Abstract
Soybean lipoxygenase-1 high frequency (≥70 GHz) EPR spectra at low magnetic field strengths (<2 Tesla) are predicted to have interdoublet transitions. Analysis of these transitions, if they are observed, will accurately measure all the zero field splitting (ZFS) terms for the second order spin Hamiltonian. The combination of interdoublet transitions with Kramers doublet transitions can be used as a sensitive,direct, measurement ofD. The non-heme iron protein, lipoxygenase, in its activatedS=5/2 Fe+3 state, exhibits distributions inE/D. The effect of distributions in ZFS parameters on Kramers doublet transitions and interdoublet transitions are very different. These differences can be used to separate the components of the distribution inE/D: distributions inE andD. A first estimate of the separated distributions can be obtained from the relative amplitudes of the lowest Kramers doublet to the interdoublet transitions.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Gerfen G.J., Bellow B.F., Un S., Bollinger J.M., Stubbe J., Griffin R.G., Singel D.J.: J. Am. Chem. Soc.115, 6420–6421 (1993)
Lynch W.B., Boorse R.S., Freed J.H.: J. Am. Chem. Soc.115, 10909–10915 (1993)
Muller F., Hopkins M.A., Coron N., Grynberg M., Brunel L.C., Martineq. G.: Rev. Sci. Instrum.60, 3681–3684 (1989)
Slappendel S., Veldink G.A., Vliegenthart J.F.G., Aasa R., Malmstrom B.G.: Biochim. Biophys. Acta642, 30–39 (1980)
Gaffney B.J., Mavrophilipos D.V., Doctor K.S.: Biophys. J.64, 773–783 (1993)
Alpert Y., Couder Y., Tuchendler J., Thome H.: Biochim. Biophys. Acta322, 34–37 (1973)
Yang A.-S., Gaffney B.J.: Biophys. J.51, 55–67 (1987)
Doctor K.S., Gaffney B.J., Alvarez G., Silverstone H.J.: J. Phys. Chem.97, 3028–3033 (1993)
Aasa R., Vänng»rd T.: J. Magn. Reson.19, 308–315 (1975)
Coffman R.E.: J. Phys. Chem.79, 1129–1136 (1975)
Fiamango F.G., Brill A.S., Hampton D.A., Thorkildsen R.: Biophys. J.55, 67–77 (1989)
Gaffney B.J., Silverstone H.J. in: Biological Magnetic Resonance, vol. 13: EMR of Paramagnetic Molecules (Berliner L.J., Reuben J., eds.), pp. 1–57. New York: Plenum Press 1993.
Pilbrow J.R., Sinclair G.R., Hutton D.R., Troup G.J.: J. Magn. Reson.52, 386–399 (1983)
Boyington J.C., Gaffney B.J., Amzel L.M.: Science260, 1482–1486 (1993)
Minor W., Steczko J., Bolin J.T., Otwinowski Z., Axelrod B.: Biochemistry29, 8885 (1993)
Pistorius E.K., Axelrod B.: J. Biol. Chem.249, 3183–3186 (1974)
Whittaker J.W., Solomon E.I.: J. Am. Chem. Soc.110, 5329–5339 (1988)
Slappendel S., Aasa R., Malmstrom B.G., Verhagen J., Veldink G.A., Vliegenthart J.F.G.: Biochim. Biophys. Acta708, 259–265 (1982)
Zhang Y., Gebbhard M.S., Solomon E.I.: J. Am. Chem. Soc.113, 5162–5175 (1991)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Doctor, K.S., Gaffney, B.J. High frequency EPR predictions for the non-heme iron protein lipoxygenase. Appl. Magn. Reson. 11, 425–435 (1996). https://doi.org/10.1007/BF03162238
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF03162238