Summary
Haploid plantlets from male fertile and male sterile sugarbeet plants could be induced at frequencies up to 2.2% using ovule culture. Ovary culture on media without charcoal resulted in a similar induction frequency. Plant development was inhibited by callus development originating from the mother tissue. When the callus parts were removed and the ovule transferred to a new medium without 2,4 D, callus formation could be inhibited by adding 0.5% charcoal to the medium. Up to 6.1% haploids were induced. Chromosome counts in leaf tips, chloroplast counts and isozyme patterns revealed that all plants were haploid and originated from the haploid cells of the embryo sac. Root tips showed spontaneous polyploidisation.
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Baenziger PS, Schaeffer GW (1982) Dihaploids via anthers cultured in vitro, in genetic engineering: applications to agriculture. In: Owens LD (ed) Beltsville Symp 7. Rowman and Allanheld, Ottawa, pp 269–284
Bosemark NO (1971) Haploids and homozygous diploids, triploids and tetraploids in sugar beet. Hereditas 69:193–204
Bossoutrot D, Hosemans D (1985) Gynogenesis in Beta vulgaris L.: from in vitro culture of unpollinated ovules to the production of doubled haploid plants in soil. Plant Cell Rep 4:300–303
De Jong JH, De Bock TSM (1978) Use of haploids of Beta vulgaris L. for the study of orcein and giemsa stained chromosomes. Euphytica 27:41–47
De Jong JH (1980) Improved techniques for staining chromosomes of sugar beet and related wild species. Meded Inst Ration Suikerprod 5
Deuter M (1970) Some remarks on methods for the establishment of the degree of ploidy in sugar beet. Genet Pol 11:219–225
Hosemans D, Bossoutrot D (1983) Induction of Haploid Plants from in vitro Culture of Unpollinated Beet Ovules (Beta vulgaris L.). Z Pflanzenzücht 91:74–77
Kharetchko-Savitzkaya EI (1931) Methods and techniques of artificial hybridisation of the sugar beet (Russian with German summary). Works Sci Res Inst Sugar Ind 13:613–632
Kruse A (1980) The potential use of heterosis in Beta vulgaris L. 1. The production of monoploids, pure lines and polycross hybrids. K Vet Landboholjsk Arsskr S: 117–137
Fisher HE (1956) Untersuchungen an Zwillingen von Beta vulgaris L. Züchter 26:136–152
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–495
Rogozinska JH, Goska M, Kurdowicz A (1977) Induction of plants from anthers of Beta vulgaris cultured in vitro. Acta Soc Bot Pol 46:471–479
Vallejos CE (1983) Enzyme activity staining. In: Tanksley SD, Orton TJ (eds) Isozymes in plant genetics and breeding, part A. Elsevier Amsterdam, pp 469–561
Van Geyt JPC (1986) The use of an isozyme marker system in sugarbeet genetics and breeding. PhD dissertation, Vrije Universiteit Brussel
Van Geyt JPC, Jacobs M (1985) Suspension culture of sugarbeet (Beta vulgaris L.): induction and habituation of de-differentiated and self regenerating cell lines. Plant Cell Rep 4:66–69
Van Geyt JPC, Smed E (1984) Polymorphism of some marker enzymes of the sugarbeet (Beta vulgaris L.) investigated by polyacrylamide gel electrophoresis and starch gel electrophoresis. Z Pflanzenzücht 92:295–308
Van Geyt JPC, D'Halluin K, Jacobs M (1985) Induction of nuclear and cell divisions in microspores of sugarbeet (Beta vulgaris L.). Z Pflanzenzücht 95:325–335
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Communicated by L. Alföldi
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Van Geyt, J., Speckmann, G.J., D'Halluin, K. et al. In vitro induction of haploid plants from unpollinated ovules and ovaries of the sugarbeet (Beta vulgaris L.). Theoret. Appl. Genetics 73, 920–925 (1987). https://doi.org/10.1007/BF00289399
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DOI: https://doi.org/10.1007/BF00289399