Abstract
TheAegilops genus contains species closely related to wheat. In common with wheat,Aegilops species accumulate high molecular weight (HMW) glutenin subunits in their endospermic tissue. In this study, we investigated the composition of HMW glutenin subunits in four multiploidAegilops species using SDS-PAGE analysis. Furthermore, by working withAe. ventricosa, we established an efficient genomic PCR condition for simultaneous amplification of DNA sequences coding for either x-or y-type HMW glutenin subunits from polyploidAegilops species. Using the genomic PCR condition, we amplified and subsequently cloned two DNA fragments that may code for HMW glutenin subunits inAe. ventricosa. Based on an analysis of the deduced amino acid sequences, we concluded that the two cloned sequences encode one x- and one y-type of HMW glutenin subunit, respectively.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Lawrence, G. J., Shepherd, K. W., Chromosomal location of genes controlling seed proteins in species related to wheat, Theor. Appl. Genet, 1981, 59: 25.
Shewry, P. R., Miflin, B. J., Seed storage proteins of economically important cereals, Adv. Cereal. Sci. Technol., 1985, 7: 1.
Weegels, P. L., Hamer, R. J., Schofield, J. D., Functional properties of wheat glutenin, J. Cereal. Sci., 1996, 23: 1.
Payne, P. I., Corfield, K. G., Holt, L. M., et al., Correlation between the inheritance of certain high-molecular-weight-subunits of glutenin and bread-making quality in progenies of six crosses of breat-wheat, J. Sci. Food Agric, 1981, 32: 51.
Blechl, A. E., Anderson, O. D., Expression of a novel high-molecular-weight glutenin subunit gene in transgenic wheat, Nature Biotechnology, 1996, 14: 875.
Altpeter, E., Vasil, V., Srivastava, V. et al., Integration and expression of the high-molecular weight-glutenin subunit lAxl gene into wheat, Nature Biotechnology, 1996: 1155.
Barro, F., Rooke, L., Békés, F. et al., Transformation of wheat with high molecular weight subunit genes results in improved functional properties, Nature Biotechnology, 1997, 15: 1295.
Miller, T. E., Systematics and evolution, in Wheat Breeding: Its Scientific Basis (ed. Lupton, F. G H.), New York: Chapman and Hall, 1987, 1–49.
William, M. D. H. M., Pena, R. J., Mujeeb-Kazi, A., Seed protein and isozyme variations inTriticum tauschii (Aegilops tauschii), Theor. Appl. Genet., 1993, 87: 257.
Mackie, A. M., Lagudah, E. S., Sharp, P. J. et al., Molecular and biochemical characterization of HMW glutenin subunits fromT. Tauschii and the D genome of hexaploid wheat, J. Cereal. Sci., 1996, 23: 213.
Mackie, A. M., Sharp, P. J., Lagudah, E. S., The nucleotide and derived amino acid sequence of an HMW glutenin gene fromTriticum tauschii and comparison with those from the D genome of bread wheat, J. Cereal. Sci., 1996, 24: 73.
Shewry, P. R., Tatham, A. S., Barro, F. et al., Biotechnology of breadmaking: unraveling and manipulating the multi-protein gluten complex, Bio/Technology, 1995, 13: 1185.
D’Ovidio, R., Anderson, O. D., PCR analysis to distinguish between alleles of a member of a multigene family correlated with wheat bread-making quality, Theor. Appl. Genet., 1994, 88: 759.
Smith, R. L., Schweder, M. E., Barnett, R. D., Identification of glutenin alleles in wheat and triticale using PCR-generated DNA markers, Crop Sci., 1994, 34: 1373.
D’Ovidio, R., Masci, S., Porceddu, E., Development of a set of oligonucleotide primers specific for genes at the Glu-1 complex loci of wheat, Theor. Appl. Genet, 1995, 91: 189.
Lafiandra, D., Tucci, G. F., Pavoni, A. et al., PCR analysis of xand ytype genes present at the complex Glu-Al locus in durum and bread wheat, Theor. Appl. Genet, 1997, 94: 235.
Payne, P. I., Holt, I. M., Law, C. N., Structural and genetic analysis on the high-molecular-weight subunits of wheat glutenin, Part 1: Allelic variation in subunits amongst varieties of wheat (Triticum aestivum), Theor. Appl. Genet, 1981, 60: 229.
McCouch, S. R., Kochert, G., Yu, Z. H. et al., Molecular mapping of rice chromosomes, Theor. Appl. Genet., 1988, 76: 815.
Sambrook, J., Fritsch, E. F., Maniatis, T., Molecular Cloning: A Laboratory Manual, New York: Cold Spring Harbor Laboratory Press, 1989.
Lutz, J., Hsam, S. L. K., Limpert, E. et al., Chromosomal location of powdery mildew resistance genes inTriticum aestivum L. (common wheat). 2. Genes Pm2 and Pml9 fromAegilops squarrosa L., Heredity, 1995, 74: 152.
Donini, P., Koebner, R. M. D., Ceoloni, C., Cytogenetic and molecular mapping of the weight-Aegilopslongissima chromatin breaking points in powdery mildew resistant introgression lines, Theor. Appl. Genet, 1995, 91: 738.
Jia, J., Devos, K. M., Chao, S. et al., RFLP-based maps of the homoeologous group-6 chromosomes of wheat and their application in the tagging of Pm12, a powdery mildew resistance gene transferred fromAegilops speltoides to wheat, Theor. Appl. Genet, 1996, 92: 559.
Ma, Z. Q., Zhao, Y. H., Sorrells, M. E., Inheritance and chromosomal location of male-fertility restoring gene transferred fromAegilops umbellulata Zhuk toTriticum aestivum L., Mol. Gen. Genet, 1995, 247: 351.
Murphy, J. P., Griffey, C. A., Finney, P. L. et al., Agronomic and grain quality evaluations ofTriticum aestivum ×Aegilops tauschii backcross populations, Crop Sci., 1997, 37: 1960.
Author information
Authors and Affiliations
Corresponding author
About this article
Cite this article
Xie, R., Wan, Y., Zhang, Y. et al. HMW glutenin subunits in multiploidAegilops species: composition analysis and molecular cloning of coding sequences. Chin.Sci.Bull. 46, 309–313 (2001). https://doi.org/10.1007/BF03187192
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF03187192