Abstract
Molecular analysis of 29 lentil (Lens culinaris) cultivars and landraces of Indian origin was carried out using twenty RAPD and ten cross-species STMS primers. A total of 97 markers (72 RAPD and 25 STMS) were amplified of which 42.3% were polymorphic. Genetic similarity among the cultivars and landraces was 89.7%. The observed results indicated low level of genetic diversity in the studied material. UPGMA cluster analysis for the combined data of RAPD and STMS revealed two broad clusters — Cluster I with three landraces and Cluster II containing all remaining landraces and cultivars except Precoz. Germplasm line Precoz was found to be the most distinct in individual as well as combined analyses. All cultivars and landraces except K-75 and L4076 could be discriminated from one another using combined data for the two techniques. Germplasm lines Precoz, L830 and cultivars L4147 and JL3 were quite distinct and could be potential germplasm resource.
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References
Eser D, Lens, 3 (1976) 15.
Tickoo JL, Sharma B, Mishra SK & Dikshit HK, Indian J Agril Sci, 75 (2005) 539.
Sardana S, Gautam NK, Kumar D, Sapra RL & Mithal SK, Indian J Pl Genet Resour, 11 (1998) 25.
Saghai-Maroof MA, Soliman KM, Jorgensen RA & Allard RW, Proc Natl Acad Sci, USA, 81 (1984) 8014.
Rana MK, Kumari R, Singh S & Bhat KV, J Genet Breed, 58 (2004) 305.
Jaccard P, Bull Soc Vaud Sci Na, 44 (1908) 223.
Rohlf FJ, NTSYS-pc Numerical taxonomy and multivariate analysis system, version 2.1. Exeter Softwae, Setauket, New York (2000).
Excoffier L, Smouse P & Quattro J, Genetics, 131 (1992) 479.
Schneider S, Roessli D, & Excoffier L, Arlequin ver. 2.000: A software for population genetics data analysis. Genetics and Biometry Laboratory, University of Geneva, Switzerland. (http://anthro.unige.ch/arlequin), (2000).
Dixit GP & Katiyaar PK, Indian J Agril Sci, 74 (2004) 625.
Sharma SK, Dawson IK & Waugh R, Theor Appl Genet, 91 (1995) 647.
Abo-elwafa A, Murai K & Shimada T, Theor Appl Genet, 90 (1995) 335.
Ford R, Pang ECK & Taylor PWJ, Euphytica, 96 (1997) 247.
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Rana, M.K., Kumari, R., Singh, S. et al. Genetic Analysis of Indian Lentil (Lens culinaris Medikus) Cultivars and Landraces Using RAPD and STMS Markers. J. Plant Biochem. Biotechnol. 16, 53–57 (2007). https://doi.org/10.1007/BF03321929
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DOI: https://doi.org/10.1007/BF03321929