Abstract
Effects on Ca2+ transport of parathyroid hormone (PTH) and N6,O2′-dibutyryl adenosine 3′,5′-cyclic monophosphate (DB-cAMP) were examined in the rabbit distal nephron segments including the cortical thick ascending limb of Henle's loop (CAL), the connecting tubule (CNT) and the cortical collecting tubule (CCT) by the in vitro perfusion technique. When PTH (10−8 mol·l−1) was added to the bath, efflux of Ca2+ (pmol·mm−1·min−1) was increased from 6.29±1.46 to 7.96±1.66 (P<0.02) in the CAL, and from 8.55±1.30 to 13.73±1.24 (P<0.001) in the CNT, respectively, without changes in influx of Ca2+. The effect of PTH on Ca2+ transport in the CAL, however, was abolished when phosphate concentration in the medium was reduced from 3.0 to 1.0 mmol·l−1. When DB-cAMP (10−3 mol·l−1) was added to the bath, efflux of Ca2+ was also increased from 7.01±0.83 to 9.40±0.82, (P<0.05) in the CAL, and from 13.11±0.89 to 19.74±0.52 (P<0.005) in the CNT, respectively. By contrast, neither PTH nor DB-cAMP affected efflux of Ca2+ in the CCT. PTH did not affect the transepithelial voltage either in the CAL or in the CNT. But in the CNT, DB-cAMP decreased the voltage from −14.1 to −9.4 mV. The response of adenylate cyclase activity to PTH in the collagenase treated isolated nephron segments was also examined. Significant increases in adenylase cyclase activity were observed in the CAL as well as in the CNT with 10−6 mol·l−1 PTH. These data indicate that PTH stimulates Ca2+ transport across the CNT probably via activation of the adenylate cyclase-cyclic AMP system. The hormone may also stimulate Ca2+ transport across the CAL in a special condition where plasma phosphate concentration is elevated.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Agus ZS, Chiu PJS, Goldberg M (1977) Regulation of urinary calcium excretion in the rat. Am J Physiol 232:F545-F549
Agus ZS, Gardner LB, Beck LH, Goldberg M (1973) Effects of parathyroid hormone on renal tubular reabsorption of calcium, sodium, and phosphate. Am J Physiol 224:1143–1148
Buerkert J, Marcus D, Jamison RL (1972) Renal tubular calcium reabsorption after parathyroidectomy. J Clin Invest 51:17a
Bourdeau JE, Burg MB (1980) Effect of PTH on calcium transport across the cortical thick ascending limb of Henle's loop. Am J Physiol 239:F121-F126
Bourdeau JE, Burg MB (1979) Voltage dependence of calcium transport in the thick ascending limb of Henle's loop. Am J Physiol 236:F357-F364
Burnatowska MA, Harris CA, Sutton RAL, Dirks JH (1977) Effects of PTH and cAMP on renal handling of calcium, magnesium, and phosphate in the hamster. Am J Physiol 233:F514-F518
Burg MB, Grantham J, Abramow M, Orloff J (1966) Preparation and study of fragments of single rabbit nephrons. Am J Physiol 210:1293–1298
Chabardès D, Gagnan-Brunette M, Imbert-Teboul M, Gontcharevskaia O, Montegut M, Clique A, Morel F (1980) Adenylate cyclase responsiveness to hormones in various portions of the human nephron. J Clin Invest 65:439–448
Chabardès D, Imbert M, Motégut M, Morel F (1975) PTH sensitive adenyl cyclase activity in different segments of the rabbit nephron. Pflügers Arch 354:229–239
Chabardès D, Imbert-Tenoul M, Gagnan-Brunette M, Morel F (1978) Different hormonal target sites along the mouse and rabbit nephrons. In: Guder WG, Schmidt U (eds) Current problems in clinical biochemistry, Hans Huber, Berlin, Stuttgart, Wien, pp 447–454
Costanzo LS, Windhager EE (1978) Calcium and sodium transport by the distal convoluted tubule of the rat. Am J Physiol 235:F492-F506
Grantham JJ, Burg MB (1966) Effect of vasopressin and cyclic AMP on permeability of isolate collecting tubules. Am J Physiol 221:255–259
Greger R, Lang F, Oberleithner H (1978) Distal site of calcium reabsorption in the rat nephron. Pflügers Arch 374:153–157
Harris CA, Burnatowska MA, Seely JF, Sutton RAL, Quamme GA, Dirks JH (1979) Effects of parathyroid hormone on electrolyte transport in the hamster nephron. Am J Physiol 236:F342-F348
Honma M, Satoh T Takezawa J, Ui M (1977) An ultrasensitive method for the simultaneous determination of cyclic AMP and cyclic GMP in small-volume samples from blood and tissue. Biochem Med 18:257–273
Imai M (1977) Function of the thin ascending limb of Henle of rats and hamsters perfused in vitro. Am J Physiol 232:F201-F209
Imai M (1978) Calcium transport across the thick ascending limb of Henle's loop perfused in vitro. Pflügers Arch 374:255–263
Imai M (1979) The connecting tubule: a functional subdivision of the rabbit distal nephron segments. Kidney Int 15:346–356
Imai M, Kokko J (1974) Sodium chloride, urea and water transport in the thin ascending limb of Henle. Generation of osmotic gradients by passive diffusion of solutes. J Clin Invest 53:393–402
Imbert M, Chabardès D, Motégut M, Clique A, Morel F (1975) Adenylate cyclase activity along the rabbit nephron as measured in single isolated segments. Pflügers Arch 354:213–228
Kuntziger H, Amiel C, Roinel N, Morel F (1974) Effects of parathyroidectomy and cyclic AMP on renal transport of phosphate, calcium, and magnesium. Am J Physiol 227:905–911
Morel F, Chabardès D, Imbert M (1976) Functional segmentation of the distal tubule by microdetermination of hormone dependent adenylate cyclase activity. Kidney Int 9:264–277
Morel F, Chabardès D, Imbert-Teboul M (1978) Heterogeneity of hormonal control in the distal nephron. In Proc 7th Int Congr Nephrol (Montreal), S Karger, Basel, pp 209–216
Rouse D, Ng RCK, Suki WN (1980) Calcium transport in the pars recta and thin descending limb of Henle of the rabbit, perfused in vitro. J Clin Invest 65:37–42
Shareghi GR, Agus ZS (1979) PTH sensitive magnesium transport in the rabbit cortical thick ascending limb of Henle (CTAL). Kidney Int 16:837
Shareghi GR, Stoner LC (1978) Calcium transport across segments of the rabbit distal nephron in vitro. Am J Physiol 235:F367-F375
Suki WN, Rouse D (1979) Heterogeneity of calcium transport in the medullary and coreical segments of the thick ascending limb of Henle. Kidney Int 16:840
Sutton RAL, Wong NLM, Dirks JH (1976) Effect of parathyroid hormone on sodium and calcium transport in the dog nephron. Clin Sci Mol Med 51:345–351
Torikai S, Imai M (1979) A simple method to determine adenylate cyclase activity in isolated single nephron segments by radioimmunoassay for succinyl adenosine 3′,5′-cyclic monophosphate. Tohoku J Exp Med 129:91–99
Widrow SH, Levinsky NG (1962) The effect of parathyroid extract on renal tubular calcium reabsorption in the dog. J Clin Invest 41:2151–2159
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Imai, M. Effects of parathyroid hormone and N6,O2′-dibutyryl cyclic AMP on Ca2+ transport across the rabbit distal nephron segments perfused in vitro. Pflugers Arch. 390, 145–151 (1981). https://doi.org/10.1007/BF00590197
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00590197