Abstract
EST-SSR markers were developed using sequence information from 1740 expressed sequence tags (ESTs) of finger millet available in the public domain. A set of 31 SSR markers were synthesized based on di, tri, tetra and penta-nucleotide repeat sequences. These were used for PCR analysis of 11 elite germplasm lines of finger millet of Indian and African origin. Out of 31 SSR markers, amplification products were obtained for 17 primer pairs. Of these nine were found polymorphic with two alleles per locus. These 17 SSR primer pairs were also tested for amplification in three varieties of pearl millet (Pennisetum glaucum) and 11 could be transferred to pearl millet. The informative EST SSR markers developed, can be used in finger millet as well as pearl millet genetic improvement projects.
Article PDF
Avoid common mistakes on your manuscript.
Abbreviations
- ESTs:
-
Expressed sequence tags
- SSRs:
-
Simple sequence repeats
References
Hilu KW & Johnson JL, Theor Appl Genet, 83 (1992) 895.
Werth CR, Hilu KW & Langer CA, Am J Bot, 81 (1994) 1186.
Hilu KW, Genome, 38 (1995) 232.
Fakrudin B, Shashidhar HE, Kulkarni RS & Hittalmani S, PGR Newslett, 138 (2004) 50.
Babu BK, Senthil N, Gomez SM, Biji KR, Rajendraprasad NS, Kumar SS & Babu RC, Genet Resour Crop Evol, 54 (2007) 399.
Dida MM, Srinivasachary, Ramakrishnan S, Bennetzen JL, Gale MD & Devos KM, Theor Appl Genet, 114 (2007) 321.
Robinson AJ, Love CG, Batley J, Barker G & Edwards D, Bioinformatics, 20 (2004) 1475.
Saghai-Maroof MA, Soliman KM, Jorgensen RA & Allard RW, Proc Natl Acad Sci, USA, 81 (1984) 8014.
Senthilvel S, Mahalakshmi V, Kumar PS, Reddy AR, Markandeya G, Reddy MK, Misra R & Hash CT, Proc 4 th Intn Crop Science Congress, 26 Sept to 1 Oct 2004, Brisbane, Australia.
Parida SK, Kumar KAR, Dalal V, Singh NK & Mohapatra T, Theor Appl Genet, 112 (2006) 808.
Jia XP, Shi YS, Song YC, Wang GY, Wang TY & Li Y, Genet Resour Crop Evol, 54 (2007) 233.
Kantety RV, Rotal ML, Matthews DE & Sorrells ME, Plant Mol Biol, 48 (2002) 501.
Thiel T, Michalek W & Varshney R et al, Theor Appl Genet, 106 (2003) 411.
Varshney RK, Thiel T, Stein N, Langridge P & Graner A, Cell Mol Biol Lett, 7 (2002) 537.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Arya, L., Verma, M., Gupta, V.K. et al. Development of EST SSRs in Finger Millet (Eleusine coracana ssp coracana) and their Transferability to Pearl Millet (Pennisetum glaucum). J. Plant Biochem. Biotechnol. 18, 97–100 (2009). https://doi.org/10.1007/BF03263303
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF03263303