Abstract
The study aims at determining genetic diversity in a set of land races in comparison to a representative sample of improved rice Varieties, using random amplified polymorphic DNA (RAPD). Analysis of 36 accessions using 10 arbitrary decamer random primers, revealed 97.1 6% polymorphism. Similarity values among the land races ranged from 0.58 to 0.89 indicating wide diversity.The extent of diversity of the accessions was assessed using heterozygosity arithmetic mean value in land races (0.71) and cultivars (0.54). Marker Index (MI) revealed higher values in land races (120.83) than improved varieties (51.74).The land races and improved varieties formed separate clusters at 0.65 similarity suggesting that genetically distant land races could be potentially valuable sources for enlarging and enriching the genepool of improved varieties.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Tanksley SD & McCouch SR, Science, 277 (1997) 1063.
Stobdan T, Khanna VK, Singh NK & Mani SC, Rice Biotech Quart, 40 (1999) 18.
Raghunathachari P, Khanna VK, Singh US & Singh NK, Curr Sci, 79 (2000) 994.
Wang ZY & Tanksley SD, Genome, 32 (1989) 1113.
Wu KS & Tanksley SD, Mol Gen Genet, 241 (1993) 225.
Parsons BJ, Newbury HJ, Jackson MT & Ford-Lloyd BV, Mol Breed, 3 (1997) 115.
Singh KN, Nandi R, Shanmugasundaram P, Sadasivam S, Huang N, Brar DS & Khush GS, Genet Res and Crop Evol, 46 (1999) 427.
Williams JGK, Kubelik AR, Livak KJ, Rafalski JA & Tingey SV, Nucleic Acids Res, 18 (1990) 6531.
Welsh J & McClelland M, Nucleic Acids Res, 18 (1990) 7213.
Mackill DJ, Crop Sci, 35 (1995) 889.
Virk PS, Newbury HJ, Jackson MT & Ford-Lloyd BV, Theor Appl Genet, 90 (1995) 1049.
Cho YC, Shin YS, Ahn SN, Gregorio GB, Kang KH, Brar DS & Moon HP, Korean J Crop Sci, 44 (1999) 26.
Ghareyazie B, Huang N, Second G & Bennett J, Theor Appl Genet, 91 (1995) 218.
Cao D & Oard JH, Crop Sci, 37 (1997) 1630.
Davierwala AP, Chowdhari KV, Shiv Kumar, Reddy APK, Ranjekar PK & Gupta VS, Genetica, 108 (2000) 269.
Jena KK & Kochert G, Plant Mol Biol, 16 (1991) 831.
Rohlf FJ, NTSYS. Numerical taxonomyandmultivariatea nalysis system. Version 2.0, (1993).
Nei M, Proc Natl Acad Sci, USA, 70 (1973) 3321.
Powell W, Morgante M, Andre C, Hanafey M, Vogel J, Tingey S. & Rafalski A, Mol Breed, 2 (1996) 225.
Yang GP, Saghai Maroof MA, Xu CG, Zhang O & Biyashev RM, Mol Gen Genet, 245 (1994) 187.
Fuentes JL, Escovar F, Alvarez A, Gallego G, Duque MC, Ferrer M, Deus JE & Tohme JM, Euphytica, 109 (1999) 107.
Virk PS, Zhu J, Newbury HJ, Bryan GJ, Jackson MT & Ford-Lloyd BV, Euphyfica, 112 (2000) 275.
Fanniza G, Colonna G, Resta P & Ferrara G, Euphytica, 107 (1999) 45.
Oka HI, In Origin of cultivated rice, Japan Scientific Societies Press, Tokyo, Elsevier Science Publishers, Amsterdam (1988) p 114.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Neeraja, C.N., Sarla, N. & Siddiq, E.A. RAPD Analysis of Genetic Diversity in Indian Land Races of Rice (Oryza sativa L). J. Plant Biochem. Biotechnol. 11, 93–97 (2002). https://doi.org/10.1007/BF03263142
Received:
Revised:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF03263142