Abstract
Linkage of randomly amplified polymorphic DNA (RAPD) markers with a single dominant gene for resistance to black root rot (Chalara elegans Nag Raj and Kendrick; Syn. Thielaviopsis basicola [Berk. and Broome] Ferraris) of tobacco (Nicotiana tabacum L.), which was transferred from N. debneyi Domin, was investigated in this study. There were 2594 repeatable RAPD fragments generated by 441 primers on DNAs of ‘Delgold’ tobacco, a BC5F8 near isogenic line (NIL) carrying the resistance gene in a ‘Delgold’ background, and ‘PB19’, the donor parent of the resistance gene. Only 7 of these primers produced eight RAPD markers polymorphic between ‘Delgold’ and ‘PB19’, indicating there are few RAPD polymorphisms between them despite relatively dissimilar pedigrees. Five of the eight RAPD markers were not polymorphic between ‘Delgold’ and the NIL. All of these markers proved to be unlinked with the resistance gene in F2 linkage tests. Of the remaining three RAPD markers polymorphic between ‘Delgold’ and the NIL, two were shown to be strongly linked with the resistance gene; one in coupling and the other in repulsion. Application of the two RAPDs in the elimination of linkage drag associated with the N. debneyi resistance gene and marker-assisted selection for the breeding of new tobacco cultivars with the resistance gene is discussed.
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Bentolila S, Guitton C, Bouvet N, Sailland A, Nykaza S, Freyssinet G (1991) Identification of an RFLP marker tightly linked to the Ht1 gene in maize. Theor Appl Genet 82:393–398
Chungwongse J, Bunn TB, Crossman C, Jiang J, Tanksley SD (1994) Chromosomal location and molecular marker tagging of the powdery mildew resistance gene (hv) in tomato. Theor Appl Genet 89:76–79
Clayton EE (1969) The study of resistance to the black root rot disease of tobacco. Tob Sci 13: 30–37
Edwards K, Johnstone C, Thompson C (1991) A simple and rapid method for the preparation of plant genomic DNA for PCR analysis. Nucleic Acids Res 19: 1349
Goodspeed TH (1954) The genus Nicotiana. Chronica Botanica, Waltham, Mass.
Hartl L, Weiss H, Zeller FJ, Jahoor A (1993) Use of RFLP markers for the identification of alleles of the Pm3 locus conferring powdery mildew resistance in wheat (Triticum aestivum L.). Theor Appl Genet 86:959–963
Hinze K, Thompson RD, Ritter E, Salamini F, Schulze-lefert P (1991) Restriction fragment length polymorphism-mediated targeting of the ml-o resistance locus in barley (Hordeum vulgare). Proc Natl Acad Sci USA 88:3691–3695
Hoffbeck LJ, Neas MO, Heggestad HE, Skoog HA (1965) ‘Burley 49’, a new disease-resistance burley tobacco. Tenn Agric Exp Stn Bull 395
Hsi DCH (1965) Blackbull disease of Valencia peanuts. N M State Univ Res Rep 110
Ishii T, Brar DS, Multani DS, Khush GS (1994) Molecular tagging of genes for brown planthopper resistance and earliness introgressed from Oryza austaliensis into cultivated rice, O. sativa. Genome 37:217–221
Koebner RMD, Shepherd KW (1986) Controlled introgression to wheat of genes from rye chromosome arm 1RS by induction of allosyndesis. Theor Appl Genet 73:197–208
Koebner RMD, Shepherd KW (1988) Isolation and agronomic assessment of allosyndetic recombinants derived from wheat-rye translocation 1DL.1RS, carrying reduced amounts of rye chromatin. In: Miller TE, Koebner RMD (eds) Proc 7th Int Wheat Genet Symp. Bath Press, Bath. England, pp 343–348
Legg PD, Litton CC, Collins GB (1981) Effects of the Nicotiana debneyi black root rot resistance factor on agronomic and chemical traits in burley tobacco. Theor Appl Genet 60:365–368
Litton CC (1983) An efficient greenhouse technique for screening small tobacco seedlings for black root rot resistance. Tob Sci 27:1–2
Lockwood JL, Yoder DE, Smith NA (1970) Thielaviopsis basicola root rot of soybeans in Michigan. Plant Dis Rep 54:849–850
Martin GB, Williams JGK, Tanksley SD (1991) Rapid identification of markers linked to a Pseudomonas resistance gene in tomato by using random primers and isogenic lines, Proc Natl Acad Sci 88:2336–2340
Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions using segregation populations. Proc Natl Acad Sci USA 88:9828–9832
Paran I, Michelmore RW (1991) Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuce. Theor Appl Genet 85: 985–993
Penner GA, Chong J, Levesque-lemay M, Molnar SJ, Fedak G (1993) Identification of a RAPD marker linked to the oat stem rust gene Pg3. Theor Appl Genet 85:702–705
Punja ZK, Chittaranjan S, Gaye MM (1992) Development of black root rot caused by Chalara elegans on fresh market carrots. Can J Plant Pathol 14:299–309
Rogowsky PM, Guidet FLY, Langridge P, Shepherd KW, Koebner RMD (1991) Isolation and characterization of wheat-rye recombinants involving chromosome arm 1DS of wheat. Theor Appl Genet 82:537–544
Ronald PC, Albano B, Tabien R, Abenes L, Wu K, McCouch S, Tanksley SD (1992) Genetic and physical analysis of the rice bacterial blight disease resistance locus, Xa21. Mol Gen Genet 236:113–120
Rothrock CS (1992) Influence of soil temperature, water, and texture on Thielavlopsis basicola and black root rot of Cotton. Phytopathology 82:1202–1206
Schachermayr G, Siedler H, Gale MD, Winzeler H, Winzeler M, Keller B (1994) Identification and localization of molecular markers linked to the Lr9 leaf rust resistance gene of wheat. Theor Appl Genet 88:110–115
Schuller C, Backes G, Fischbeck G, Jahoor A (1992) RFEP markers to identify the alleles on the Mla locus conferring powdery mildew resistance in barley. Theor Appl Genet 84:330–338
Shew HD, Shoemaker PB (1993) Effect of host resistance and soil fumigation on Thielaviopsis basicola and development of black root rot on burley tobacco. Plant Dis 77:1035–1039
Wilkinson CA, Rufty RC, Shew HD (1991) Inheritance of partial resistance to black root rot in burley tobacco. Plant Dis 75:889–892
Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18: 6531–6535
Williamson VM, Ho JY, Wu FF, Miller N, Kaloshian I (1994) A PCR-based marker tightly linked to the nematode resistance gene, Mi, in tomato. Theor Appl Genet 87:757–763
Young ND, Tanksley SD (1989) RFEP analysis of the size of chromosomal segments retained around the Tm-2 locus of tomato during backcross breeding. Theor Appl Genet 77:353–359
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Communicated by G. E. Hart
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Bai, D., Reeleder, R. & Brandie, J.E. Identification of two RAPD markers tightly linked with the Nicotiana debneyi gene for resistance to black root rot of tobacco. Theoret. Appl. Genetics 91, 1184–1189 (1995). https://doi.org/10.1007/BF00220927
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DOI: https://doi.org/10.1007/BF00220927