF02182159