Abstract
We have isolated a cDNA that encodes a 142-kDa protein by immunoscreening of a Schizosaccharomyces pombe expression library with a new antibody, mAb8, that reveals spindle poles and equatorial ring-like structures in several organisms. This cDNA encodes a putative protein which we termed Alm (for abnormal long morphology). The protein is predicted to be a coiled-coil protein, containing a central α-helical domain flanked by non-helical terminal domains. Immunofluorescence analysis showed that Alm1 is localized in the medial region of the cell from anaphase to the end of cytokinesis. Cells carrying an alm1::ura4 + disruption are viable and exhibit an elongated morphology. Homozygous alm1::ura4 + diploids sporulated normally but the spores did not germinate. Spores that have inherited the disruption allele from a heterozygous alm1 + / alm1::ura4 + diploid germinated but generated smaller colonies. We propose that Alm1 participates in the structural organization of the medial region in S. pombe.
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Received: 9 April 1999 / Accepted: 22 October 1999
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Jiménez, M., Petit, T., Gancedo, C. et al. The alm1 + gene from Schizosaccharomyces pombe encodes a coiled-coil protein that associates with the medial region during mitosis. Mol Gen Genet 262, 921–930 (2000). https://doi.org/10.1007/PL00008660
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DOI: https://doi.org/10.1007/PL00008660