Abstract
Ischemic rat brains were prepared by decapitation followed by incubation in an artificial cerebrospinal fluid at various times at 37°C, and the levels of phospholipids, free fatty acids, and enzymes involved in their metabolism were studied.
Activities of phospholipase A, phospholipase C, and di- and monoglyceride lipase, assayed with optimal concentrations of Ca2+ and lysophospholipase, did not significantly change by 60 min of ischemia, whereas acylation enzymes of lysophospholipid decreased in activity to an extent of 70% of control at 15 min after the ischemic treatment. The maximal activities were found at 8×10−3 M, 1×10−3 M, and 2×10−2 M Ca2+ for phospholipase A, phospholipase C, and di- and monoglyceride lipases, respectively in microsomal fractions of both control and ischemic brain.
Furthermore, the sensitivity of microsomal enzymes to endogenous Ca2+ was estimated in control and ischemic brain. The sensitivity of phospholipase C was found to be increased after 1 min of ischemic treatment, but those of phospholipase A and di- and monoglyceride lipase were not increased.
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Abbreviations
- GroPCho:
-
sn-glycero-3-phosphocholine
- GroPIns:
-
sn-glycero-3-phosphoinositol
- ChoGpl:
-
choline glycerophos-pholipids
- EtnGpl:
-
ethanolamine glycerophospholipids
- SerGpl:
-
serine glycerophospholipids
- InsGpl:
-
inositol glycerophospholipids
- PtdCho:
-
1,2-diacyl-GroPCho
- PtdEtn:
-
1,2-diacyl-GroPEtn
- PtdIns:
-
1,2-diacyl-GroPIns
- PlsEtn:
-
1-alk-1′-enyl-2-acyl-GroPEtn
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Hirashima, Y., Moto, A., Endo, S. et al. Activities of enzymes metabolizing phospholipids in rat cerebral ischemia. Molecular and Chemical Neuropathology 10, 87–100 (1989). https://doi.org/10.1007/BF03159716
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DOI: https://doi.org/10.1007/BF03159716