Abstract
We have determined that a nodule-specific cDNA clone (GmCysP1), obtained from a soybean root nodule-specific EST pool, encodes cysteine proteinase. Its amino acid sequence homology, as well as the conservation of typical motifs and amino acid residues involved in active site formation, shows that GmCysP1 can be classified as a legumain (C13) family cysteine proteinase, belonging to clan CD. Moreover, based on its expression patterns,GmCysP1 is a nodule-specific cysteine proteinase gene that is possibly associated with nodule development or senescence. Our genomic Southern analysis also suggests thatGmCysP1 is a member of a multigene family. Therefore, we propose that GmCysP1 is the first to be identified as a nodule-specific and senescence-related cysteine proteinase that belongs to the legumain family from soybean.
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Literature Cited
Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403–410
Barrett AJ, Rawlings ND (2001) Evolutionary lines of cysteine peptidases. Biol Chem 382: 727–733
Becker C, Fisher J, Nong VH, Munitz K (1994) PCR cloning and expression analysis of cDNAs encoding cysteine proteinases from germinating seeds ofVicia sativa L. Plant Mol Biol 26: 1207–1212
Chen JM, Rawlings ND, Stevens RA, Barrett AJ (1998) Identification of the active site of legumain links it to caspases, clostripain and gingipains in a new clan of cysteine endopeptidases. FEBS Lett 441: 361–365
Doyle JJ, Doyle Jl (1990) Isolation of plant DNA from fresh tissue. Focus 12: 13–15
Ehrhardt DW, Atkinson EM, Long SR (1992) Depolarization of alfalfa root hair membrane potential byRhizo-bium meliloti Nod factors. Science 256: 998–1000
Guerrero FD, Jones JT, Mullet JE (1990) Turgor-responsive gene transcription and RNA levels increase rapidly when pea shoots are wilted. Sequence and expression of three inducible genes. Plant Mol Biol 15: 11–26
Hensel LL, Grbic V, Baumgarten DA, Bleecker AB (1993) Developmental and age-related processes that influence the longevity and senescence of photosynthetic tissues in Arabidopsis. Plant Cell 5: 553–564
Higgins DG, Bleasby AJ, Fuchs R (1992) Clustal V: Improved software for multiple sequence alignment. Comput Appl Biosci 8: 189–191
Ho SL, Tong WF, Yu SM (2000) Multiple mode regulation of a cysteine proteinase gene expression in rice. Plant Physiol 122: 57–66
Kardailsky IV, Brewin NJ (1996) Expression of cysteine protease genes in pea nodule development and senescence. Mol Plant Microbe Interact 9: 689–695
Koizumi M, Yamaguchi-Shinozaki K, Tsuji H, Shinozaki K (1993) Structure and expression of two genes that encode distinct drought-inducible cysteine proteinases inArabidopsis thaliana. Gene 129: 175–82
Lee H, Hur CG, Oh CJ, Kim HB, Park S, An CS (2004a) Analysis of the root nodule-enhanced transcriptome from soybean. Mol Cells 18: 53–62
Lee H, Kim H, An CS (2004b) Cloning and expression analysis of 2-on-2 hemoglobin from soybean. J Plant Biol 47: 92–98
Linthorst HJ, van der Does C, Brederode FT, Bol JF (1993) Orcadian expression and induction by wounding of tobacco genes for cysteine proteinase. Plant Mol Biol 21: 685–694
Mellor RB (1989) Bacteroids in the Rhizobium-legume symbiosis inhabit a plant internal lytic compartment: Implications for other microbial endosymbioses. J Exp Bot 40: 831–839
Naito Y, Fujie M, Usami S, Murooka Y, Yamada T (2000) The involvement of a cysteine proteinase in the nodule development in Chinese milk vetch infected withMesorhizobium huakuii subsp. rengei. Plant Physiol 124: 1087–1096
Nong VH, Becker C, Muntz K (1995) cDNA cloning for a putative cysteine proteinase from developing seeds of soybean. Biochim Biophys Acta 1261: 435–438
Pechan T, Jiang B, Steckler D, Ye L, Lin L, Luthe DS, Williams WP (1999) Characterization of three distinct cDNA clones encoding cysteine proteinases from maize (Zea mays L.) callus. Plant Mol Biol 40: 111–119
Rawlings ND, Tolle DP, Barrett A) (2004) MEROPS: The peptidase database. Nucleic Acids Res 32 Database issue: D160–164
Roth LE, Stacey G (1989) Bacterium release into host cells of nitrogen-fixing soybean nodules: The symbiosome membrane comes from three sources. Eur J Cell Biol 46: 12–23
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, New York
Schaffer MA, Fischer RL (1988) Analysis of mRNA that accumulate in response to low temperature identifies a thiol protease gene in tomato. Plant Physiol 87: 431–436
Seo S, Tan-Wilson A, Wilson KA (2001) Protease C2, a cysteine endopeptidase involved in the continuing mobilization of soybean beta-conglycinin seed proteins. Biochim Biophys Acta 1545: 192–206
Shimada T, Hiraiwa N, Nishimura M, Hara-Nishimura I (1994) Vacuolar processing enzyme of soybean that converts proproteins to the corresponding mature forms. Plant Cell Physiol 35: 713–718
Uhde-Stone C, Zinn KE, Ramirez-Yanez M, Li A, Vance CR, Allan DL (2003) Nylon filter arrays reveal differential gene expression in proteoid roots of white lupin in response to phosphorus deficiency. Plant Physiol 131: 1064–1079
Vincent JL, Brewin NJ (2000) Immunolocalization of a cysteine protease in vacuoles, vesicles, and symbiosomes of pea nodule cells. Plant Physiol 123: 521–530
Vincent JM (1970) A Manual for the Practical Study of Root-Nodule Bacteria. I.B.P. Handbook No 15. Black-well, Oxford
Wittenbach VA, Lin W, Hebert R (1982) Vacuolar localization of proteases and degradation of chloroplasts in mesophyll protoplasts from senescing primary wheat leaves. Plant Physiol 69: 98–102
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Oh, C.J., Lee, H., Kim, H.B. et al. Isolation and characterization of a root nodule-specific cysteine proteinase cDNA from soybean. J. Plant Biol. 47, 216–220 (2004). https://doi.org/10.1007/BF03030511
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DOI: https://doi.org/10.1007/BF03030511