Abstract
Yeast cells ofCandida albicans in lysozyme glucose solution were incubated in a 37° C water bath for 6 hours, spread on the surface of a Sabouraud's agar plate and incubated at 37° C for 18–24 hours. Scattered small colonies were seen on the agar surface compared with the thick full growth of the control culture incubated without lysozyme. Twenty-one strains of 6 standard Candida species of human isolation other thanCandida albicans; C. stellatoidea, C. tropicalis, C. pseudotropicalis, C. krusei, C. parapsillosis, C. guilliermondii, showed essentially the same results asCandida albicans. A constant quantity of lysozyme caused destruction of Candida cells to an equal degree, regardless of varied concentrations of glucose. Dilution of lysozyme greater than 100 times the original (5 mg/ml) showed the same degree of candicidal activity, however, was dependent on the presence of minute amounts of glucose. The presence of NaCl prevented the lysis of Candida by lysozyme in various solutions. Candida cells with lysozyme in glucose solution was incubated for 6 hours in a 37° C water bath. Microscopic observations revealed drastic changes in cell morphology. Most of the cells were swollen, degenerated and some completely destroyed. The gram-positive characteristics of Candida cells changed to gram-negative. The combined activity of lysozyme with complement and antibody may play an important role in the protection against Candidiasis in vivo.
Zusammenfassung
Candida albicans-Zellen sind in Lysozyme-glukose-Lösung bei 37° C in Wasserbad für 6 Stunden bebrütet worden; sie sind dann an der Oberfläche von Sabouraud's Agarplatten ausgestrichen und bei 37° C für 18–24 Std. bebrütet worden. Zerstreute, kleine Kolonien sind an der Agarfläche erschienen, im Vergleich mit dem dicken, vollen Wachstum der Kontrolkultur, die ohne Lysozyme bebrütet worden ist. Einundzwanzig Stämme von sechs Standard-Candida Arten aus menschlichen Quellen außerC. albicans: d.h.C. stellatoidea, C. tropicalis, C. pseudotropicalis, C. krusei, C. parapsillosis, C. guilliermondii, zeigten im wesentlichen dasselbe Ergebnis wieC. albicans. Eine konstante Quantität von Lysozyme bewirkte die Zerstörung der Candida-Zellen zu gleichem Grade ohne Rücksicht auf die wechselnde Konzentration der Glukose. Eine großere Verdünnung von Lysozyme als die hundertfache des Originals (5mg/ml) zeigte denselben Grad der candicidalen Aktivität, jedoch war sie von der Gegenwart einer kleinsten Menge von Glukose abhängig. Die Gegenwart von NaCl hat die Lyse von Candida durch Lysozyme in verschiedenen Lösungen verhindert. Candida-Zellen waren mit Lysozyme in Glukoselösung für 6 Std. in Wasserbad bei 37° C bebrütet. Mikroskopische Beobachtung hat einen großen Wechsel in der Zellmorphologie enthüllt. Die meisten Zellen waren geschwollen, degeneriert, und manche völlig zerstört. Die grampositive Eigenart der Candida-Zellen wechselte in die gram-negative. Die vereinigte Aktivität von Lysozyme mit Komplement und Antikörper mag eine wichtige Schutzrolle gegen Candidiasis in vivo spielen.
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Kamaya, T. Lytic action of lysozyme on candida albicans. Mycopathologia et Mycologia Applicata 42, 197–207 (1970). https://doi.org/10.1007/BF02051947
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DOI: https://doi.org/10.1007/BF02051947