Abstract
We describe a new technique for isolating microvessels from both brain and brain tumors. This method is relatively quick and provides a microvessel preparation free of contamination by other brain tissue. Using this method, structurally intact microvessels from normal rat brain and from a malignant rat astrocytoma were isolated and characterized with light microscopy, scanning electron microrscopy and transmission electron microscopy. In contrast to microvessels derived from normal rat brain, rat astrocytoma microvessels had endothelial cells with multilayered basement membranes, extensive microvilli on the cell surfaces, and a significant increase in the number of pinocytes in the cytoplasm. Furthermore, astrocytoma microvessel endothelial cells had pleomorphic electron dense nuclei with pale perichromatin, whereas the nuclei of endothelial cells of microvessels derived from normal brain tissue were finely granular and homogeneous with characteristically electron dense perichromatin. The morphologic characteristics of the astrocytoma microvessels are similar to the histologic changes seen in astrocytoma tissue in situ, and correlate well with the known altered functions of brain tumor neovasculature.
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Panula P, Joo F, Rechardt L: Evidence for the presence of viable endothelial cells in cultures derived from dissociated rat brain. Experientia 34: 95–97, 1978
Bowman PD, Betz AL, Ar D, Wolinsky JS, Penney JB, Shivers RR, Goldstein GW: Primary culture of capillary endothelium from the rat brain. In vitro 17: 353–362, 1981
Bowman PD, Ennis SR, Rarey KE, Betz AL, Goldstein GW: Brain microvessel endothelial cells in tissue culture: A model for study of blood-brain barrier permeability. Annals of Neurology 14: 396–402, 1983
Brendel V, Meezan E, Carlson EC: Isolated microvessels: A purified, metabolically active preparation from bovine cerebral cortex. Science 185: 953–955, 1974
Goldstein GW, Betz AL: Recent advances in understanding brain capillary function. Ann Neurol 14: 389–395, 1983
Goldstein GW, Wolinsky JS, Csejtey J, Diamond I: Isolation of metabolically activa capillaries from rat brain. J Neurochem 25: 715–717, 1975
Mrsulja BB, Mrsulja BJ, Fujimoto T, Klatzo TF, Spaez M: Isolation of brain capillaries: A simplified technique. Brain Res 110: 361–365, 1976
DeBault LE, Henriquez E, Hart MN, Cancilla PA: Cerebral microvessels and derived cells in tissue culture. In vitro 17: 480–494, 1981
DeBault LE, Kahn LE, Frommes SP, Cancilla PA: Cerebral microvessels and derived cells in tissue culture: Isolation and preliminary characterization. In vitro 15: 473–487, 1979
Gordon EL, Danielson EP, Winn HR: A technique to establish separate primary cultures of microvascular endothelial cells and astroglial cells from rat fore-brain. The Physiologist 31: A191, 1988
Spence AM, Priestley G: A survey of ethylnitrosourea-induced rat gliomas for the presence of tumor rejection antigens expressed in vivo. Neuropath Appl Neurobiol 7: 63–75, 1981
Ali-Osman F, Spence AM, Berger MS, Caughlan J: Primary cultures of human glial tumor cells and their relationship to histopathology: Neuropath Exp Path 46: 371, 1987
Keebler CM, Reagan JW: Tutorials of Cytology: Compendium on Cytotherapy Techniques. Fourth Ed. 4(2): 16–33, 110, 1976
Spaet TH, Erickson RB: The vascular wall in the pathogenisis of thrombosis. Thromb Diath Haem 21(suppl): 67–72, 1966
Nishio S, Ohta M, Abe M, Kitamura K: Microvascular abnormalities in ethylnitrosurea-induced rat brain tumors: structural basis for altered blood-brain barrier function. Acta Neuropath 59: 1–10, 1983
Deane BR, Lantos PL: The vasculature of experimental brain tumors (Parts 1 and 2). J Neurological Science 49: 55–76, 1981
DeBault LE, Cancilla PA: Some properties of isolated endothelial cells in culture. Adv Exper Med Biol 131: 69–78, 1980
Drewes LD, Lidinsky WA: Studies of cerebral capillary endothelial membrane. Adv Cell Biol 131: 17–27, 1980
Gordon PB, Sussman II, Hatcher VB: Long-term culture of human endothelial cells. In vitro 19(9): 661–671, 1983
Waggener JD, Beggs JL: Vasculature of neural neoplasms. In: Thompson RA and Green JR (eds) Advances in Neurology, Vol. 15. Raven Press, New York, 1976, pp 27–49
Long DM: Capillary ultrastructure and the blood-brain barrier in human malignant brain tumors. J Neurosurg 32: 127–144, 1970
Weller RO, Foy M, Cox S: The development and ultrastructure of the microvasculature in malignant gliomas. Neuropath Appl Neurobiol 3: 307–322, 1977
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Silbergeld, D.L., Ali-Osman, F. Isolation and characterization of microvessels from normal brain and brain tumors. J Neuro-Oncol 11, 49–55 (1991). https://doi.org/10.1007/BF00166997
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DOI: https://doi.org/10.1007/BF00166997