Abstract
Improvements in ab initio microspore culture of maize are presented using a modified isolation technique, reduced temperature during early stages of culture, and an elevated sucrose level in the culture medium. Blending-isolation, using excised anthers, was less stressful on microspores than pressing anthers against a stainless steel sieve and resulted in a 3-fold increase in the yield of embryo-like structures (ELS). Exposure to reduced temperature (15°C) during the first 4 days of culture improved microspore viability and increased by 2-fold the number of ELS produced. Higher levels of sucrose (8.0–9.5%) also resulted in improved response. Maximum yield in the present study was 92 ELS per 100 anther equivalents, exceeding previously reported values of 15 ELS per 100 anther equivalents for ab initio microspore culture of maize. The increase in the total number ELS produced had no observable effect on their quality as evidenced by the frequency of formation of callus capable of regenerating plants.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Chen Y (1986) In: Hu H, Yang H (eds) Haploid of higher plants in vitro, Springer Verlag, Berlin, pp 3–25
Chu CC (1978) Proc. Symp. Plant Tissue Cult. 1978, Science Press, Peking, pp 43–50
Chuong PV, Beversdorf WD (1985) Plant Sci. 39:219–226
Coumans MP, Sohota S, Swanson EB (1989) Plant Cell Rep. 7:618–621.
Datta SK, Wenzel G (1987) Plant Sci. 48:49–54.
Dunwell JM (1985) In: Bright SWJ, Jones MGK (eds) Cereal tissue and cell culture, Martinus Nijhoff/Dr W Junk Pub., Dordrecht pp 1–44
Heslop-Harrison J, Heslop-Harrison (1970) Stain Technology 45:115–120
Kao KN (1981) Z. Pflanzenphysiol. Bd. 103. S. 437–443.
Kasha KJ, Ziauddin A, Cho, UH (1989) Stadler Genetic Symp. XIX
Ku MK, Cheng WC, Juo LC, Kuan YL, An HP, Huang CH (1978) Proc. Symp. Plant Tissue Culture 1978, Science Press, Peking, pp 35–42
Kohler F, Wenzel G (1985) J. Plant Physiol. 121:181–191
Lichter R (1982) Z. Pflanzenphysiol. Bd. 105. S. 427–434
Marsolais AA, Kasha KJ (1985) Can. J. Bot. 63:2209–2212
Neuhaus G, Spangenberg G, Scheid OM, Schweiger HG (1987) Theor. Appl. Genet. 75:30–36
Pace GM, Reed JN, Ho LC, Fahey JW (1987) Theor. Appl. Genet. 73:863–869
Pescitelli SM, Mitchell JC, Jones AM, Pareddy DR, Petolino JF (1989) Plant Cell Rep. 7:673–676
Pescitelli SM, Petolino JF (1988) Plant Cell Rep. 7:441–444
Petolino JF (1989) In: Proceedings of the Forty-Fourth Annual Corn & Sorghum Industry Research Conference, American Seed Trade Assoc., Washington, D.C.
Petolino JF, Jones AM (1986) Crop Science 26:1072–1074
Petolino JF, Jones AM, Thompson SA (1988) Theor. Appl. Genet. 76:157–159
Sunderland N, Roberts M (1977) Nature 270:236–270
Swanson EB, Coumans MP, Wu SC, Barsby TL, Beversdorf WD (1987) Plant Cell Rep. 6:94–97
Wei ZM (1982) Theor. Appl. Genet. 63:71–73
Wei ZM, Kyo M, Harada H (1986) Theor. Appl. Genet. 72:252–255.
Author information
Authors and Affiliations
Additional information
Communicated by G. C. Phillips
Rights and permissions
About this article
Cite this article
Pescitelli, S.M., Johnson, C.D. & Petolino, J.F. Isolated microspore culture of maize: effects of isolation technique, reduced temperature, and sucrose level. Plant Cell Reports 8, 628–631 (1990). https://doi.org/10.1007/BF00270070
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00270070