Abstract
An efficient in vitro plant regeneration from leaf-disc culture of Jatropha curcas L has been established. Adventitious shoot buds along with callus were induced from leaves of 2-year-old J. curcas plants cultured on Murashige and Skoog’s (MS) medium supplemented with TDZ (2 μM) BAP (2 μM) and IBA (1 μM), wherein 63.3% leaf explants responded. The multiplication of shoots was achieved from the adventitious shoot buds after transferring them to shoot induction medium. The highest number of shoots (9.7/explant) was achieved after 6 weeks of culture on MS medium containing 3 μM of BAR The welldeveloped shoots were rooted on MS medium supplemented with IBA (1.5 μM) with the rooting frequency of 53.3%. Addition of phloroglucinol (200 μM) to the medium enhanced the frequency of rooting to 76.7%. Regenerated plantlets were successfully transferred to field after initial acclimatization.
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Abbreviations
- BAP:
-
6-benzylaminopurine
- IBA:
-
indole-3-butydcacid
- MS:
-
Murashige and Skoog
- SE:
-
Standard error
- TDZ:
-
thidiazuron
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Kumar, S., Kumaria, S. & Tandon, P. Efficient In vitro Plant Regeneration Protocol from Leaf Explant of Jatropha curcas L — A Promising Biofuel Plant. J. Plant Biochem. Biotechnol. 19, 273–275 (2010). https://doi.org/10.1007/BF03263356
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DOI: https://doi.org/10.1007/BF03263356