Abstract
This in vivo study investigated the ability of fluorescent Pseudomonas spp. to suppress Polymyxa betae, a vector of Beet necrotic yellow vein virus causing rhizomania in sugar beet fields. For this purpose, the Pseudomonas putida biotype A (PpA) and biotype B (PpB) were isolated from sugar beet growing areas in Samsun, Turkey and with indicated suppression of P. betae were used against P. betae as a biocontrol agent. Firstly, PpA and PpB were applied to the roots of sugar beet seedlings of rhizomania-susceptible (cv. Arosa) and partially resistant (cv. Leila) cultivars. Statistical differences were not significant among PpA, PpB, Leila and Arosa culti-vars within rhizomania infested soil treatments, and there were also no significant differences among PpB and control groups (P> 0.05). In contrast, the partially resistant cultivar Leila with rhizomania free soil, PpB and negative control were significantly different from the other treatments (P> 0.05). Furthermore, numbers and diameters of the resting spores were significantly reduced by PpA and PpB treatments. In the treatments with PpA and PpB, a positive effect was observed on sugar beet weight and growth.
Zusammenfassung
Die antagonistische Wirkung natürlicher Biotypen von Pseudomonas putida gegenüber Polymyxa betae Keskin, dem Vektor des Rizomaniavirus der Rüben, wurde in vivo untersucht. Zu diesem Zweck wurden die Biotypen A und B von Pseudomonas putida im türkischen Zuckerrübenanbau-gebiet von Samsun isoliert und ihr antagonistisches Potenzial gegenüber P. betae untersucht. Wurzeln Rizomania-anfälliger (cv. Arosa) und teilresistenter (cv. Leila) Zuckerrübensäm-linge wurden mit den Biotypen A und B von P. putida inokuliert. Die Unterschiede zwischen den Biotypen A, B und den beiden Rübensorten waren in den Rizomania-Varianten statistisch genauso wenig signifikant (P< 0.05) wie die Unterschiede zwischen dem Biotyp B und der Kontrolle. Im Gegensatz dazu unterschieden sich die teilresistente Sorte Leila, der Biotyp B von P. putida und die negative Kontrolle in den Rizomania-freien Varianten signifikant (P> 0.05) von allen anderen Behandlungen. Die Anzahl und Größe der Dauersporen von P. betae waren darüber hinaus nach einer Behandlung mit den Biotypen A oder B vermindert, während die Wachstumsraten und das Gewicht der Zuckerrüben in den mit den Antagonisten behandelten Varianten erhöht waren.
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Aksoy, H.M., Yilmaz, N.D.K. Antagonistic effects of natural Pseudomonas putida biotypes on Polymyxa betae Keskin, the vector of Beet necrotic yellow vein virus in sugar beet. J Plant Dis Prot 115, 241–246 (2008). https://doi.org/10.1007/BF03356271
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DOI: https://doi.org/10.1007/BF03356271