Abstract
Weaning, i.e. the change from live to dry food, is a critical stage in the rearing of turbot (Scophthalmus maximus). In order to obtain a more detailed understanding of the associated functional processes, an electron microscopical study of liver morphological alterations before, during and after the weaning process was conducted. Three days before the start of weaning, slight malnutrition of fish was suggested by histological features such as nuclear glycogen deposition, mitochondrial swelling, together with a low level of glycogen and lipid stores. Lipid material was repeatedly located within cisternae of endoplasmic reticulum. Intra-cisternal lipid deposition (steatosis) in the liver was dramatically increased 3 d after the start of weaning. At the end of the weaning period, steatosis disappeared while cytoplasmic lipid deposition was enhanced. Although the weaning process dramatically increased hepatocellular steatosis, this phenomenon is obviously not caused by weaning — since it was already present during the pre-weaning period. As a speculation, steatosis in the liver of larval turbot may be the result of ontogenetic changes in intermediary metabolism, with the magnitude of this process being controlled by nutritional factors.
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Adron, J. W., Blair, A., Cowey, C. B., Shanks, A. M. (1976). Effects of dietary energy source on growth, feed conversion and body composition of turbot (Scophthalmus maximus L.). Aquaculture, Amsterdam 7: 125–132
Bell, R. M., Ballas, L. M., Coleman, R. A. (1981). Lipid topogenesis. J. Lipid Res. 22: 391–403
Bromley, P. J., Howell, B. R. (1983). Factors influencing the survival and growth of turbot larvae,Scophthalmus maximus L., during the change from live to compound feeds. Aquaculture, Amsterdam, 31: 31–40
Bromley, P. J., Sykes, P. A. (1985). Weaning diets for turbot (Scophthalmus maximus L.), sole (Solea solea L.) and cod (Gadus morhua L.). In: Cowey, C. B., Mackie, A. M., Bell, J-G. (eds.) Nutrition and feeding in fish. Academic Press, London, p. 191–216
Braunbeck, T., Görge, G., Storch, V., Nagel, R. (1990). Hepatic steatosis in zebra fish (Brachydanio rerio) induced by long-term exposure to gamma-hexachlorocyclohexane. Ecotoxicol. Envir. Saf. (in press)
Castell, J. D. Tiews, K. (1980). Report of the EIFAC, IUNS and ICES working group on standardization of methodology in fish nutrition research. EIFAC Technical Paper No. 36. Rome: FAO.
Chao, F. F., Stiers, D. L., Ontko, J. A. (1986). Hepatocellular triglyceride synthesis and transfer to lipid droplets and nascent very low density lipoproteins. J. Lipid Res. 27: 1174–1181
Colemann, R. A., Haynes, E. B., Coats C. D. (1987). Ontogeny of microsomal activities of triacylglycerol synthesis in guinea pig liver. J. Lipid Res. 28: 320–325
Cousin, J. C. B., Baudin-Laurencin, F. (1986). Altérations histologiques observées chez des larves de turbot (Scophthalmus maximus L.) en élévage intensif. Aquaculture, Amsterdam 52: 173–189
Deplano, M., Connes, R., Diaz, J. P., Paris, J. (1989). Intestinal steatosis in the farm-reared sea bassDicentrarchus labrax. Dis. aquat. Org. 6: 121–130
Glaumann, H. J., Bergstrand, A., Ericsson, J. L. (1975). Studies on the synthesis and intracellular transport of lipoprotein particles in rat liver. J. Cell Biol. 64: 356–377
Goodridge, A. G., Garay, A., Silpananta, P. (1974). Regulation of lipogenesis and the total activities of lipogenic enzymes in a primary culture of hepatocytes from prenatal and early postnatal chicks. J. biol. Chem. 24: 1469–1475
Hamilton, R. L., Guo, L. S. S., Felker, T. E., Chao, Y. S., Havel, R. J. (1986). Nascent high density lipoproteins from liver perfusates of orotic acid-fed rats. J. Lipid Res. 27: 967–978
Hay, R., Fleming, R., O'Connell, W., Kirschner, J., Oppliger, W. (1988). Apolipoproteins of the orotic fatty acid liver: implications for the biogenesis of plasma lipoproteins. J. Lipid Res. 29: 981–995
Iglesias, J., Olmedo, M., Otero, J. J., Peleteiro, J. B., Solarzano, M. R. (1987). Growth, under laboratory conditions, of turbot,Scophthalmus maximus, from the Rio de Vigo (north-west Spain). Mar. Biol. 96: 11–17
Inui, Y., Tagawa, M., Miwa, Hirano, T. (1989). Effects of bovine TSH on the tissue thyroxine level and metamorphosis in prometamorphic flounder larvae. Gen. comp. Endocr. 74: 406–410
Jones, A., Prickett, R. A., Douglas, M. T., (1981). Recent developments in techniques for rearing marine flatfish larvae, particularly turbot (Scophthalmus maximus L.) on a pilot commercial scale. Rapp. P.v. Reún. Cons. perm. int. Explor. Mer 178: 522–526
Karnovsky, M. (1971). Use of ferrocyanide-reduced osmium tetroxide in electron microscopy. J. Cell Biol. 51: 284
Lie, O., Lied, E., Lambertsen, G. (1986). Liver retention of fat and fatty acids in cod (Gadus morhua) fed different oils. Aquaculture, Amsterdam 59: 187–196
Lombardi, M. D. (1966). Considerations on the pathogenesis of fatty liver. Lab. Invest. 15: 1–20
Miglavs, I., Jobling, M. (1989). The effects of feeding regime on proximate body composition and patterns of energy deposition in juvenile Arctic charr,Salvelinus alpinus. J. Fish Biol. 35: 1–11
Munilla-Moran, R., Barbour, A., Stark, J. R. (1989). NADPH generation in turbot larvae: a practical approach. World Aquaculture 20: 78–79
Pearse, A. G. E. (1961). Histochemistry. Theoretical and applied, 2nd edn. Churchill, London
Phillips, M. J., Poucell, S., Patterson, J., Valencia, P. (1987). The liver: an atlas and text of ultrastructural pathology. Raven Press, New York
Richardson, K. C., Jarett, L., Finke, E. H. (1960). Embedding in epoxy resins for ultrathin sectioning in electron microscopy. Stain Technol. 35: 313–323
Roberts, R. J., Bullock, A. M. (1989). Nutritional pathology. In: Halver, J. E. (ed.) Fish nutrition. Academic Press, London, New York, p. 423–473
Roque, A. L., Jafarey, N. A., Coulter, P. (1965). A stain for the histochemical demonstration of nucleic acids. Expl. Molec. Pathol. 4: 266–274
Sachs, L. (1984). Angewandte Statistik. Springer, Berlin, Heidelberg, New York
Segner, H., Braunbeck, T. (1988). Hepatocellular adaptation to extreme nutritional conditions in ide,Leuciscus idus melanotus L. (Cyprinidae). A morphofunctional analysis. Fish Physiol. Biochem. 5: 79–97
Segner, H., Braunbeck, T. (1990a). Adaptive changes of liver composition and structure in golden ide during winter acclimatization. J. exp. Zool. (in press)
Segner, H., Braunbeck, T. (1990b). Qualitative and quantitative assessment of the response of milkfish, Chanos chanos, fry to low-level copper exposure. In: Perkins, F. O., Cheng, T. O. (eds.) Pathology in marine science. Academic Press, New York, p. 347–364
Segner, H., Möller, H. (1984). Electron microscopical investigations on starvation-induced liver pathology in floundersPlatichthys flesus. Mar. Ecol. Prog. Ser. 19: 193–196
Segner, H., Schmidt, H., Poeppinghausen, K. J. von., Jäger, T. Quantz, G., Witt, U. (1988). Studien zum Einsatz eines industriellen Weaningfutters für die Aufzucht von Steinbutt (Scophthalmus maximus L.). In: Rosenthal, M., Saint-Paùl, U., Hilge, V. (eds.) Perspektiven der Deutschen Aquakultur. Institut für Fischerei und Hydrobiologie Hamburg, p. 51–62
Spurr, A. R. (1969). A low viscosity embedding medium for electron microscopy. J. Ultrastruct. Res. 26: 31–47
Stein, O., Stein, Y. (1967). Lipid synthesis, intracellular transport, storage and secretion. I. Electron microscopic radioautographic study of liver after injection of tritiated palmitate or glycerol in fasted or ethanol-treated rats. J. Cell Biol. 33: 319–339
Storch, V., Stählin, W., Juraio, J. V. (1983). Effect of different diets on the ultrastructure ofChanos chanos fry (Chanidae: Teleostei): an electron microscopic and morphometric analysis. Mar. Biol. 74: 101–104
Timoshava, L. V. (1981). Seasonal changes in the structure of the liver of the plaice,Pleuronectes platessa. J. Ichthyol. (USSR). 21: 145–151
Vernier, J. M. (1975). Etude ultrastructurales des lipoprotines hepatiques de treś basse densite 'au cours du devel'opement de la truite arc-en-ciel,Salmo gairdneri Rich. J. Microscopie Biol. cell, 23: 39–56
Verreth, J., Storch, V., Segner, H. (1987). A comparative study on the nutritional quality of decapsulatedArtemia cysts, micro-encapsulated egg diets and enriched dry feeds forClarias gariepinus (Burchell) larvae. Aquaculture, Amsterdam 63: 269–282
Weibel, E. R. (1979). Stereological methods, Vol. 1. Academic Press, New York, London
Witt, U., Quantz, G., Kuhlmann, D., Kattner, G. (1984). Survival and growth of turbot larvaeScophthalmus maximus L. reared on different food organisms with special regard to long-chain polyunsaturated fatty acids. Aquaculture Engineering 3: 177–190
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Communicated by O. Kinne, Oldendorf/Luhe
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Segner, H., Witt, U. Weaning experiments with turbot (Scophthalmus maximus): Electron microscopic study of liver. Mar. Biol. 105, 353–361 (1990). https://doi.org/10.1007/BF01316306
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DOI: https://doi.org/10.1007/BF01316306