Abstract
Thidiazuron (TDZ) stimulated shoot proliferation from different seedling explants (i.e., shoot, basal node, node and apical segment) of bamboo (Dendrocalamus strictus) when incorporated in half-strength Murashige and Skoog (MS) medium having 2% (w/v) sucrose. All the concentrations of TDZ (0.01 to 1.0 mg l−1) tried were effective in shoot proliferation. Maximum shoots (14.8 ± 1.0) were obtained from the shoot explants cultured in 0.5 mg l−1 TDZ supplemented halfstrength MS liquid medium for 21 days and subsequently transferred to the same medium devoid of TDZ. The longer culture period (i.e. 28 and 35 days) in TDZ medium caused reduction in shoot proliferation. The shoots regenerated with lower concentrations of TDZ treatment (i.e. 0.01 to 0.1 mg l−1) rooted in half-strength MS liquid medium. The shoots formed with 0.5 mg l−1 TDZ treatment did not root in basal medium and required auxin supplementation in the medium for rooting and about 55% shoots produced roots in 1.0 mg l−1 IBA supplemented medium. The shoots formed with 1.0 mg l−1 TDZ did not root even after auxin treatment. The well rooted shoots transplanted to plastic pots filled with sand and garden soil (1:1) mixture showed 98% establishment.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Abbreviations
- IAA:
-
Indole-3-acetic acid
- IBA:
-
Indole-3-butyric acid
- NAA:
-
α-Naphthalene acetic acid
- TDZ:
-
N-phenyl N’-1,2,3-thiadiazol-5-yl-urea
- MS:
-
Murashige and Skoog
References
Shirgurkar MV, Thengane SR, Poonawala IS, Jana MM, Nadgauda RS & Mascarenhas AF, Curr Sci, 70 (1996) 940.
Lin CS & Chang WC, Plant Cell Rep, 17 (1998) 617.
Rao IU, Rao IVR & Narang V, Plant Cell Rep, 4 (1985) 191.
Saxena S, Plant Cell Rep, 9 (1990) 431.
Rout GR & Des P, Plant Cell Rep, 13 (1994) 683.
Saxena S & Dhawan V, Plant Cell Rep, 18 (1999) 438.
Woods SH, Phillips GC, Woods JE & Collins GB, Plant Cell Rep, 11 (1992) 257.
Arya S, Sharma S, Kaur R & Arya ID, Plant Cell Rep, 18 (1999) 879.
Chambers SM, Heuch JHR & Pirrie A, Plant Cell Tiss Org Cult, 27 (1991) 45.
Chaturvedi HC, Sharma M & Sharma AK, Plant Sci, 91 (1993) 97.
Nadgir AL, Phadke CH, Gupta PK, Parasharami VA, Nair S & Mascarenhas AF, Silvae Genet, 33 (1984) 219.
Kanyand M, Dessai AP & Prakash CS, Plant Cell Rep, 14 (1994) 1.
Thomas JC & Katterman FR, Plant Physiol, 81 (1986) 681.
Van-Nieuwkerk JP, Zimmerman RH & Fordham I, Hort Sci, 21 (1986) 516.
Magioli C, Rocha APM, de Oliveira DE & Mansur E, Plant Cell Rep, 17 (1998) 661.
Chalupa V, Biol Plant, 29 (1987) 425.
Nielsen JIM, Hansen J & Brandt K, Plant Cell Tiss Org Cult, 41 (1995) 165.
Huetteman CA & Preece JE, Plant Cell Tiss Org Cult, 33 (1993) 105.
Lin HS, DeJeu MJ & Jacobsen E, Plant Cell Rep, 16 (1997) 770.
Murashige T & Skoog F, Physiol Plant, 15 (1962) 473.
Snedecor GW & Cochran WG, Statistical methods, Iowa University Press, Ames, Iowa (1989).
Viseur J, Acta Hortic, 212 (1987) 117.
Distabanjong K & Geneve RL, Plant Cell Tiss Org Cult, 47 (1997) 247.
Kim MK, Sommer HE, Bongarten BC & Markle SA, Plant Cell Rep, 16 (1997) 536.
Ravikumar R, Ananthakrishnan G, Kathiravan K & Ganapathi A, Plant Cell Tiss Org Cult, 52 (1998) 189.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Singh, M., Jaiswal, U. & Jaiswal, V.S. Thidiazuron-induced Shoot Multiplication and Plant Regeneration in Bamboo (Dendrocalamus strictus Nees). J. Plant Biochem. Biotechnol. 10, 133–137 (2001). https://doi.org/10.1007/BF03263122
Received:
Revised:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF03263122