Abstract
Cultured cells of Thalictrum minus L. (Ranunculaceae), transferred from culture flasks to a bubble column bioreactor, produced little berberine and turned dark brown, even when supplied with sufficient oxygen. This phenomenon was ascribed to the removal of CO2 from the culture medium by bubbling air, and could be reproduced in flask cultures artificially deprived of CO2. The induction of cell browning by exogenously administered ethylene suggested that CO2 probably acts antagonistically against endogenously generated C2H4. The physiological damage caused by forced aeration could be prevented by adding 2 % CO2 to the air in the bioreactor.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Breuling M, Alfermann AW, Reinhard E (1985) Plant Cell Rep. 4: 220–223.
De Jong E, Schappert HVJ (1972) Soil Sci. 113: 328–333.
Fukuda H, Komamine A (1982) Planta 155: 423–430.
Isenberg FMR (1979) Hort. Rev. 1: 337–394.
Ke D, Saltveit ME Jr. (1988) Plant Physiol. 88: 1136–1140.
Kierstan M, Bucke C (1977) Biotech. Bioeng. 19: 387–397.
Kobayashi Y, Fukui H, Tabata M (1988) Plant Cell Rep. 7: 249–253.
Kobayashi Y, Fukui H, Tabata M (1989) Plant Cell Rep. 8: 255–258.
Linsmaier EM, Skoog F (1965) Physiol. Plant. 18: 100–127.
Luh BS, Phithakpol B (1972) J. Food Sci. 37: 264–268.
Mayer AM, Harel E (1979) Phytochem. 18: 193–215.
Nakagawa K, Konagai A, Fukui H, Tabata M (1984) Plant Cell Rep. 3: 254–257.
Norstadt FA, Porter LK (1984) Soil Sci. Soc. Am. J. 48: 783–789.
Smock RM (1979) Hort. Rev. 1: 301–336.
Smart NJ, Fowler MW (1981) Biotechnol. Lett. 3: 171–176.
Street HE (1977) in “Plant Tissue and Cell Culture” (Street HE, ed.), Blackwell Scientific Publications, Oxford, London, Edinburgh, Melbourne pp. 61–102.
Swain T, Hills WE (1959) J. Sci. Food Agric. 10: 63–68.
Vorlop KD, Klein J (1987) In “Methods in Enzymology” (Mosbach K, ed.) Vol. 135, Academic Press, pp. 259–268.
Wagner F, Vogelmann H (1977) In “Plant Tissue Culture and Its Biotechnolgical Application” (Barz W, Reinhard E and Zenk MH, eds.) Springer-Verlag, Berlin, Heidelberg, New York pp. 245–252.
Warner HL, Leopold AG (1969) Plant Physiol. 44: 156–158.
Yang SF (1969) Plant Physiol. 44: 1203–1204.
Author information
Authors and Affiliations
Additional information
Communicated by G. C. Phillips
Rights and permissions
About this article
Cite this article
Kobayashi, Y., Fukui, H. & Tabata, M. Effect of carbon dioxide and ethylene on berberine production and cell browning in Thalictrum minus cell cultures. Plant Cell Reports 9, 496–499 (1991). https://doi.org/10.1007/BF00232104
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00232104