Abstract
Purpose
To investigate the incidence of tumour metastasis from B16 melanoma tumour cells in expenmental animals following exposure to equipotent concentrations of halothane or isoflurane, and to differentiate if exposure to one anaesthetic resulted in greater metastases than the other.
Methods
Experimental animals (C57B1 mice), were randomized to receive 1.3 MAC hours of halothane or isoflurane anaesthesia. The control group of animals received oxygen alone under identical conditions. Fifteen minutes after completion of anaesthesia, control and experimental groups were given 1 × 105 B16 melanoma cells intravenously. After 21 days, all animals were autopsied, and the metastatic nodules in their lungs were counted. The difference in the numbers of metastatic nodules between control and expenmental groups of animals was analyzed for significance by the Mann Whitney “U test”.
Results
More metastases were observed in the animals exposed to halothane (37.28±5.08, P< 0.0001), or isoflurane anaesthesia (28.24±4.07, P< 0.0014) than in the control animals (12.22± 1.52).
Conclusion
Exposure to halothane or isoflurane anaesthesia increased the number of pulmonary metastases in C57B1 mice compared with the control groups but there was no difference in metastases among animals treated with halothane or isoflurane.
Résumé
Objectif
Rechercher l’incidence des métastases de cellules tumorales mélanomateuses B16 sur des animaux de laboratoire après l’exposition à des concentrations équipotentes d’halothane et d’isoflurane, et vérifier si l’exposition à un anesthésique produisait un plus grand nombre de métastases qu’à l’autre.
Méthodes
Des animaux de laboratoires (souris 57BI) étaient réparties aléatoirement pour recevoir 1, 3 MAC heures d’anesthésie à l’halothane ou à l’isoflurane. Le groupe contrôle ne recevait que de l’oxygène dans des conditions expérimentales identiques. Quinze minutes après l’arrêt de l’anesthésie, les souris des groupes contrôle et expérimentaux recevaient 1 × 105 de cellules mélanomateuses B16 par la voie intraveineuse. Après 21 jours, tous les animaux étaient autopsiés et les nodules pulmonaires métastatiques dénombrés. La signification statistique de la différence entre le nombre nodules métastatiques dénombrés entre le groupe contrôle et les groupes expénmentaux était déterminée avec le test U de Mann Whitney.
Résultats
On a observé plus de métastases chez les animaux exposés à l’halothane (37, 28±5, 08, P< 0, 0001) ou à l’isoflurane (28, 24±4, 07, P< 0.0014) que dans le groupe contrôle (12, 22± 1, 52).
Conclusion
L’exposition à l’halothane ou à l’isoflurane augmente le nombre de métastases pulmonaires chez de souris C57BI comparativement au groupe contrôle mais il n’y a aucune différence entre les animaux anesthésiés que soit à l’halothane ou à l’isoflurane.
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Moudgil, G.C., Singal, D.P. Halothane and isoflurane enhance melanoma tumour metastasis in mice. Can J Anesth 44, 90–94 (1997). https://doi.org/10.1007/BF03014331
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DOI: https://doi.org/10.1007/BF03014331