Abstract
Callus and adventitious roots were induced on leaf segments from shoot culture of Cephaelis ipecacuanha A. Richard on Murashige-Skoog medium containing 2,4-dichlorophenoxyacetic acid, indole-3-acetic acid, 1-naphthaleneacetic acid and kinetin. The contents of emetic alkaloids in calli, roots and root suspension cultures were quantified by HPLC. Roots cultured in solid and liquid Murashige-Skoog media yielded emetine and cephaeline. The amount of the two alkaloids in the root suspension culture was very similar to that of roots from ipecac mother plant grown in a greenhouse. In contrast, calli subcultured on Murashige-Skoog media containing combinations of 2,4-dichlorophenoxyacetic acid and kinetin produced only trace amounts of emetic alkaloids.
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Abbreviations
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- IAA:
-
indole-3-acetic acid
- NAA:
-
l-naphthaleneacetic acid
- Kin:
-
kinetin
- MS:
-
Murashige-Skoog
- EM:
-
emetine
- CP:
-
cephaeline
- DW:
-
dry weight.
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Communicated by F. Constabel
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Teshima, D., Ikeda, K., Satake, M. et al. Production of emetic alkaloid by in vitro culture of cephaelis ipecacuanha A. Richard. Plant Cell Reports 7, 278–280 (1988). https://doi.org/10.1007/BF00272542
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DOI: https://doi.org/10.1007/BF00272542