Abstract
Trichogin GA IV is one of the shortest acyclic linear polypeptide antibiotics of fungal origin, characterized by the presence of three α-amino isobutyric acid residues, an N-octanoyl group and an amino alcohol at the C terminus. Its antibiotic activity is generally thought to be based on its self-assembling and membrane-modifying properties. The technique of double electron-electron resonance in electron spin echo is used to study the spatial distribution of spin-labeled [TOAC-4]-trichogin GA IV analog bound to the cell membrane of the Gram-positive bacteriumMicrococcus luteus. The intermolecular dipole-dipole spin-spin interaction of TOAC spin labels has been experimentally studied at 77 K in glassy dispersions of the spherical cell particles. It is shown that the nonaggregated peptide molecules are distributed at the cytoplasmic membrane. Two possible distribution models are proposed: (i) the peptide molecules are randomly distributed on both inner and outer membrane surfaces with a distance between the surfaces of 7 nm, (ii) the molecules are randomly distributed in a layer up to 2.4 nm from the external surface of the membrane.
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Toniolo C., Crisma M., Formaggio F., Peggion C., Epand R.F., Epand R.M.: Cell. Mol. Life Sci.58, 1179–1188 (2001)
Epand R.F., Epand R.M., Formaggio F., Crisma M., Wu H., Lehrer R.I., Toniolo C.: Eur. J. Biochem.268, 703–712 (2001)
Auvin-Guette C., Rebuffat S., Prigent Y., Bodo B.: J. Chem. Soc. Perkin Trans. I1974, 249–255.
Monaco V., Formaggio F., Crisma M., Toniolo C., Hanson P., Millhauser G.: Biopolymers50, 239–253 (1999)
Monaco V., Formaggio F., Crisma M., Toniolo C., Hanson P., Millhauser G., George C., Deschamps J.R., Flippen-Anderson J.L.: Bioorg. Med. Chem.7, 119–131 (1999)
Milov A.D., Maryasov A.G., Tsvetkov Yu.D., Raap J.: Chem. Phys Lett.303, 135–143 (1999)
Milov A.D., Maryasov A.G., Samoilova R.I., Tsvetkov Yu.D., Raap J., Monaco V., Formaggio F., Crisma M., Toniolo C.: Dokl. Akad. Nauk.370, 265–268 (2000)
Milov A.D., Tsvetkov Yu.D., Formaggio F., Crisma M., Toniolo C., Raap J.: J. Am. Chem. Soc.122, 3843–3848 (2000)
Milov A.D., Tsvetkov Yu.D., Raap J.: Appl. Magn. Reson.19, 215–227 (2000)
Milov A.D., Tsvetkov Yu.D., Formaggio F., Crisma M., Toniolo C., Raap J.: J. Am. Chem. Soc.123, 3784–3789 (2001)
Cociancich S., Grhazi A., Hetru C., Hoffmann J.A., Letellier L.: J. Biol. Chem.268, 19239–19245 (1993)
Bourdineaud J.P., Boulanger P., Lazdunski C., Letellier L.: Proc. Natl. Acad. Sci. USA87, 1037–1041 (1990)
Surovtsev J.V., Rasumov J.A., Nekrasov V.M., Shvalov A.N., Soini J.T., Maltsev V.P., Petrov A.K., Loktev V.B., Chernyshov A.V.: J. Theor. Biol.200/3, 407–417 (2000)
Anderson D., Hanson P., McNulty J., Millhauser G., Monaco V., Formaggio F., Crisma M., Toniolo C.: J. Am. Chem. Soc.121, 6919–6927 (1999)
Milov A.D., Maryasov A.G., Tsvetkov Yu.D.: Appl. Magn. Reson.15, 107–143 (1998)
Milov A.D., Salikhov K.M., Schirov M.D.: Fiz. Tverd. Tela (Leningrad)23, 97 (1981)
Klauder J.R., Anderson P.V.: Phys. Rev.125, 912–930 (1962)
Raitsimring A.M., Salikhov K.M.: Bull. Magn. Reson.7, 912–930 (1985)
Kropacheva T.N., Raap J.: Biochim. Biophys. Acta (2002) in press.
Salton M.R.J.: J. Gen. Phys.52, 237s (1985)
Salton M.R.J.: The Bacterial Cell Wall. Amsterdam: Elsevier 1964.
Drews G. in: Procaryote Structure and Function: a New Perspective, Society for General Microbiology, Symposium 47 (Mohan S., Dow C., Cole J.A., ed.), pp. 249–274. New York: Cambridge University Press 1991.
Whiteside T.L., Siervo A.J., Salton M.R.J.: J. Bacteriol.105, 957–967 (1971)
Thorne K.J.I., Kodicek E.: Biochim. Biophys. A59, 306–312 (1962)
Caffrey M., Feigenson G.W.: Biochemistry20, 1949–1961 (1981)
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Milov, A.D., Samoilova, R.I., Tsvetkov, Y.D. et al. Spatial distribution of spin-labeled trichogin GA IV in the gram-positive bacterial cell membrane determined from PELDOR data. Appl. Magn. Reson. 23, 81–95 (2002). https://doi.org/10.1007/BF03166186
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DOI: https://doi.org/10.1007/BF03166186