Abstract
In this communication we report shoot organogenesis from hypocotyl explants in cumin (Cuminum cyminum) genotype RZ-19 by the use of thidiazuron (TDZ). Various levels of TDZ were incorporated in MS basal medium to induce regeneration. Regeneration was achieved with a frequency up to 30% on 0.5 and 0.1 mg l−1 concentration of TDZ. Shoots once produced could be multiplied on 0.5 mg l−1 kinetin (KN) at the rate of approximately 8 shoots per regenerated shoot. These multiplied shoots could go through 3–4 multiplication cycles after which they root on 1.0 mg l−1 IAA.
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References
Venkatchalam P, Rao K, Kavikishore PB & Jayabalan N, Curr Sci, 74 (1998) 61.
Kameda H & Harade H, Pflanzenphysiol, 19 (1979) 255.
Hunault G, CR Acad Sci Paris, 293 (1981) 553.
Daniel B, Simpson B, Lawarence B & Nair GM, In Biotechnology of spices, medicinal and aromatic plants (Edison et al, Editors), Indian Society of Spices, Kerala, India (1997) p 51.
Jakhar ML, Ph. D. Thesis, Rajasthan Agricultural University, Bikaner, (1999).
Wang SY, Steffens GL & Faust M, Phytochemistry, 25 (1986) 311.
Role JA, Hassan MA, Swartz HJ, Bors RH & McNicols R, Plant Cell Tiss Org Cult, 20 (1990) 223.
Saxena P K, Malik CA & Gill R, Planta, 187 (1992) 421.
Hare PD & Van Staden J, Plant Cell Physiol, 35 (1994) 1121.
Huetteman CA & Preece JE, Plant Cell Tiss Org Cult, 33 (1993) 105.
Lu CY, In Vitro Dev Biol, 29 (1993) 92.
Magioli C, Roche APM, de Oliveira, DE & mansur E, Plant Cell Rep, 17 (1998) 661.
Murashige T & Skoog F, Physiol Plant, 15 (1962) 473.
Kim MK, Sommer HE, Bongarten BC & Markle SA, Plant Cell Rep, 16 (1997) 536.
Kaneda Y, Tabei Y, Nishimura S, Harada K, Akihama T & Kitamura K, Plant Cell Rep, 17 (1997) 8.
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Gupta, D., Bhargava, S. Thidiazuron Induced Regeneration in Cuminum cyminum L. J. Plant Biochem. Biotechnol. 10, 61–62 (2001). https://doi.org/10.1007/BF03263109
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DOI: https://doi.org/10.1007/BF03263109