Abstract
The present study aimed to evaluate the effects of Zataria multiflora essential oil at different concentrations (0, 100, 200, 300 and 500 ppm) on the growth rate and morphogenesis of Alternaria alternata in in vitro and in vivo conditions. The antifungal activity of the oil was tested by the poisoned food technique (PF) and volatile activity assay (VA). The mycelial growth of the test fungus was completely inhibited at oil concentrations of 500 and 200 ppm in PF and VA assessments, respectively. Determination of conidial germination in the presence of the oil was also carried out and it was found that the oil caused 100% inhibition of conidial germination at the level of 300 ppm. Light Microscopy (LM) showed morphogenic changes in the test fungus with 300 ppm of the oil. These changes included a significant decrease and loss of conidiation, severely collapsed and squashed hyphae, a decreased hyphal diameter and granulation of cytoplasm. The treatment with the oil also resulted in highly lobate and bulbous anomalous structures at the hyphal tips. GC-MS evaluations of the essential oil revealed that the latter consisted of 14 compounds of which carvacrol was the major component. In vivo Z. multiflora oil at 500 ppm effectively reduced the percentage of decayed tomatoes. Findings of the current study suggest that essential oils could be used as an alternative for chemicals for control of postharvest phytopathogenic fungi on fruits or vegetables.
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References
Abbas HK, Tanaka T & Duke SO, 1995. Pathogenicity of Alter- naria alternata and Fusarium moniliforme and phytotoxicity of AAL-toxin and fumonisin B1 on tomato cultivars. J Phytopathol 143, 329–334.
Abdollahi M, Hamzehzarghani H & Saharkhiz MJ, 2011. Effects of the essential oil of Zataria multiflora Boiss, a thyme-like medicinal plant from Iran on the growth and sporulation of Aspergillus niger both in vitro and on lime fruits. J Food Safety 31, 424–432.
Ali MS, Saleem M, Ali Z & Ahmad VU, 2000. Chemistry of Zataria multiflora (Lamiaceae). Phytochemistry 55, 933–936.
Amein T, Wright SAI, Wikström M, Koch E, Schmitt A, Stephan D, Jahn M, Tinivella F, Gullino ML, Forsberg G, Werner S, Wolf van der J & Groot SPC, 2011. Evaluation of non-chemical seed treatment methods for control of Alternaria brassicicola on cabbage seeds. J Plant Dis Protect 118, 214–221.
Basti AA, Misaghi A & Khaschabi D, 2007. Growth response and modelling of the effects of Zataria multiflora Boiss. essential oil, pH and temperature on Salmonella typhimurium and Staphylococcus aureus. LWT-Food Sci Technol 40, 973–981.
Daferera DJ, Ziogas BN & Polissiou MG, 2003. The effectiveness of plant essential oils on the growth of Botrytis cinerea, Fusarium sp. and Clavibacter michiganensis subsp. michi- ganensis. Crop Prot 22, 39–44.
Dayan FE, Cantrell CL & Duke SO, 2009. Natural products in crop protection. Bioorganic and Medicinal Chemistry 17, 4022–4034.
Gandomi H, Misaghi A, Akhondzadeh A, Bokaei S, Khosravi A, Abbasifar A & Jebelli A, 2009. Effect of Zataria multiflora Boiss. essential oil on growth and aflatoxin formation by Aspergillus flavus in culture media and cheese. Food Chem Toxicol 47, 2397–2400.
Hosseinzadeh H, Ramezani M & Salmani G, 2000. Antinoci-ceptive, anti-inflammatory and acute toxicity effects of Zataria multiflora Boiss extracts in mice and rats. J Ethno-pharmacol 73, 379–385
Javidnia K, Tabatabai M & Shaffiee A, 1999. Volatile constitution and antimicrobial activity of Zataria multiflora, population Iran. Iran J Chem pp, 1–5.
Mahanta JJ, Chutia M, Bordoloi M, Pathak MG, Adhikary RK & Sarma TC, 2007. Cymbopogon citratus L. essential oil as a potential antifungal agent against key weed moulds of Pleurotus spp. spawns. Flavour Fragr J 22, 525–530.
Mahmoudi E, 2010. Evaluation of citrus cultivars suscepti-bility to leaf spot disease caused by Alternaria alternata under in vitro condition. J Res Agri Sci 6, 75–81.
Meepagala KM, Sturtz G & Wedge DE, 2002. Antifungal constituents of the essential oil fraction of Artemisia drancunculus L. var. dracunculus. J Agric Food Chem 50, 6989–6992.
Mohagheghzadeh A, Shams-Ardakani M, Ghannadi A & Minaeian M, 2004. Rosmarinic acid from Zataria multiflora tops and in vitro culture. Fitoterapia pp, 315–321.
Naeini A, Ziglari T, Shokri H & Khosravi AR, 2010. Assessment of growth-inhibiting effect of some plant essential oils on different Fusarium isolates. J Mycol Med 20, 174–178.
Pandey DK, Tripathi NN, Tripathi RD & Dixit SN, 1982. Fungitoxic and phytotoxic properties of the essential oil of Hyptis suaveolens (L.) Poit. Z Pflanzenkrankh Pflanzenschutz 89, 344–349.
Ramezani M, Hosseinzadeh H & Samizadeh S, 2004. Anti-nociceptive effects of Zataria multiflora Boiss fractions in mice. J Ethnopharmacol 91, 167–70.
Saleem M, Nazli R, Afza N, Sami A & Ali A, 2004. Biological significance of essential oil of Zataria multiflora Boiss. Natl Prod Res 41, 493–497
Shaffiee A & Javidnia K, 1997. Composition of essential oil of Zataria multiflora. Planta Med 63, 371–372.
Singh J & Tripathi NN, 1999. Inhibition of storage fungi of black gram (Vigna mungo L.) by some essential oils. Flavour Frag J 14, 42–44.
Solaimani B, Ramezani S, Rahimi M & Saharkhiz MJ, 2009. Biological control of postharvest disease caused by Penicil- lium digitutum and P. italicum on stored citrus fruits by Shiraz thyme essential oil. Advanc Environ Biol 3, 249–254.
Soliman KM & Badeaa RI, 2002. Effect of oil extracted from some medicinal plants on different mycotoxigenic fungi. Food Chem Technol 40, 1669–1675.
Sreenivasa MY, Dass RS, Raj APC, Prasad MNN, Achar PN & Janardhana GR, 2011. Assessment of the growth inhibiting effect of some plant essential oils on different Fusarium species isolated from sorghum and maize grains. J Plant Dis Protect 118, 208–213.
Surender P, Janalah C, Reddy VK & Reddy SM, 1987. Anti-fungal activity of secretions of scent glands from Heterop-teram bugs. Indian J Exp Biol 25, 233–234.
Tataoui-Elaraki A, Ferhout H & Errifi A, 1993. Inhibition of fungal asexual reproduction stages by three Moroccan essential oils. J Essen Oil Res 5, 535–545.
Thompson DP, 1989. Fungitoxic activity of essential oil components on food storage fungi. Mycologia 81, 151–153.
Tripathi A, Sharma N & Sharma V, 2009. In vitro efficacy of Hyptis suaveolens L. (Poit.) essential oil on growth and morphogenesis of Fusarium oxysporum f.sp. gladioli (Massey) Snyder & Hansen. World J Microbiol Biotechnol 25, 503–512.
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Mahmoudi, E., Ahmadi, A. & Naderi, D. Effect of Zataria multiflora essential oil on Alternaria alternata in vitro and in an assay on tomato fruits. J Plant Dis Prot 119, 53–58 (2012). https://doi.org/10.1007/BF03356420
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DOI: https://doi.org/10.1007/BF03356420