Abstract
The aim of this study was to investigate the expression of Ki-67, bcl-2 and p53 in parathyroid adenomas and their residual rim of normal parathyroid tissue. Specimens from 26 parathyroid adenomas were studied by immunohistochemical analysis for Ki-67, bcl-2 and p53 expression. Positive findings were noted for p53 in 4 (15%) adenomas and none of the residual rims of normal parathyroid tissue (p = 0.055); for Ki-67 in 15 (56%) adenomas and none of the residual rims of normal parathyroid tissue (p = 0.00002); and for bcl-2 in 19 (73%) adenomas and 8 (31%) residual rims of normal parathyroid tissue (p < 0.01). The high rate of Ki-67 expression may indicate susceptibility of parathyroid adenomas to clonal proliferation. The weak immunoreactive expression of p53, combined with a relatively strong expression of bcl-2, may contribute to the characteristic slow progression of these tumors.
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Abbona GC, Papotti M, Gasparri G, et al: Proliferative activity in parathyroid tumors as detected by Ki-67 immunostaining. Hum Pathol 26:135–138, 1995
Arnold A, Staunton CE, Kim HG, et al: Monoclonality and abnormal parathyroid hormone genes in parathyroid adenomas. N Engl J Med 318: 658–662, 1988
Arnold A, Kim HG: Clonal loss of one chromosome 11 in a parathyroid adenoma. J Clin Endocrinol Metab 69:496–499, 1989
Carson DA, Ribeiro JM: Apoptosis and disease. Lancet 341:1251–1254, 1993
Cryns VL, Rubio MP, Thor AD, et al: p53 abnormalities in human parathyroid carcinoma. J Clin Endocrinol Metab 78:1320–1324, 1994
Dervan PA, Magee HM, Buckley C, et al: Proliferating cell nuclear antigen counts in formalin-fixed paraffin-embedded tissue correlate with Ki-67 in fresh tissue. Am J Clin Pathol 97:S21–28, 1992
Ghandur-Mnaymneh L, Kimura N: The parathyroid adenoma. A histopathologic definition with a study of 172 cases of primary hyperparathyroidism. Am J Pathol 115:70–83, 1984
Greenblatt MS, Bennett WP, Hollstein M, et al: Mutations in the p53 tumor suppressor gene: clues to cancer etiology and molecular pathogenesis. Cancer Res 54:855–4878, 1994
Gulkesen KH, Kilicarslan B, Altunbas HA, et al: EGFR and p53 expression and proliferative activity in parathyroid adenomas; an immunohistochemical study. APMIS 109:870–874, 2001
Hakim JP, Levine MA: Absence of p53 point mutations in parathyroid adenoma and carcinoma. J Clin Endocrinol Metab 78:103–106, 1994
Hockenbery DM, Zutter M, Hickey W, et al: BCL2 protein is topographically restricted in tissues characterized by apoptotic cell death. Proc Natl Acad Sci USA 88:6961–6965, 1991
Hollstein MC, Peri L, Mandard AM, et al: Genetic analysis of human esophageal tumors from two high incidence geographic areas: frequent p53 base substitutions and absence of ras mutations. Cancer Res 51:4102–4106, 1991
Karak AK, Sarkar C, Chumber S, Tandon N: MIB-1 proliferative index in parathyroid adenoma & hyperplasia. Ind J Med Res 105: 235–238, 1997
Kayath MJ, Martin LC, Vieira JG, et al: A comparative study of p53 immunoexpression in parathyroid hyperplasias secondary to uremia, primary hyperplasias, adenomas and carcinomas. Eur J Endocrinol 139:78–83, 1998
Kishikawa S, Shan L, Ogihara K, et al: Overexpression and genetic abnormality of p53 in parathyroid adenomas. Pathol Int 49:853–857, 1999
LeBrun DP, Warnke RA, Cleary ML: Expression of bcl-2 in fetal tissues suggests a role in morphogenesis. Am J Pathol 142:743–753, 1993
Lloyd RV, Carney A, Ferreio JA, et al: Immunohistochemical Analysis of the cell cycle-associated antigens ki-67 and retinoblastoma protein in parathyroid carcinomas and adenomas. Endocrine Pathol 6: 279–287, 1995
Loda M, Lipman J, Cukor B, et al: Nodular foci in parathyroid adenomas and hyperplasias: an immunohistochemical analysis of proliferative activity. Hum Pathol 25:1050–1056, 1994
Lu QL, Abel P, Foster CS, et al: bcl-2: role in epithelial differentiation and oncogenesis. Hum Pathol 27:102–110, 1996
Martin LN, Kayath MJ, Vieira JG, et al: Parathyroid glands in uraemic patients with refractory hyperparathyroidism: histopathology and p53 protein expression analysis. Histopathology 33:46–51, 1998
Naccarato AG, Marcocci C, Miccoli P, et al: Bcl-2, p53 and MIB-1 expression in normal and neoplastic parathyroid tissues. J Endocrinol Invest 21:136–141, 1998
Nigro JM, Baker SJ, Preisinger AC, et al: Mutations in the p53 gene occur in diverse human tumor types. Nature 342:705–708, 1989
Novack DV, Korsmeyer SJ: Bcl-2 protein expression during murine development. Am J Pathol 145:61–73, 1994
Ricci F, Mingazzini PL, Sebastiani V, et al: p53 as a marker of differentiation between hyperplastic and adenomatous parathyroids. Ann Diagn Pathol 6:229–235, 2002
Stojadinovic A, Hoos A, Nissan A, et al: Parathyroid neoplasms: clinical, histopathological, and tissue microarray-based molecular analysis. Hum Pathol 34:54–64, 2003
Szende B, Farid P, Vegso G, et al: Apoptosis and P53, Bcl-2 and Bax gene expression in parathyroid glands of patients with hyperparathyroidism. Pathol Oncol Res 10: 98–103, 2004
Szende B: The occurrence and significance of apoptosis in tumors. Magy Onkol 48: 215–219, 2004
Tsujimoto Y, Cossman J, Jaffe E, et al: Involvement of the bcl-2 gene in human follicular lymphoma. Science 228:1440–1443, 1985
Vargas MP, Vargas HI, Kleiner DE, et al: The role of prognostic markers (MIB-1, RB, and bcl-2) in the diagnosis of parathyroid tumors. Mod Pathol 10:12–17, 1997
Wang W, Johansson H, Kvasnicka T, et al: Detection of apoptotic cells and expression of Ki-67 antigen, Bcl-2, p53 oncoproteins in human parathyroid adenoma. APMIS 104:789–796, 1996
Williams GT: Programmed cell death: apoptosis and oncogenesis. Cell 65:1097–1098, 1991
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Hadar, T., Shvero, J., Yaniv, E. et al. Expression of p53, Ki-67 and Bcl-2 in parathyroid adenoma and residual normal tissue. Pathol. Oncol. Res. 11, 45–49 (2005). https://doi.org/10.1007/BF03032405
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DOI: https://doi.org/10.1007/BF03032405