Abstract
A method of microcloning via somatic embryogenesis was established in date palm (Phoenix dactylifera L) cultivars. The method has potential for commercial application. Embryogenic cultures were initiated from soft primordial tissues of 2-3-year-old female suckers. The system was optimized first for the genotype ’sayar’ and then its applicability was tested to other genotypes. A method of pre-acclimation using pre-acclimation chambers has been devised, which improved hardening survival greatly. More than 80 clones obtained from the genotype ’sayar’ have been shifted to field for agronomic evaluation.
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Abbreviations
- 2,4-0:
-
2,4-dichlorophenoxyacetic acid
- BAP:
-
6-benzyl aminopurine
- IAA:
-
indole-3-acetic acid
- NAA:
-
a-naphthalene acetic acid
- Kn:
-
Kinetin
- MS:
-
Murashige
- Skoog:
-
ECI, Embryogenic callus induction
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Bhargava, S.C., Saxena, S.N. & Sharma, R. In Vitro Multiplication of Phoenix dactylifera (L). J. Plant Biochem. Biotechnol. 12, 43–47 (2003). https://doi.org/10.1007/BF03263158
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DOI: https://doi.org/10.1007/BF03263158