Abstract
Electron paramagnetic resonance (EPR) spectroscopy at W-band (94 GHz) is used to resolve theg-tensors of the radical ions of the primary chlorophyll donor P +⋅700 and the quinone acceptor A −⋅1 in photosystem I. The obtainedg-tensor principal values are compared with those of the isolated pigment radicals in organic solvents and the shifts are related to an impact of the protein environment. P +⋅700 has been investigated in photosystem I single crystals at 94 GHz. W-band EPR applied to the photoinduced radical pair P +⋅700 A −⋅1 is used to correctly assign theg-tensor axes of P +⋅700 to the molecular structure of the primary donor. Density functional theory calculations on a model of A −⋅1 in its binding pocket derived from the recent crystal structure of photosystem I were utilized to correlate experimental magnetic resonance parameters with structural elements of the protein.
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Teutloff, C., Hofbauer, W., Zech, S.G. et al. High-frequency EPR studies on cofactor radicals in photosystem I. Appl. Magn. Reson. 21, 363–379 (2001). https://doi.org/10.1007/BF03162414
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DOI: https://doi.org/10.1007/BF03162414